Human Acid sphingomyelinase-like phosphodiesterase 3b(SMPDL3B) ELISA kit

Code CSB-EL021850HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
sphingomyelin phosphodiesterase, acid-like 3B
Alternative Names
SMPDL3B ELISA kit; ASML3B ELISA kit; ASMLPDAcid sphingomyelinase-like phosphodiesterase 3b ELISA kit; ASM-like phosphodiesterase 3b ELISA kit; EC 3.1.4.- ELISA kit
Abbreviation
SMPDL3B
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.625 ng/mL-40 ng/mL
Sensitivity
0.156 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SMPDL3B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 83-89
1:2 Average % 92
Range % 89-97
1:4 Average % 100
Range % 95-105
1:8 Average % 90
Range % 87-93
Recovery
The recovery of human SMPDL3B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 104 100-108
EDTA plasma (n=4) 99 95-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.243 2.102 2.173 2.031
20 1.545 1.632 1.589 1.447
10 1.149 1.201 1.175 1.033
5 0.825 0.835 0.830 0.688
2.5 0.505 0.531 0.518 0.376
1.25 0.345 0.362 0.354 0.212
0.625 0.224 0.215 0.220 0.078
0 0.144 0.140 0.142  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human SMPDL3B ELISA Kit was designed for the quantitative measurement of Human SMPDL3B protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

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Target Background

Function
(From Uniprot)
Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).
Gene References into Functions
  1. Glomerular immunoreactivity and urinary excretion of SMPDL3b in proteinuric nephrotic syndrome patients decreased compared with controls. PMID: 29081073
  2. These results demonstrate that radiation injury induces early cytoskeletal remodeling, down-regulation of SMPDL3b, and elevation of cellular ceramide levels. Overexpression of SMPDL3b and pretreatment with rituximab confer a radioprotective effect in cultured podocytes. PMID: 27836988
Subcellular Location
Secreted. Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Acid sphingomyelinase family
Database Links

HGNC: 21416

OMIM: 617737

KEGG: hsa:27293

STRING: 9606.ENSP00000363001

UniGene: Hs.123659

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