Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

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Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

Protocol MSDS

Code	CSB-EL005899HU
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

carboxypeptidase N, polypeptide 2

Alternative Names

CPN2 ELISA kit; ACBPCarboxypeptidase N subunit 2 ELISA kit; Carboxypeptidase N 83 kDa chain ELISA kit; Carboxypeptidase N large subunit ELISA kit; Carboxypeptidase N polypeptide 2 ELISA kit; Carboxypeptidase N regulatory subunit ELISA kit

Abbreviation

CPN2

Uniprot No.

P22792

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CPN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-105
1:2	Average %	106
	Range %	102-112
1:4	Average %	97
	Range %	91-103
1:8	Average %	93
	Range %	89-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.858

2.934

2.896

2.755

2.131

2.167

2.149

2.008

2.5

1.275

1.328

1.302

1.160

1.25

0.837

0.829

0.833

0.692

0.625

0.492

0.521

0.506

0.365

0.312

0.317

0.323

0.320

0.179

0.156

0.238

0.244

0.241

0.100

0.137

0.145

0.141

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human CPN2 ELISA Kit was designed for the quantitative measurement of Human CPN2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Function
(From Uniprot)

The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.

Gene References into Functions

Real-time PCR and western blotting show the following CPN2 expression: minimal in MDA-MB-468 cells but obvious in MDA-MB-231 and MCF7 cells. MDA-MB-231 breast cancer cell lines were selected for the control group and CPN2 was knocked out for the experimental group. PMID: 26860443

Subcellular Location

Secreted.

Database Links

HGNC: 2313

OMIM: 603104

KEGG: hsa:1370

STRING: 9606.ENSP00000319464

UniGene: Hs.528368

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.