Rat angiotensin 1-7 (Ang1-7) ELISA kit

Code CSB-E14241r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Angiotensin 1-7
Abbreviation
Ang1-7
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
18.75 pg/mL-1200 pg/mL
Sensitivity
4.68 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %80-105
1:2Average %96
Range %85-104
1:4Average %102
Range %92-114
1:8Average %99
Range %80-110
Recovery
The recovery of rat Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9683-112
EDTA plasma (n=4)9590-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
12003.121 3.129 3.125 2.986
6002.532 2.522 2.527 2.388
3001.739 1.765 1.752 1.613
1501.050 1.041 1.046 0.907
750.623 0.642 0.633 0.494
37.50.435 0.447 0.441 0.302
18.750.265 0.254 0.260 0.121
00.135 0.143 0.139  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat Ang1-7 ELISA Kit was designed for the quantitative measurement of Rat Ang1-7 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.68 pg/mL.

Citations

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 Q&A
Q:

What are host animals of antibodies in the kit? Are the antibodies affinity purified?

A:
Thanks for your inquiry.Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody. It is affinity purified. The standard is synthetic peptides. Pls let me know if you have any further questions. Thank you.
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