Rat angiotensin 1-7 (Ang1-7) ELISA kit

Instructions
Code CSB-E14241r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Angiotensin 1-7
Abbreviation Ang1-7
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 18.75 pg/mL-1200 pg/mL
Sensitivity 4.68 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %80-105
1:2Average %96
Range %85-104
1:4Average %102
Range %92-114
1:8Average %99
Range %80-110
Recovery
The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9683-112
EDTA plasma (n=4)9590-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12003.121 3.129 3.125 2.986
6002.532 2.522 2.527 2.388
3001.739 1.765 1.752 1.613
1501.050 1.041 1.046 0.907
750.623 0.642 0.633 0.494
37.50.435 0.447 0.441 0.302
18.750.265 0.254 0.260 0.121
00.135 0.143 0.139  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

What are host animals of antibodies in the kit? Are the antibodies affinity purified?

A:
Thanks for your inquiry.Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody. It is affinity purified. The standard is synthetic peptides. Pls let me know if you have any further questions. Thank you.

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