Human ADAMTS-like protein 2(ADAMTSL2) ELISA kit

Code CSB-EL001318HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ADAMTS-like 2
Alternative Names ADAMTS like 2 ELISA Kit; ADAMTS like protein 2 ELISA Kit; ADAMTS-like protein 2 ELISA Kit; ADAMTSL 2 ELISA Kit; ADAMTSL-2 ELISA Kit; ADAMTSL2 ELISA Kit; ATL2_HUMAN ELISA Kit; FLJ45164 ELISA Kit; KIAA0605 ELISA Kit
Abbreviation ADAMTSL2
Uniprot No. Q86TH1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAMTSL2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 99
Range % 94-106
1:2 Average % 88
Range % 84-93
1:4 Average % 92
Range % 87-98
1:8 Average % 102
Range % 98-106
The recovery of human ADAMTSL2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 87-96
EDTA plasma (n=4) 86 81-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 1.854 1.988 1.921 1.812
100 1.262 1.192 1.227 1.118
50 0.780 0.733 0.757 0.648
25 0.487 0.451 0.469 0.360
12.5 0.312 0.334 0.323 0.214
6.25 0.231 0.217 0.224 0.115
3.12 0.169 0.160 0.165 0.056
0 0.109 0.108 0.109  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Gene References into Functions
  1. CpG-specific DNA methylation of ADAMTSL2 and BTN3A2 at rheumatoid arthritis diagnosis can serve as a marker of treatment response. PMID: 28447857
  2. Two compound heterozygous mutations were confirmed in the ADAMTSL2 gene of the patient with geleophysic dysplasia. PMID: 28917829
  3. A novel mutation in ADAMTSL2 (p. Gly421Ser) gene was identified in individuals with Ehlers-Danlos Syndrome. PMID: 26879370
  4. Two novel homozygous missense mutations in the ADAMTSL2 gene underlie geleophysic dysplasia in two consanguineous families from the United Arab Emirates. PMID: 24014090
  5. Molecular screening of ADAMTSL2 gene in 33 patients reveals the genetic heterogeneity of geleophysic dysplasia. PMID: 21415077
  6. These data suggest that ADAMTSL2 mutations may lead to a dysregulation of TGF-beta signaling and may be the underlying mechanism of geleophysic dysplasia. PMID: 18677313

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Involvement in disease Geleophysic dysplasia 1 (GPHYSD1)
Subcellular Location Secreted
Database Links

HGNC: 14631

OMIM: 231050

KEGG: hsa:9719

STRING: 9606.ENSP00000346478

UniGene: Hs.522543


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