Human A disintegrin and metalloproteinase with thrombospondin motifs 9(ADAMTS9) ELISA kit

Code CSB-EL001316HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
ADAM metallopeptidase with thrombospondin type 1 motif, 9
Alternative Names
ADAMTS9 ELISA Kit; KIAA1312 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs 9 ELISA Kit; ADAM-TS 9 ELISA Kit; ADAM-TS9 ELISA Kit; ADAMTS-9 ELISA Kit; EC 3.4.24.- ELISA Kit
Abbreviation
ADAMTS9
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTS9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %90
Range %85-96
1:10Average %95
Range %91-100
1:20Average %92
Range %86-98
1:40Average %90
Range %86-98
Recovery
The recovery of human ADAMTS9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10097-106
EDTA plasma (n=4)8794-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.500 2.488 2.494 2.398
251.458 1.376 1.417 1.321
12.50.717 0.684 0.701 0.605
6.250.358 0.339 0.349 0.253
3.120.227 0.214 0.221 0.125
1.560.161 0.159 0.160 0.064
0.780.126 0.118 0.122 0.026
00.097 0.095 0.096  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human ADAMTS9 ELISA Kit was designed for the quantitative measurement of Human ADAMTS9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

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Target Background

Function
(From Uniprot)
Cleaves the large aggregating proteoglycans, aggrecan (at the '1838-Glu-|-Ala-1839' site) and versican (at the '1428-Glu-|-Ala-1429' site). Has a protease-independent function in promoting the transport from the endoplasmic reticulum to the Golgi apparatus of a variety of secretory cargos.
Gene References into Functions
  1. ADAMTS9 gene cytosine-adenine repeat polymorphism may be used to determine the prognosis for knee osteoarthritis radiologic progression. PMID: 27230574
  2. expression by synovial cells induced by hemoglobin at low doses, suggesting a possible role for hemoglobin in cartilage damage after intra-articular hemorrhage PMID: 29137610
  3. By inhibiting ADAMTS-9, miR-338-5p suppressed the proliferation and metastasis of rheumatoid arthritis synovial fibroblasts. PMID: 28850027
  4. Exogenous expression of ADAMTS9 in colorectal cancer cell lines inhibited cell proliferation and migration through the regulation of cell cycle and apoptosis. PMID: 29186710
  5. Our data showed that four SNPs (rs73832338, rs9985304, rs4317088, and rs9831846) in the ADAMTS9 gene were significantly associated with cognitive aging among the subjects. Additionally, we found that interactions between the ADAMTS9 rs9985304 and ADAMTS9 rs76346246 SNPs influenced cognitive aging. PMID: 28225792
  6. we identified the top 10 highly differentiated SNPs in Brazilian Amerindian ancestry compared to Asian, European, and African Genomes.SNPs within or proximal to CIITA (rs6498115), SMC6 (rs1834619), and KLHL29 (rs2288697) were most differentiated in the Amerindian-specific branch. SNPs in ADAMTS9 (rs7631391), DOCK2 (rs77594147), SLC28A1 (rs28649017), ARHGAP5 (rs7151991), and CIITA (rs45601437) in the Asian comparison. PMID: 28100790
  7. study identified a suggestive genome-wide significant association of ADAMTS9 with asthma in Spanish subjects PMID: 26620591
  8. NF-kappaBp65 bound to elements located at -1177 and -1335 in the ADAMTS9 promoter region, in contrast to the untreated samples. The results of the present study suggested that NF-kappaB may be involved in IL-1beta-induced activation of ADAMTS9 in human chondrocytes PMID: 25760020
  9. A deletion at ADAMTS9-MAGI1 locus is associated with psoriatic arthritis risk. PMID: 25990289
  10. LncRNA ADAMTS9-AS2 is regulated by DNMT1 and inhibits migration of glioma cells. PMID: 24833086
  11. The present study reveals ADAMT-9 expression by mast cells(MCs) and that MC activation regulates the expression of the protease, thus implicating the ADAMT-9 of protease in MC function. PMID: 23154421
  12. The pathways MAPK and NF-kappaB were responsible pathways for the induction of ADAMTS9 gene. PMID: 23175174
  13. PPARG2 and ADAMTS9 variants are both associated with type 2 diabetes mellitus and with insulin resistance, whereas only ADAMTS9 may be related to beta cell function. PMID: 23161442
  14. ADAMTS9 acts as a functional tumor suppressor in gastric cancer through inhibiting oncogenic AKT/mTOR signaling pathway PMID: 22907434
  15. ADAMTS9 and GON-1 in the ER that promotes protein transport from the ER to the Golgi. This function is GON-domain dependent but protease activity independent. PMID: 22419820
  16. Data show that the expression of ADAMTS4, 9, 16 and was up-regulated during chondrogenesis, ADAMTS1 and 5 were down-regulated. PMID: 22562232
  17. four of the six aggrecanases are expressed in immortalized chondrocyte cell-lines and can be upregulated in response to inflammatory cytokines PMID: 20568084
  18. Results indicate that ADAMTS9 contributes an important function in the tumor microenvironment that acts to inhibit angiogenesis and tumor growth in both esophageal cancer and nasophageal cancer. PMID: 20551050
  19. These data identify ADAMTS9 as a novel, constitutive, endogenous angiogenesis inhibitor that operates cell-autonomously in endothelial cells. PMID: 20093484
  20. These data identify for the first time the cellular chaperones associated with secretion of an ADAMTS protease and suggest a role for gp96 in modulating pro-ADAMTS9 processing. PMID: 19875450
  21. ancillary domains of ADAMTS-9 are required both for specific extracellular localization and for its versicanase and aggrecanase activities PMID: 12514189
  22. negative effect of TGFbeta1 on ADAMTS-1, -5, -9, and -15 coupled with increases in their inhibitor, TIMP-3 may aid the accumulation of versican in the stromal compartment of the prostate in BPH and prostate cancer PMID: 15599946
  23. TSR-1 domain-bearing truncated ADAMTS-9 demonstrates positive GAG-binding ability & displayed low aggrecanase activity. PMID: 16507336
  24. pro-ADAMTS9 is processed at the cell surface by furin PMID: 16537537
  25. this study identifies and provides functional evidence for a critical region associated with tumor suppression on 3p14.2 and provides the first evidence that ADAMTS9, mapping to this region, may contribute to esophageal cancer development PMID: 16799631
  26. ADAMTS9 expression was downregulated or lost in 17 of 23 (73.9%) lymph node metastatic nasopharyngeal carcinoma (NPC) specimens. After transfection of the ADAMTS9 gene into 7 NPC cell lines, a dramatic reduction of colony forming ability was observed. PMID: 18449890
  27. Study show that polymorphisms in ADAMTS9 were associated with type 2 diabetes risk in the studied population. PMID: 18694974
  28. These findings may provide a better understanding of the NFATc1 regulation of ADAMTS9 expression induced by inflammatory cytokines such as IL-1 beta. PMID: 19052845
  29. present study is novel in evaluating the prevalence of ADAMTS9 methylation based on a large number of tumor samples and showing that epigenetic regulation of ADAMTS9 was associated with carcinogenesis PMID: 19963134

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Endoplasmic reticulum.
Tissue Specificity
Highly expressed in all fetal tissues. Expressed in a number of adult tissues with highest expression in heart, placenta and skeletal muscle.
Database Links

HGNC: 13202

OMIM: 605421

KEGG: hsa:56999

STRING: 9606.ENSP00000418735

UniGene: Hs.656071

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