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Hamster Interleukin-4(IL4) ELISA kit

Protocol MSDS
Code CSB-EL011659HA
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 4
Alternative Names
IL4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit
Abbreviation
IL4
Uniprot No.
Q60440
Species
Mesocricetus auratus (Golden hamster)
Sample Types
serum, plasma, tissue homogenates
Detection Range
9.37 pg/mL-600 pg/mL
Sensitivity
2.34 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 96
Range % 90-101
1:2 Average % 102
Range % 97-107
1:4 Average % 100
Range % 98-104
1:8 Average % 94
Range % 87-97
Recovery
The recovery of hamster IL4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 93-99
EDTA plasma (n=4) 91 87-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
600 1.820 1.864 1.842 1.628
300 1.357 1.289 1.323 1.109
150 0.930 0.963 0.947 0.733
75 0.706 0.739 0.723 0.509
37.5 0.503 0.524 0.514 0.300
18.75 0.392 0.384 0.388 0.174
9.37 0.304 0.288 0.296 0.082
0 0.216 0.212 0.214 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Hamster IL4 ELISA Kit was designed for the quantitative measurement of Hamster IL4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.37 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.

Related Products

IL4 Proteins

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Target Background

Function
(From Uniprot)
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
Subcellular Location
Secreted.
Protein Families
IL-4/IL-13 family
Promotions
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Related pathways
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