Human Beta-klotho(KLB) ELISA kit

Code CSB-EL012378HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q86Z14
Lead Time 7-14 working days
Abbreviation KLB
Target Name klotho beta
Alias BKL, MGC142213, klotho beta like
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 15527
MGI 1932466
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human KLB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %102
Range %96-105
1:2Average %88
Range %84-92
1:4Average %94
Range %89-98
1:8Average %92
Range %88-94
Recovery
The recovery of human KLB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10296-106
EDTA plasma (n=4)8882-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.372 2.278 2.325 2.223
20001.746 1.844 1.795 1.693
10001.396 1.293 1.345 1.243
5000.972 0.961 0.967 0.865
2500.588 0.576 0.582 0.480
1250.354 0.368 0.361 0.259
62.50.225 0.230 0.228 0.126
00.103 0.101 0.102
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Function Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity).
Subcellular Location Cell membrane, Single-pass type III membrane protein
Protein Families Glycosyl hydrolase 1 family, Klotho subfamily
Database Links

HGNC: 15527

OMIM: 611135

KEGG: hsa:152831

STRING: 9606.ENSP00000257408

UniGene: Hs.90756

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