Human Caspase-9,Casp-9 ELISA Kit

Instructions
Code CSB-E08862h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name caspase 9, apoptosis-related cysteine peptidase
Alternative Names RP11-265F14.3, APAF-3, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9|caspase 9, apoptosis-related cystein
Abbreviation CASP9
Uniprot No. P55211
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Casp-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %84-94
1:2Average %96
Range %91-99
1:4Average %91
Range %87-94
1:8Average %100
Range %94-103
Recovery
The recovery of human Casp-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9588-97
EDTA plasma (n=4)10597-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.702 2.546 2.624 2.520
251.526 1.533 1.530 1.426
12.50.745 0.764 0.755 0.651
6.250.479 0.486 0.483 0.379
3.120.289 0.293 0.291 0.187
1.560.212 0.215 0.214 0.110
0.780.161 0.164 0.163 0.059
00.106 0.102 0.104
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; FUNCTION
Protein Families Peptidase C14A family
Tissue Specificity Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Database Links

HGNC: 1511

OMIM: 602234

KEGG: hsa:842

STRING: 9606.ENSP00000330237

UniGene: Hs.329502

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