Human C -type natriuretic peptide,CNP ELISA Kit

Instructions
Code CSB-E08909h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name natriuretic peptide precursor C
Alternative Names ANFC_HUMAN ELISA Kit; C type natriuretic peptide precursor ELISA Kit; CNP ELISA Kit; CNP-53 ELISA Kit; CNP2 ELISA Kit; Natriuretic peptide C ELISA Kit; Natriuretic peptide precursor C ELISA Kit; NPPC ELISA Kit
Abbreviation NPPC
Uniprot No. P23582
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays):CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 104    
Range % 92-108    
1:2 Average % 100    
Range % 92-104    
1:4 Average % 90    
Range % 83-94    
1:8 Average % 96    
Range % 88-100    
Recovery
The recovery of human CNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 90 81-94    
EDTA plasma (n=4) 107 97-112    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
4000 2.824 2.898 2.861 2.689    
2000 2.574 2.463 2.519 2.347    
1000 1.982 1.986 1.984 1.812    
500 1.163 1.192 1.178 1.006    
250 0.678 0.702 0.690 0.518    
125 0.572 0.561 0.567 0.395    
62.5 0.381 0.372 0.377 0.205    
0 0.174 0.170 0.172      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3 (By similarity).
Gene References into Functions
  1. two heterozygous NPPC mutations, located in the highly conserved ring, were identified; mutations cause autosomal dominant short stature in humans PMID: 28661490
  2. This study showed that Plasma concentrations of amino-terminal proCNP are depressed in Parkinson's Disease. PMID: 28673612
  3. C-type natriuretic peptide (CNP)administration significantly decreased the secretion and expression of MMP-2 and MMP-9 in the cultured mesangial cells ; (2) the secretion and expression of TIMP-1 progressively elevated after treatment with CNP for 24 and 48 h, whereas declined at later time point; (3) CNP expression was negatively correlated with MMP-2 and MMP-9 expression. PMID: 28554303
  4. CNP level in pericardial fluid is a more sensitive marker of LV dysfunction than CNP levels in plasma in coronary artery disease patients undergoing coronary artery bypass grafting. PMID: 26962968
  5. Data suggest mutations in NPR2 in patients with skeletal overgrowth alter conformation: A488P/R655C missense mutations yield conformation mimicking allosterically activated NPR2; A488P mutation sets phosphorylation as requirement for CNP-dependent activation; R655C mutation abrogates need for phosphorylation; ATP analog inhibits mutants. (NPR2 = atrial natriuretic factor receptor B; CNP = C-type natriuretic peptide) PMID: 28450398
  6. CNP may play a more important role than BNP and ANP related peptides, as risk marker of obesity PMID: 27376982
  7. CNP and FGF-23 act through a close or reciprocal pathway and are in agreement with recent studies demonstrating a down-regulatory role of the mitogen-activated protein kinase activity by CNP PMID: 26459742
  8. CNP/NPR-B can affect sperm motility and acrosome reaction, thus regulating the reproductive function of males PMID: 25926602
  9. CNP levels rose after paroxysmal supraventricular tachycardia and continued to rise after the termination. PMID: 26801306
  10. examined whether CNP reduces hyaluronan production and export via members of the multidrug resistance protein (MRP) and diminishes pro-inflammatory effects in chondrocytes PMID: 26307537
  11. Plasma CNP might serve as a useful predictor for the therapeutic efficacy of metoprolol on postural tachycardia syndrome in children. PMID: 25811760
  12. CNP/proCNP could be new markers in human prostate cancer PMID: 25808436
  13. This case supports the concept that NPPC is overexpressed because of the loss of a specific negative regulatory control in the normal chromosomal location, and demonstrates the effectiveness of targeted resequencing in the mapping of breakpoints. PMID: 25728306
  14. An increased level of NT-pro CNP is a promising non-invasive marker of hepatitis C related CLD complications and disease progression. PMID: 26485840
  15. CNP effectively attenuated LPS-induced endothelial activation by inhibiting the NF-kappaB and p38 signaling pathways. PMID: 25096521
  16. In achondroplasia, hypochondroplasia, and thanatophoric dysplasia, elevated plasma levels of proCNP products suggest the presence of tissue resistance to CNP. PMID: 25387261
  17. Data indicate an important, previously neglected, role of NEP for regulation of luminal factors in the epididymis and suggest a novel role for CNP/guanylyl cyclase B in the epididymal epithelium. PMID: 24099862
  18. This is the first report of a circulating preproCNP derived signal peptide. PMID: 24845564
  19. Gene expression of C-type natriuretic peptide and of its specific receptor NPR-B in human leukocytes of healthy and heart failure subjects PMID: 22884919
  20. C-type natriuretic peptide expression increases in human leukocytes of patients with chronic heart failure as a function of clinical disease severity. PMID: 23911666
  21. Acute inflammation in young children potently reduces CNP production. PMID: 23732776
