Horse Prolactin(PRL) ELISA kit

Code CSB-EL018724HO
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Prolactin(PRL)
Alternative Names
PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
Abbreviation
PRL
Uniprot No.
Species
Equus caballus (Horse)
Sample Types
serum, plasma
Detection Range
2.5 ng/mL-1000 ng/mL
Sensitivity
2.5 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 88-101
1:2 Average % 95
Range % 85-100
1:4 Average % 91
Range % 89-99
1:8 Average % 93
Range % 90-100
Recovery
The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-105
EDTA plasma (n=4) 97 88-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
0 2.133 2.121 2.127
2.5 1.516 1.501 1.509
8.75 0.996 1.002 0.999
31.25 0.538 0.555 0.547
187.5 0.341 0.339 0.340
1000 0.162 0.159 0.161
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Horse PRL ELISA Kit was designed for the quantitative measurement of Horse PRL protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-1000 ng/mL and the sensitivity is 2.5 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

Trying to determine whether this CSB-EL018724HO Horse Prolactin(PRL) ELISA kit would work with rhinoceros samples. What antigen was used to create the capture antibody? Is the capture antibody monoclonal or polyclonal? What is the competitive antigen?

A:

The product CSB-EL018724HO is a competitive kit. The plate is coated with goat-anti-rabbit antibody (Immunogen is rabbit IgG).
The specific antibody used in the kit is a rabbit polyclonal antibody.
Immunogen is a CHO-derived recombinant protein.
The Standard is CHO-derived recombinant protein.
For rhinoceros samples, we didn't test with bovine or horse ELISA kits. We suggest you do a pretest first if you want to have a try.

Target Background

Function
(From Uniprot)
Prolactin acts primarily on the mammary gland by promoting lactation.
Subcellular Location
Secreted.
Protein Families
Somatotropin/prolactin family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1