Human Angiotensin 1-7(Ang1-7)ELISA Kit

Code CSB-E14242h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Ang1-7
Target Name Angiotensin 1-7(Ang1-7
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %80-105
1:2Average %96
Range %85-109
1:4Average %105
Range %92-114
1:8Average %95
Range %82-110
Recovery
The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9082-110
EDTA plasma (n=4)9990-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.667 2.684 2.676 2.525
2501.934 1.939 1.937 1.786
1251.123 1.119 1.121 0.970
62.50.690 0.674 0.682 0.531
31.20.462 0.471 0.467 0.316
15.60.316 0.306 0.311 0.160
7.80.236 0.225 0.231 0.080
00.152 0.150 0.151  
References
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