Human Caspase-9,Casp-9 ELISA Kit

Code CSB-E08862h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P55211
Lead Time 3-5 working days
Abbreviation CASP9
Protein Biological Process 1 Apoptosis/Autophagy
Target Name caspase 9, apoptosis-related cysteine peptidase
Alias RP11-265F14.3, APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cystein
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms.
HGNC 1511
RGD 61867
MGI 1277950
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %90
Range %84-94
1:2Average %96
Range %91-99
1:4Average %91
Range %87-94
1:8Average %100
Range %94-103
The recovery of human Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9588-97
EDTA plasma (n=4)10597-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
502.702 2.546 2.624 2.520
251.526 1.533 1.530 1.426
12.50.745 0.764 0.755 0.651
6.250.479 0.486 0.483 0.379
3.120.289 0.293 0.291 0.187
1.560.212 0.215 0.214 0.110
0.780.161 0.164 0.163 0.059
00.106 0.102 0.104
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Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; FUNCTION
Protein Families Peptidase C14A family
Tissue Specificity Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Database Links

HGNC: 1511

OMIM: 602234

KEGG: hsa:842

STRING: 9606.ENSP00000330237

UniGene: Hs.329502

Pathway p53 signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway

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