Horse Prolactin(PRL) ELISA kit

Instructions
Code CSB-EL018724HO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Prolactin(PRL)
Alternative Names PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
Abbreviation PRL
Uniprot No. P12420
Species Equus caballus (Horse)
Sample Types serum, plasma
Detection Range 2.5 ng/mL-1000 ng/mL
Sensitivity 2.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of horse PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 88-101
1:2 Average % 95
Range % 85-100
1:4 Average % 91
Range % 89-99
1:8 Average % 93
Range % 90-100
Recovery
The recovery of horse PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 91-105
EDTA plasma (n=4) 97 88-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
0 2.133 2.121 2.127
2.5 1.516 1.501 1.509
8.75 0.996 1.002 0.999
31.25 0.538 0.555 0.547
187.5 0.341 0.339 0.340
1000 0.162 0.159 0.161
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Background

Function
(From Uniprot)
Prolactin acts primarily on the mammary gland by promoting lactation.
Subcellular Location Secreted.
Protein Families Somatotropin/prolactin family
Database Links

KEGG: ecb:100034034

STRING: 9796.ENSECAP00000000120

UniGene: Eca.12783

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