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Name: Human A-kinase anchor protein 4(AKAP4) ELISA kit
Brand: CUSABIO
SKU: CSB-EL001526HU

Product Details

Target Name

A kinase (PRKA) anchor protein 4

Alternative Names

A kinase (PRKA) anchor protein 4 ELISA Kit; A kinase anchor protein 4 ELISA Kit; A kinase anchor protein 82 kDa ELISA Kit; A-kinase anchor protein 4 ELISA Kit; A-kinase anchor protein 82 kDa ELISA Kit; AKAP 4 ELISA Kit; AKAP 82 ELISA Kit; AKAP-4 ELISA Kit; AKAP4 ELISA Kit; AKAP4_HUMAN ELISA Kit; AKAP82 ELISA Kit; FSC 1 ELISA Kit; FSC1 ELISA Kit; hAKAP 82 ELISA Kit; hAKAP82 ELISA Kit; HI ELISA Kit; Major sperm fibrous sheath protein ELISA Kit; p82 ELISA Kit; PRKA 4 ELISA Kit; PRKA4 ELISA Kit; Protein kinase A anchoring protein 4 ELISA Kit; Protein kinase A-anchoring protein 4 ELISA Kit; Testis specific gene HI ELISA Kit

Abbreviation

AKAP4

Uniprot No.

Q5JQC9

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

25 pg/mL-1600 pg/mL

Sensitivity

6.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-99
1:2	Average %	89
	Range %	85-96
1:4	Average %	105
	Range %	100-114
1:8	Average %	85
	Range %	80-89

Recovery

The recovery of human AKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	92	88-87

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.339	2.257	2.298	2.159
800	1.813	1.676	1.745	1.606
400	1.156	1.124	1.140	1.001
200	0.699	0.743	0.721	0.582
100	0.387	0.415	0.401	0.262
50	0.297	0.304	0.301	0.162
25	0.215	0.209	0.212	0.073
0	0.137	0.141	0.139

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

5-7 working days

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Target Background

Function (From Uniprot)	Major structural component of sperm fibrous sheath. Plays a role in sperm motility.
Gene References into Functions	High AKAP4 expression is associated with reduced sperm motility. PMID: 29581387 A significant association was found between AKAP4 gene expression and metastasis (P-value: 0.045), expression of the CTAG1B (NY-ESO-1) gene was not observed in our cases. PMID: 29480665 the physiological role of the negative crosstalk between the cAMP/PKA/AKAP4 and the PKC/ERK1/2 pathways is to regulate capacitation and acrosome reaction. PMID: 27901058 AKAP4 role in the proliferation and metastasis of thyroid cancer.AKAP4 is highly expressed in thyroid cancer. PMID: 27983916 AKAP4 appears to be a novel Colorectal cancer-associated antigen with a potential for developing as a new clinical therapeutic target PMID: 26590805 AKAP4 is a highly accurate biomarker for the detection of early stage lung cancer. PMID: 26160834 SP17/AKAP4/PTTG1 are expressed in both human NSCLC cell lines and primary tumors and can elicit an immunogenic response in lung cancer patients. PMID: 25739119 The putative role of AKAP4 in early tumorigenesis is implicated as a biomarker and immunotherapeutic target for cervical cancer. PMID: 23478221 Ablation of AKAP4 protein caused significant inhibition in cellular proliferation, colony-forming ability, migration and invasion ability of tumor cells. PMID: 23764900 data suggests that AKAP4 may be used as serum based diagnostic test for an early detection and diagnosis of breast cancer and may be a potential target for immunotherapeutic use PMID: 23451156 We demonstrate the aberrant expression of AKAP-4 in prostate cancer, which will potentially be developed as a biomarker in prostate cancer. PMID: 21520158 AKAP4 is a novel target for protein S-nitrosylation in spermatozoa. PMID: 17683036 Role of AKAP4 in sperm motility is unclear, vut absent or weak AKAP4-labelling is associated with absent or weak sperm. motility. PMID: 17712481 Show More Hide All
Subcellular Location	Cell projection, cilium, flagellum
Protein Families	AKAP110 family
Tissue Specificity	Testis specific; only expressed in round spermatids.
Database Links	HGNC: 374 OMIM: 300185 KEGG: hsa:8852 STRING: 9606.ENSP00000351327 UniGene: Hs.97633

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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Check Code：

Code	CSB-EL001526HU
Size	96T,5×96T,10×96T
Trial Size
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