Code | CSB-E11172h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 92 | ||||
Range % | 87-96 | |||||
1:2 | Average % | 106 | ||||
Range % | 98-109 | |||||
1:4 | Average % | 92 | ||||
Range % | 88-95 | |||||
1:8 | Average % | 96 | ||||
Range % | 90-101 |
The recovery of human ADAM10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 84-94 | ||||
EDTA plasma (n=4) | 94 | 90-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The ELISA Kit is designed for quantitatively measuring human ADAM10 levels in samples, including serum, plasma, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADAM10 in samples. The color intensity is measured at 450 nm via a microplate reader.
ADAM10, a member of the large family of ADAMs, is expressed at high levels in the brain and modulates the molecular organization and activity of the excitatory synapse by shedding postsynaptic proteins, including N-cadherin (N-CAD), amyloid precursor protein, nectin-1, prion protein, neuroligin-1, neural cell adhesion molecule L1, and ephrin A2 and A5. ADAM10-mediated shedding of cell-adhesion molecules at the synapse activates a number of processes critical to the proper formation, maintenance, and function of the excitatory synaptic circuitries. ADAM10 is essential for development because it cleaves Notch proteins to induce Notch signaling and regulate cell fate decisions. Impairments in ADAM10 level and/or activity are detrimental to the human brain. ADAM10 has been associated with epilepsy, Alzheimer's disease, and the developmental disorder Fragile X syndrome.
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