Human C-C motif chemokine 27 (CCL27) ELISA Kit

Code CSB-E09125h
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
C-C motif chemokine 27 (CCL27)
Alternative Names
ALP ELISA Kit; C-C motif chemokine 27 ELISA Kit; CC chemokine ILC ELISA Kit; Ccl27 ELISA Kit; CCL27_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 27 ELISA Kit; CTACK ELISA Kit; CTAK ELISA Kit; Cutaneous T cell attracting chemokine ELISA Kit; Cutaneous T-cell-attracting chemokine ELISA Kit; ESkine ELISA Kit; IL 11 R alpha locus chemokine ELISA Kit; IL-11 R-alpha-locus chemokine ELISA Kit; ILC ELISA Kit; PESKY ELISA Kit; SCYA27 ELISA Kit; Skinkine ELISA Kit; Small inducible cytokine A27 ELISA Kit; small inducible cytokine subfamily A (Cys-Cys); member 27 ELISA Kit; Small-inducible cytokine A27 ELISA Kit
Abbreviation
CCL27
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.313 ng/mL-20 ng/mL
Sensitivity
0.043 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CCL27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 85    
Range % 81-88    
1:2 Average % 95    
Range % 90-101    
1:4 Average % 104    
Range % 101-107    
1:8 Average % 89    
Range % 85-93    
Recovery
The recovery of human CCL27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 94-100    
EDTA plasma (n=4) 105 101-109    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
20 2.715 2.768 2.742 2.589    
10 2.097 2.014 2.056 1.903    
5 1.276 1.234 1.255 1.102    
2.5 0.752 0.768 0.760 0.607    
1.25 0.481 0.498 0.490 0.337    
0.625 0.327 0.310 0.319 0.166    
0.313 0.257 0.244 0.251 0.098    
0 0.156 0.150 0.153      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This human CCL27 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CCL27 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the CCL27 levels in the sample and is measured at 450 nm through a microplate reader.

CCL27 is an inflammatory chemokine mainly expressed by skin keratinocytes. It is associated with homing of memory T cells to sites of inflammation. CCR10 is the only known receptor for CCL27. Studies have shown that CCL27/CCR10 axis primarily functions in aiding the establishment of tissue-resident lymphocytes and maintenance of skin immune homeostasis. CCL27 expression is linked to normal skin development and keratinocyte differentiation. In healthy skin, CCL27 expression was predominantly detected in keratinocytes of the epidermis and hair follicles (HF) in both humans and mice. CCL27 expression is inhibited in the lesional skin of various skin inflammatory diseases such as psoriasis, hidradenitis suppurativa (HS), and alopecia areata. Kraynyak et al. have shown that CCL27 has adjuvant activity, enhancing immune responses to HIV-1 and SIV antigens.

