Phospho-CASP9 (Thr125) Antibody

Datasheet
Code CSB-PA938158
Product Type Polyclonal Antibody
Size US$360
Uniprot No. P55211
Image
  • Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody.The lane on the right is treated with the antigen-specific peptide.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left)or the same antibody preincubated with blocking peptide (right).
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunogen Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).
Tested Applications ELISA,WB,IHC;WB:1:500-1:1000,IHC:1:50-1:100
Relevance

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Duan H., J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999)

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias APAF-3; MCH6; RNCASP9; CASP-9; ICE-LAP6
Immunogen Species Homo sapiens (Human)
Target Names CASP9
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; FUNCTION
Protein Families Peptidase C14A family
Tissue Specificity Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Database Links

HGNC: 1511

OMIM: 602234

KEGG: hsa:842

STRING: 9606.ENSP00000330237

UniGene: Hs.329502

Pathway p53 signaling pathway
PI3K-Akt signaling pathway
VEGF signaling pathway
Apoptosis

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