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Code | CSB-E12100h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human 8-iso-PGF2a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 90-100 | |||||
1:2 | Average % | 92 | ||||
Range % | 85-99 | |||||
1:4 | Average % | 95 | ||||
Range % | 90-103 | |||||
1:8 | Average % | 94 | ||||
Range % | 90-99 |
The recovery of human 8-iso-PGF2a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 88-102 | ||||
EDTA plasma (n=4) | 97 | 91-103 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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This Human 8-iso-PGF2a ELISA Kit was designed for the quantitative measurement of Human 8-iso-PGF2a protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 125 pg/mL-5000 pg/mL and the sensitivity is 125 pg/mL.
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