Mouse Fibrinogen,Fbg ELISA Kit

Code CSB-E08202m
Size 96T,5×96T,10×96T
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Product Details

Target Name
Fibrinogen,Fbg
Abbreviation
Fbg
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 μg/mL-10 μg/mL
Sensitivity
0.039 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Fbg in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:200Average %95
Range %87-103
1:400Average %92
Range %84-105
1:800Average %96
Range %83-106
1:1600Average %93
Range %88-104
Recovery
The recovery of mouse Fbg spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9790-108
EDTA plasma (n=4)9688-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/mlOD1OD2AverageCorrected
102.672 2.711 2.692 2.559
51.958 2.013 1.986 1.853
2.51.275 1.282 1.279 1.146
1.250.729 0.764 0.747 0.614
0.6250.437 0.434 0.436 0.303
0.3120.365 0.349 0.357 0.224
0.1560.259 0.247 0.253 0.120
00.130 0.135 0.133
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Fibrinogen is a soluble plasma glycoprotein that plays a central role in hemostasis and blood coagulation. During the coagulation cascade, fibrinogen is converted to fibrin by thrombin, forming the structural framework of blood clots. Beyond its hemostatic function, fibrinogen serves as an acute-phase reactant. Elevated levels occur during inflammation, infection, and tissue injury. Research has shown fibrinogen's involvement in cardiovascular disease, wound healing, and various pathological conditions, making it an important biomarker for studying coagulation disorders and inflammatory responses.

The Mouse Fibrinogen ELISA Kit (CSB-E08202m) is designed for quantitative measurement of fibrinogen in mouse serum, plasma, and tissue homogenates. This sandwich-based assay offers a detection range of 0.156-10 μg/mL with a sensitivity of 0.039 μg/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength. This provides researchers with a reliable tool for fibrinogen quantification in Mus musculus samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research investigating coagulation and hemostatic processes, particularly in studies examining inflammatory responses and thrombotic mechanisms. The kit allows quantification of fibrinogen levels in mouse plasma samples collected from experimental animal models. Research applications have focused on understanding the relationship between inflammation and coagulation cascade activation.

Inflammatory coagulation research: Measuring fibrinogen levels alongside other coagulation markers in studies examining the interplay between inflammatory cytokines and hemostatic factors
Thrombotic biomarker profiling: Quantifying fibrinogen as part of comprehensive coagulation panels that include multiple hemostatic parameters in mouse plasma
Experimental animal studies: Analyzing fibrinogen concentrations in mouse models to investigate coagulation responses following surgical interventions or other experimental treatments

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