Horse Fibrinogen(Fbg)ELISA Kit

Code CSB-E17079Hs
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
Fibrinogen(Fbg)
Abbreviation
Fbg
Species
Equus caballus (Horse)
Sample Types
serum, plasma, urine
Detection Range
23.4 ng/mL-1500 ng/mL
Sensitivity
23.4 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse fibrinogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Sample

Serum(n=4)

1:5000

Average %

93

Range %

88-99

1:10000

Average %

97

Range %

91-105

1:20000

Average %

89

Range %

82-96

1:40000

Average %

96

Range %

90-102

Recovery

The recovery of horse fibrinogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

95

89-98

EDTA plasma (n=4)

98

93-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

1500

0.149

0.154

0.151

750

0.226

0.237

0.231

375

0.301

0.320

0.310

187.5

0.436

0.429

0.432

93.75

0.597

0.611

0.604

46.8

0.876

0.853

0.865

23.4

1.294

1.284

1.289

0

2.338

2.225

2.282

Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Horse Fbg ELISA Kit was designed for the quantitative measurement of Horse Fbg protein in serum, plasma, urine. It is a Competitive ELISA kit, its detection range is 23.4 ng/mL-1500 ng/mL and the sensitivity is 23.4 ng/mL.

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