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Target Background

Function
(From Uniprot)
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Gene References into Functions
  1. Plasma CCL27 levels were significantly lower in the VCA-IgA-negative normal subjects than in either the VCA-IgA-positive healthy donorsor the NPC patients. PMID: 29295705
  2. CCR10/CCL27 crosstalk mediated drug resistance, contributing to failure of proteasome-inhibitors in multiple myeloma. PMID: 27732933
  3. detected in the nucleus in eczema, cytoplasm in psoriasis PMID: 27193975
  4. CCL27 drives baseline recruitment of Herpes simplex virus-specific CD8 T cells expressing CCR10, while interferon-responsive CXCR3 ligands recruit additional cells in response to virus-driven inflammation. PMID: 28701399
  5. We therefore concluded that the cross talk between TNFa and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response. PMID: 27879026
  6. Our study showed that a higher immunostaining of CCL27 in supratumoral epidermis is associated with longer progression-free interval and melanoma-specific survival. PMID: 27325798
  7. Our data supports previous reports showing IL-17 and -23 upregulation in association with Multiple sclerosis and potentially identify a previously unknown involvement for CCL27. PMID: 26295034
  8. NOS2 and CCL27: clinical implications for psoriasis and eczema diagnosis and management. PMID: 25539641
  9. Findings support the notion that CCR10 and its ligand CCL27 may contribute to the skin infiltration of malignant T-cells in mycosis fungoides and adult T-cell leukemia/lymphoma. PMID: 24970722
  10. Our study suggests that CTACK/CCL27 may have a pivotal role in the early stage of psoriasis plaque formation, but should be downregulated in the later stage to induce inflammation characteristic for chronic psoriasis plaques PMID: 24710735
  11. Seventeen mediators were identified in burn wound exudates (concentration range 0.0006-9 ng/mg total protein), including the skin-specific chemokine CCL27. PMID: 23980822
  12. We show that the adult but not prenatal human keratinocytes produce and release large amounts of CCL27. PMID: 24037339
  13. CCL27 chemokine implicates cholesteatoma keratinocytes as contributors in cholesteatoma progression. PMID: 23670528
  14. low CCL27/CCR10 and CXCL12/CXCR4 intratumoral mRNA ratios are associated with melanoma progression PMID: 22526457
  15. Serum concentrations of TARC and CTACK were significantly higher in AD (atopic dermatitis) children than in healthy controls, and correlated with severity of symptoms. PMID: 22017510
  16. IL-1beta-induced CCL27 gene expression in normal human keratinocytes is regulated through the p38 MAPK/MSK1/Mnk1+2 as well as the IKKbeta/NF-kappaB signalling pathways. PMID: 21993219
  17. expression of CCL27 and CCL17 in the inflammatory skin diseases: psoriasis, atopic dermatitis and acute allergic contact dermatitis induced in nickel-sensitive individuals PMID: 21707761
  18. member of cytokine family; a chemokine PMID: 11821893
  19. membrane of cytokine family PMID: 11821900
  20. Serum CTACK levels in patients with atopic dermatitis(AD) and psoriasis vulgaris(pSv) were significantly higher. CTACK was strongly expressed in lesional ke-ratinocytes of patients with AD and PsV. PMID: 12642842
  21. serum levels significantly correlated with disease activity in patients with atopic dermatitis PMID: 14767451
  22. primary Th2-dominated inflammatory reaction in atopic dermatitis induced by TARC leads to an augmented skin-specific inflammatory reaction through CTACK. PMID: 15335355
  23. PSGL-1 interacts with CCL27 (CTACK/ILC/ESkine), a skin-associated chemokine that attracts skin-homing T lymphocytes PMID: 15466853
  24. CCL27 expression is under the control of NF-kappaB, and that NF-kappaB, as indicated by others, may be an attractive target for therapy in inflammatory skin diseases PMID: 15598438
  25. IL-1alpha, TNF-alpha, CCL20, CCL27, and CXCL8 alarm signals are induced in human cells after allergen and irritant exposure PMID: 15679580
  26. CCL28 produced by keratinocytes is mediated by different signal pathways from CCL27, and both CCL27 and CCL28 are involved in the pathogenesis of inflammatory skin diseases. PMID: 16433680
  27. CCR10-CTACK/CCL27 interactions between circulating T cells and keratinocytes would seem to play an important role in the pathophysiology of mycosis fungoides. PMID: 16675558
  28. LTB(4) may enhance TNF-alpha-induced CCL27 production by activating NF-kappaB via the BLT1/G(i/o)/PI3K/ERK pathway in human keratinocytes. PMID: 17581202
  29. Human keratinocyte-derived skin tumors may evade T cell-mediated antitumor immune responses by down-regulating the expression of CCL27 through the activation of epidermal growth factor receptor (EGFR)-Ras-MAPK-signaling pathways. PMID: 18025475
  30. Serum concentrations of CCL17, CCL22, and CCL27 correlate well with the extent and intensity of Atopic dermatitis (AD) in infants. Of the three Th2 chemokines examined, serum CCL27 correlated most significantly with the severity of AD PMID: 18266834
  31. a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis PMID: 18453562
  32. Significantly lower serum concentration of CXCL-9, CXCL-10, CCL-17, and IL-18 and higher concentration of CXCL-12 and CCL-27 were found in atopic dermatitis patients under 10 years old when compared to Control. PMID: 19639049

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Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Tissue Specificity
Testis, thymus, placenta, ovary and skin.
Database Links

HGNC: 10626

OMIM: 604833

KEGG: hsa:10850

STRING: 9606.ENSP00000259631

UniGene: Hs.648124

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