Code | CSB-EL018724HO |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<15% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 93 | |||||
Range % | 88-101 | ||||||
1:2 | Average % | 95 | |||||
Range % | 85-100 | ||||||
1:4 | Average % | 91 | |||||
Range % | 89-99 | ||||||
1:8 | Average % | 93 | |||||
Range % | 90-100 |
The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 96 | 91-105 | |||||
EDTA plasma (n=4) | 97 | 88-102 | |||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Horse PRL ELISA Kit was designed for the quantitative measurement of Horse PRL protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-1000 ng/mL and the sensitivity is 2.5 ng/mL.
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Trying to determine whether this CSB-EL018724HO Horse Prolactin(PRL) ELISA kit would work with rhinoceros samples. What antigen was used to create the capture antibody? Is the capture antibody monoclonal or polyclonal? What is the competitive antigen?
The product CSB-EL018724HO is a competitive kit. The plate is coated with goat-anti-rabbit antibody (Immunogen is rabbit IgG).
The specific antibody used in the kit is a rabbit polyclonal antibody.
Immunogen is a CHO-derived recombinant protein.
The Standard is CHO-derived recombinant protein.
For rhinoceros samples, we didn't test with bovine or horse ELISA kits. We suggest you do a pretest first if you want to have a try.