Human Bone morphogenetic protein 8B(BMP8B) ELISA kit

Code CSB-EL002746HU
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human BMP8B ELISA Kit was designed for the quantitative measurement of Human BMP8B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 9.37 pg/mL-600 pg/mL and the sensitivity is 2.34 pg/mL.

Target Name bone morphogenetic protein 8b
Alternative Names BMP-8 ELISA Kit; BMP-8B ELISA Kit; BMP8 ELISA Kit; Bmp8b ELISA Kit; BMP8B_HUMAN ELISA Kit; bone morphogenetic protein 8 (osteogenic protein 2) ELISA Kit; Bone morphogenetic protein 8 ELISA Kit; Bone morphogenetic protein 8B ELISA Kit; dJ118J21.1 (bone morphogenetic protein 8 (osteogenic protein 2)) ELISA Kit; OP-2 ELISA Kit; OP2 ELISA Kit; Osteogenic protein 2 ELISA Kit
Abbreviation BMP8B
Uniprot No. P34820
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 9.37 pg/mL-600 pg/mL
Sensitivity 2.34 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human BMP8B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 94  
Range % 90-98  
1:10 Average % 88  
Range % 82-94  
1:20 Average % 103  
Range % 99-108  
1:40 Average % 95  
Range % 90-100  
Recovery
The recovery of human BMP8B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-100  
EDTA plasma (n=4) 98 94-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
600 2.056 2.205 2.131 2.008  
300 1.607 1.724 1.666 1.543  
150 1.102 1.075 1.089 0.966  
75 0.645 0.603 0.624 0.501  
37.5 0.394 0.373 0.384 0.261  
18.75 0.291 0.302 0.297 0.174  
9.37 0.187 0.201 0.194 0.071  
0 0.120 0.125 0.123    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Gene References into Functions
  1. Acetylated H3K9 and H4K16 levels at BMP8B were increased in gastric cancer (GC) compared to nontumors (P < 0.05). However, reduced levels of acetylated H3K9 and H4K16 were associated with poorly differentiated GC (P < 0.05). PMID: 27748538
  2. Study found that BMP8B was downregulated in pancreatic cancer tissues and BMP8B mediates the survival of pancreatic cancer cells and regulates the progression of pancreatic cancer. PMID: 25176058
  3. Five novel SNPs within BMP8B gene.Genetic variants in BMP8B gene are associated with growth traits in Chinese native cattle. PMID: 24076131
  4. Epigenetic silencing of the bone morphogenetic protein 2 gene through methylation is associated with gastric carcinomas PMID: 16314833
  5. Reports on the mouse homolog of this gene. PMID: 9070944

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Subcellular Location Secreted.
Protein Families TGF-beta family
Database Links

HGNC: 1075

OMIM: 602284

KEGG: hsa:656

STRING: 9606.ENSP00000361915

UniGene: Hs.734367

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