Product Details

Purity

>96% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CCR10 transfected BaF3 cells is in a concentration range of 10-100 ng/ml.

Target Names

CCL27

Uniprot No.

Q9Y4X3

Research Area

Immunology

Alternative Names

ALP; C-C motif chemokine 27; CC chemokine ILC; Ccl27; CCL27_HUMAN; Chemokine (C-C motif) ligand 27; CTACK; CTAK; Cutaneous T cell attracting chemokine; Cutaneous T-cell-attracting chemokine; ESkine; IL 11 R alpha locus chemokine; IL-11 R-alpha-locus chemokine; ILC; PESKY; SCYA27; Skinkine; Small inducible cytokine A27 ; small inducible cytokine subfamily A (Cys-Cys); member 27; Small-inducible cytokine A27

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

25-112aa

Complete Sequence

FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG

Mol. Weight

10.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL27 (CTACK) plays a specialized role in skin-homing T cell recruitment through its exclusive receptor CCR10, making it a critical tool for studying cutaneous immune responses. This tag-free recombinant human CCL27 encompasses the full-length mature protein (residues 25–112) and demonstrates confirmed chemotactic activity in CCR10-transfected BaF3 cell migration assays at 10–100 ng/ml, supporting its use in transwell chemotaxis experiments, skin-directed immune cell trafficking studies, and downstream CCR10 signaling pathway analyses including MAPK and NF-κB activation. The endotoxin level of less than 1.0 EU/μg satisfies the stringent criteria needed to prevent LPS-driven artifacts in sensitive immune cell functional assays, while also providing a suitable basis for in vivo inflammation models examining cutaneous T cell homing. Purity exceeding 96% by SDS-PAGE, combined with faithful mature-sequence fidelity from E. coli expression, makes this protein appropriate for use as a reference standard in chemokine detection assays and as a positive control in antibody validation workflows.

Target Background

Function

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.

Gene References into Functions

Plasma CCL27 levels were significantly lower in the VCA-IgA-negative normal subjects than in either the VCA-IgA-positive healthy donorsor the NPC patients. PMID: 29295705
CCR10/CCL27 crosstalk mediated drug resistance, contributing to failure of proteasome-inhibitors in multiple myeloma. PMID: 27732933
detected in the nucleus in eczema, cytoplasm in psoriasis PMID: 27193975
CCL27 drives baseline recruitment of Herpes simplex virus-specific CD8 T cells expressing CCR10, while interferon-responsive CXCR3 ligands recruit additional cells in response to virus-driven inflammation. PMID: 28701399
We therefore concluded that the cross talk between TNFa and ROS after keratinocytes was exposed to radiation, triggered CCL27 secretion for subsequent inflammation response. PMID: 27879026
Our study showed that a higher immunostaining of CCL27 in supratumoral epidermis is associated with longer progression-free interval and melanoma-specific survival. PMID: 27325798
Our data supports previous reports showing IL-17 and -23 upregulation in association with Multiple sclerosis and potentially identify a previously unknown involvement for CCL27. PMID: 26295034
NOS2 and CCL27: clinical implications for psoriasis and eczema diagnosis and management. PMID: 25539641
Findings support the notion that CCR10 and its ligand CCL27 may contribute to the skin infiltration of malignant T-cells in mycosis fungoides and adult T-cell leukemia/lymphoma. PMID: 24970722
Our study suggests that CTACK/CCL27 may have a pivotal role in the early stage of psoriasis plaque formation, but should be downregulated in the later stage to induce inflammation characteristic for chronic psoriasis plaques PMID: 24710735
Seventeen mediators were identified in burn wound exudates (concentration range 0.0006-9 ng/mg total protein), including the skin-specific chemokine CCL27. PMID: 23980822
We show that the adult but not prenatal human keratinocytes produce and release large amounts of CCL27. PMID: 24037339
CCL27 chemokine implicates cholesteatoma keratinocytes as contributors in cholesteatoma progression. PMID: 23670528
low CCL27/CCR10 and CXCL12/CXCR4 intratumoral mRNA ratios are associated with melanoma progression PMID: 22526457
Serum concentrations of TARC and CTACK were significantly higher in AD (atopic dermatitis) children than in healthy controls, and correlated with severity of symptoms. PMID: 22017510
IL-1beta-induced CCL27 gene expression in normal human keratinocytes is regulated through the p38 MAPK/MSK1/Mnk1+2 as well as the IKKbeta/NF-kappaB signalling pathways. PMID: 21993219
expression of CCL27 and CCL17 in the inflammatory skin diseases: psoriasis, atopic dermatitis and acute allergic contact dermatitis induced in nickel-sensitive individuals PMID: 21707761
member of cytokine family; a chemokine PMID: 11821893
membrane of cytokine family PMID: 11821900
Serum CTACK levels in patients with atopic dermatitis(AD) and psoriasis vulgaris(pSv) were significantly higher. CTACK was strongly expressed in lesional ke-ratinocytes of patients with AD and PsV. PMID: 12642842
serum levels significantly correlated with disease activity in patients with atopic dermatitis PMID: 14767451
primary Th2-dominated inflammatory reaction in atopic dermatitis induced by TARC leads to an augmented skin-specific inflammatory reaction through CTACK. PMID: 15335355
PSGL-1 interacts with CCL27 (CTACK/ILC/ESkine), a skin-associated chemokine that attracts skin-homing T lymphocytes PMID: 15466853
CCL27 expression is under the control of NF-kappaB, and that NF-kappaB, as indicated by others, may be an attractive target for therapy in inflammatory skin diseases PMID: 15598438
IL-1alpha, TNF-alpha, CCL20, CCL27, and CXCL8 alarm signals are induced in human cells after allergen and irritant exposure PMID: 15679580
CCL28 produced by keratinocytes is mediated by different signal pathways from CCL27, and both CCL27 and CCL28 are involved in the pathogenesis of inflammatory skin diseases. PMID: 16433680
CCR10-CTACK/CCL27 interactions between circulating T cells and keratinocytes would seem to play an important role in the pathophysiology of mycosis fungoides. PMID: 16675558
LTB(4) may enhance TNF-alpha-induced CCL27 production by activating NF-kappaB via the BLT1/G(i/o)/PI3K/ERK pathway in human keratinocytes. PMID: 17581202
Human keratinocyte-derived skin tumors may evade T cell-mediated antitumor immune responses by down-regulating the expression of CCL27 through the activation of epidermal growth factor receptor (EGFR)-Ras-MAPK-signaling pathways. PMID: 18025475
Serum concentrations of CCL17, CCL22, and CCL27 correlate well with the extent and intensity of Atopic dermatitis (AD) in infants. Of the three Th2 chemokines examined, serum CCL27 correlated most significantly with the severity of AD PMID: 18266834
a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis PMID: 18453562
Significantly lower serum concentration of CXCL-9, CXCL-10, CCL-17, and IL-18 and higher concentration of CXCL-12 and CCL-27 were found in atopic dermatitis patients under 10 years old when compared to Control. PMID: 19639049

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Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Tissue Specificity

Testis, thymus, placenta, ovary and skin.

Database Links

HGNC: 10626

OMIM: 604833

KEGG: hsa:10850

STRING: 9606.ENSP00000259631

UniGene: Hs.648124

Code	CSB-AP001001HU
Abbreviation	Recombinant Human CCL27 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human C-C motif chemokine 27 protein (CCL27) (Active)

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