  22. these observations suggest that CNP and its specific receptor, NPR-B, can play a very important role in regulating cardiac hypertrophy and remodeling, indicating NPR-B as a new potential drug target for the treatment of cardiovascular disease. PMID: 23262354
  23. DNA sequence analysis, single nucleotide polymorphism characterization and miRNA binding site identification in the 3'UTR of the human NPPC gene. PMID: 23542429
  24. CNP synthesis (as measured by NTproCNP levels in plasma) is closely related to linear growth in healthy children at all ages. We propose NTproCNP as a biomarker of linear growth. PMID: 22435455
  25. CNP production and clearance are increased in boys with Klinefelter syndrome. PMID: 22962429
  26. NPR2 expression in normal human fetal and adult pituitaries and adenomatous pituitary tissue and suggest a role for these receptors in both pituitary development and oncogenesis. PMID: 22645228
  27. Report the presence of CNP and its receptors, NPR2/3 in atherosclerotic plaques of human carotid artery. PMID: 22421372
  28. Serum NT-proCNP is elevated in advanced liver diseases and has prognostic value in cirrhotic patients. PMID: 22285383
  29. Continuous intravenous infusion of C-type natriuretic peptide did not attenuate the development of pulmonary hypertension caused by hypoxia and VEGF receptor blockade. PMID: 21820448
  30. The concentrations of amino-terminal pro-C-type natriuretic peptide in rheumatoid arthritis patients were significantly increased when compared to healthy controls. PMID: 20934962
  31. 56-year-old woman with Budd-Chiari syndrome who underwent liver transplantaation in whom heart failure was successfully managed using serial monitoring of BNP concentrations. PMID: 20832589
  32. The study indicates functional signaling by B- and C-type natriuretic peptides in vascular endothelial cells, supporting possible roles of these mediators in regulating endothelial cell function. PMID: 20085572
  33. Results support role of TSC22D1 as an enhancer of CNP transcription and suggest that TGF-beta-induced upregulation of CNP expression in smooth muscle may be mediated in part by increased transcription of TSC22D1. PMID: 20802130
  34. NT-proCNP was associated with the endosteal apposition of bone at the distal forearm in females with a persistent eating disorder PMID: 20551602
  35. Data suggest that decreased plasma proCNP concentration in idiopathic intracranial hypertension may reflect endothelial dysregulation of vascular tone and may be a marker in this disease. PMID: 20553977
  36. CNP/NPR-B signaling pathway is activated during TGF-beta1 induced chondrogenic differentiation of human trabecular bone-derived mesenchymal stem cells. PMID: 20721606
  37. new proCNP assay shows that proCNP is abundantly present in human seminal plasma and that seminal proCNP is secreted from the prostate gland and/or the seminal vesicles. PMID: 20797397
  38. Our data demonstrates that generation of CNP is not enhanced under heart failure condition like Chagas disease; CNP rise by severe HF is caused by its less degradation that is independent of neutral endopeptidase activity PMID: 20090473
  39. findings demonstrate that CNP metabolism is altered in patients with nephrotic syndrome and support the hypothesis that activation of renal CNP can be partially offset by a low-protein diet PMID: 12167597
  40. CNP G2628A polymorphism made an even greater contribution to hypertension in the younger subpopulation. PMID: 12452325
  41. human pro-CNP processing requires furin PMID: 12736257
  42. Particulate guanylate cyclase activation by CNP induces a post-transductional down-regulation of soluble guanylate cyclase by a mechanism involving PKG and the proteasome pathway PMID: 14654233
  43. The first intron in the CNP sequence does not contain any additional enhancer-binding sites. However, the signal sequence is indispensable for secretion of CNP and its appropriate physiological function. PMID: 15231517
  44. BNP levels appear to be elevated in coronary disease, especially in acute coronary syndromes, even in the absence of systolic dysfunction PMID: 15598085
  45. discussion of C-type natriuretic peptide and guanylyl cyclase B receptor [review] PMID: 15911070
  46. NT-proCNP may have a role in growth velocity and bone formation PMID: 16006435
  47. CNP is capable of autoregulating the expression of its cognate receptor. PMID: 16109786
  48. CNP is a modulator of acute inflammation in the blood vessel wall characterized by leukocyte and platelet activation, mediated, at least in part, via suppression of P-selectin expression. PMID: 16179391
  49. report suggests that in humans, platelet-derived growth factor(PDGF) and downstream activation of protein kinase C could represent an important control for natriuretic peptide precursor C expression in vascular smooth muscle PMID: 16777970
  50. There is a discordant expression of NPPC in newborn infants of mothers with type 1 diabetes. PMID: 17289171

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Subcellular Location Secreted
Protein Families Natriuretic peptide family
Database Links

HGNC: 7941

OMIM: 600296

KEGG: hsa:4880

STRING: 9606.ENSP00000295440

UniGene: Hs.247916

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