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Human ADAMTS-like protein 2(ADAMTSL2) ELISA kit

Citations (1) Protocol MSDS
Code CSB-EL001318HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
ADAMTS-like 2
Alternative Names
ADAMTS like 2 ELISA Kit; ADAMTS like protein 2 ELISA Kit; ADAMTS-like protein 2 ELISA Kit; ADAMTSL 2 ELISA Kit; ADAMTSL-2 ELISA Kit; ADAMTSL2 ELISA Kit; ATL2_HUMAN ELISA Kit; FLJ45164 ELISA Kit; KIAA0605 ELISA Kit
Abbreviation
ADAMTSL2
Uniprot No.
Q86TH1
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTSL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:1 Average % 99
Range % 94-106
1:2 Average % 88
Range % 84-93
1:4 Average % 92
Range % 87-98
1:8 Average % 102
Range % 98-106
Recovery
The recovery of human ADAMTSL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-96
EDTA plasma (n=4) 86 81-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 1.854 1.988 1.921 1.812
100 1.262 1.192 1.227 1.118
50 0.780 0.733 0.757 0.648
25 0.487 0.451 0.469 0.360
12.5 0.312 0.334 0.323 0.214
6.25 0.231 0.217 0.224 0.115
3.12 0.169 0.160 0.165 0.056
0 0.109 0.108 0.109 　
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Human ADAMTSL2 ELISA Kit was designed for the quantitative measurement of Human ADAMTSL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

Citations

Related Products

ADAMTSL2 Antibodies

ADAMTSL2 Proteins

Customer Reviews and Q&A

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■ Q&A
Q:

We are looking into kits to measure ADAMTSL2. We found this product: CSB-EL001318HU - has this kit measured endogenous ADAMTSL2 in plasma or serum and what is the immunogen/calibrator used in this kit? I assume full-length recombinant in some expression system?

A:

The standard of this kit is NS0-derived recombinant protein.
And the kit can detect the ADAMTSL2 protein content in natural samples (serum plasma, etc.), but the status of each sample is different, please check your own actual measurement.
Immunogen: NS0-derived recombinant protein

Target Background

Gene References into Functions
  1. CpG-specific DNA methylation of ADAMTSL2 and BTN3A2 at rheumatoid arthritis diagnosis can serve as a marker of treatment response. PMID: 28447857
  2. Two compound heterozygous mutations were confirmed in the ADAMTSL2 gene of the patient with geleophysic dysplasia. PMID: 28917829
  3. A novel mutation in ADAMTSL2 (p. Gly421Ser) gene was identified in individuals with Ehlers-Danlos Syndrome. PMID: 26879370
  4. Two novel homozygous missense mutations in the ADAMTSL2 gene underlie geleophysic dysplasia in two consanguineous families from the United Arab Emirates. PMID: 24014090
  5. Molecular screening of ADAMTSL2 gene in 33 patients reveals the genetic heterogeneity of geleophysic dysplasia. PMID: 21415077
  6. These data suggest that ADAMTSL2 mutations may lead to a dysregulation of TGF-beta signaling and may be the underlying mechanism of geleophysic dysplasia. PMID: 18677313

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Involvement in disease
Geleophysic dysplasia 1 (GPHYSD1)
Subcellular Location
Secreted.
Database Links

HGNC: 14631

OMIM: 231050

KEGG: hsa:9719

STRING: 9606.ENSP00000346478

UniGene: Hs.522543

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