Human A-kinase anchor protein 4(AKAP4) ELISA kit

Instructions
Code CSB-EL001526HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name A kinase (PRKA) anchor protein 4
Alternative Names A kinase (PRKA) anchor protein 4 ELISA Kit; A kinase anchor protein 4 ELISA Kit; A kinase anchor protein 82 kDa ELISA Kit; A-kinase anchor protein 4 ELISA Kit; A-kinase anchor protein 82 kDa ELISA Kit; AKAP 4 ELISA Kit; AKAP 82 ELISA Kit; AKAP-4 ELISA Kit; AKAP4 ELISA Kit; AKAP4_HUMAN ELISA Kit; AKAP82 ELISA Kit; FSC 1 ELISA Kit; FSC1 ELISA Kit; hAKAP 82 ELISA Kit; hAKAP82 ELISA Kit; HI ELISA Kit; Major sperm fibrous sheath protein ELISA Kit; p82 ELISA Kit; PRKA 4 ELISA Kit; PRKA4 ELISA Kit; Protein kinase A anchoring protein 4 ELISA Kit; Protein kinase A-anchoring protein 4 ELISA Kit; Testis specific gene HI ELISA Kit
Abbreviation AKAP4
Uniprot No. Q5JQC9
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %90-99
1:2Average %89
Range %85-96
1:4Average %105
Range %100-114
1:8Average %85
Range %80-89
Recovery
The recovery of human AKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9893-102
EDTA plasma (n=4)9288-87
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
16002.339 2.257 2.298 2.159
8001.813 1.676 1.745 1.606
4001.156 1.124 1.140 1.001
2000.699 0.743 0.721 0.582
1000.387 0.415 0.401 0.262
500.297 0.304 0.301 0.162
250.215 0.209 0.212 0.073
00.137 0.141 0.139
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
Gene References into Functions
  1. High AKAP4 expression is associated with reduced sperm motility. PMID: 29581387
  2. A significant association was found between AKAP4 gene expression and metastasis (P-value: 0.045), expression of the CTAG1B (NY-ESO-1) gene was not observed in our cases. PMID: 29480665
  3. the physiological role of the negative crosstalk between the cAMP/PKA/AKAP4 and the PKC/ERK1/2 pathways is to regulate capacitation and acrosome reaction. PMID: 27901058
  4. AKAP4 role in the proliferation and metastasis of thyroid cancer.AKAP4 is highly expressed in thyroid cancer. PMID: 27983916
  5. AKAP4 appears to be a novel Colorectal cancer-associated antigen with a potential for developing as a new clinical therapeutic target PMID: 26590805
  6. AKAP4 is a highly accurate biomarker for the detection of early stage lung cancer. PMID: 26160834
  7. SP17/AKAP4/PTTG1 are expressed in both human NSCLC cell lines and primary tumors and can elicit an immunogenic response in lung cancer patients. PMID: 25739119
  8. The putative role of AKAP4 in early tumorigenesis is implicated as a biomarker and immunotherapeutic target for cervical cancer. PMID: 23478221
  9. Ablation of AKAP4 protein caused significant inhibition in cellular proliferation, colony-forming ability, migration and invasion ability of tumor cells. PMID: 23764900
  10. data suggests that AKAP4 may be used as serum based diagnostic test for an early detection and diagnosis of breast cancer and may be a potential target for immunotherapeutic use PMID: 23451156
  11. We demonstrate the aberrant expression of AKAP-4 in prostate cancer, which will potentially be developed as a biomarker in prostate cancer. PMID: 21520158
  12. AKAP4 is a novel target for protein S-nitrosylation in spermatozoa. PMID: 17683036
  13. Role of AKAP4 in sperm motility is unclear, vut absent or weak AKAP4-labelling is associated with absent or weak sperm. motility. PMID: 17712481

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Subcellular Location Cell projection, cilium, flagellum
Protein Families AKAP110 family
Tissue Specificity Testis specific; only expressed in round spermatids.
Database Links

HGNC: 374

OMIM: 300185

KEGG: hsa:8852

STRING: 9606.ENSP00000351327

UniGene: Hs.97633

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