Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

Instructions
Code CSB-EL021850HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name sphingomyelin phosphodiesterase, acid-like 3B
Alternative Names SMPDL3B ELISA kit; ASML3B ELISA kit; ASMLPDAcid sphingomyelinase-like phosphodiesterase 3b ELISA kit; ASM-like phosphodiesterase 3b ELISA kit; EC 3.1.4.- ELISA kit
Abbreviation SMPDL3B
Uniprot No. Q92485
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SMPDL3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 83-89
1:2 Average % 92
Range % 89-97
1:4 Average % 100
Range % 95-105
1:8 Average % 90
Range % 87-93
Recovery
The recovery of human SMPDL3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-108
EDTA plasma (n=4) 99 95-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.243 2.102 2.173 2.031
20 1.545 1.632 1.589 1.447
10 1.149 1.201 1.175 1.033
5 0.825 0.835 0.830 0.688
2.5 0.505 0.531 0.518 0.376
1.25 0.345 0.362 0.354 0.212
0.625 0.224 0.215 0.220 0.078
0 0.144 0.140 0.142  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Lipid-modulating phosphodiesterase
Gene References into Functions
  1. Glomerular immunoreactivity and urinary excretion of SMPDL3b in proteinuric nephrotic syndrome patients decreased compared with controls. PMID: 29081073
  2. These results demonstrate that radiation injury induces early cytoskeletal remodeling, down-regulation of SMPDL3b, and elevation of cellular ceramide levels. Overexpression of SMPDL3b and pretreatment with rituximab confer a radioprotective effect in cultured podocytes. PMID: 27836988
Subcellular Location Secreted, Cell membrane, Lipid-anchor, GPI-anchor
Protein Families Acid sphingomyelinase family
Database Links

HGNC: 21416

OMIM: 617737

KEGG: hsa:27293

STRING: 9606.ENSP00000363001

UniGene: Hs.123659

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