Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Average % Recovery
EDTA plasma (n=4)
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
What are host animals of antibodies in the kit? Are the antibodies affinity purified?
Thanks for your inquiry.Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody. It is affinity purified. The standard is synthetic peptides. Pls let me know if you have any further questions. Thank you.