Human Cartilage intermediate layer protein 2(CILP2) ELISA kit

Code CSB-EL005438HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
cartilage intermediate layer protein 2
Alternative Names
CILP2 ELISA Kit; Cartilage intermediate layer protein 2 ELISA Kit; CILP-2) [Cleaved into: Cartilage intermediate layer protein 2 C1; Cartilage intermediate layer protein 2 C2] ELISA Kit
Abbreviation
CILP2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
25 pg/mL-1600 pg/mL
Sensitivity
6.25 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CILP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 98  
Range % 91-103  
1:2 Average % 96  
Range % 90-100  
1:4 Average % 85  
Range % 80-90  
1:8 Average % 92  
Range % 87-98  
Recovery
The recovery of human CILP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 84-93  
EDTA plasma (n=4) 90 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1600 2.153 1.984 2.069 1.986  
800 1.747 1.712 1.730 1.647  
400 1.387 1.301 1.344 1.261  
200 0.965 0.939 0.952 0.869  
100 0.643 0.604 0.624 0.541  
50 0.311 0.289 0.300 0.217  
25 0.192 0.179 0.186 0.103  
0 0.085 0.080 0.083    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CILP2 ELISA Kit was designed for the quantitative measurement of Human CILP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

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Target Background

Function
(From Uniprot)
May play a role in cartilage scaffolding.
Gene References into Functions
  1. This study aims to analyze the influences of single-nucleotide polymorphism in the NCAN-CILP2 region on non-alcoholic fatty liver disease and plasma lipid levels in the Asian and Pacific ethnic groups. PMID: 26758378
  2. The minor T allele of CILP2 gene and I allele of ACE gene have a protective effect. PMID: 24350279
  3. Cartilage intermediate layer protein 2 (CILP-2) is expressed in articular and meniscal cartilage and down-regulated in experimental osteoarthritis. PMID: 21880736
  4. Data show no differences in triglyceride or total cholesterol levels in relation to any allelic variants of ANGPTL3, CILP2, or TRIB1 SNPs. PMID: 21691831
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
Expressed in articular chondrocytes but not in knee meniscal cartilage cells. Localizes to the intermediate to deep zone of articular cartilage.
Database Links

HGNC: 24213

OMIM: 612419

KEGG: hsa:148113

STRING: 9606.ENSP00000291495

UniGene: Hs.279574

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