Human Catalase (CAT) ELISA kit

Instructions
Code CSB-E13635h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The product CSB-E13635h is a ready-to-use microwell, strip plate ELISA Kit for quantitatively analyzing the amount of the Catalase (CAT) in human serum, plasma, or tissue homogenates. This kit is designed for cancer research in humans. It has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which CAT in the samples or standards are sandwiched between pre-coated CAT antibody and Biotin-conjugated CAT antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of CAT bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of CAT in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

CAT is a common antioxidant enzyme found in nearly all living organisms exposed to oxygen and catalyzes the decomposition of hydrogen peroxide to water and oxygen. It plays an important role in protecting the cell from oxidative damage by reactive oxygen species (ROS). Deficiency or dysfunction of CAT has been regarded to be associated with the pathogenesis of various age-related degenerative diseases, such as Alzheimer's disease, Parkinson's disease, and cancer.

Target Name catalase
Alternative Names Cas1 ELISA Kit; CAT ELISA Kit; CATA_HUMAN ELISA Kit; Catalase ELISA Kit; Cs1 ELISA Kit; MGC138422 ELISA Kit; MGC138424 ELISA Kit
Abbreviation CAT
Uniprot No. P04040
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %86
Range %80-92
1:200Average %97
Range %91-105
1:400Average %99
Range %92-110
1:800Average %93
Range %86-98
Recovery
The recovery of human CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.420 2.333 2.377 2.282
5002.108 2.047 2.004 1.909
2501.582 1.555 1.569 1.474
1251.248 1.199 1.224 1.129
62.50.709 0.699 0.704 0.609
31.250.472 0.446 0.459 0.364
15.60.290 0.289 0.290 0.195
00.104 0.098 0.095  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CAT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled CAT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

May I know are the antibodies used in the ELISA kit polyclonal antibodies or monoclonal antibodies?
Human Catalase (CAT) ELISA kit
https://www.cusabio.com/ELISA-Kit/Human-Catalase-CAT-ELISA-kit-69103.html https://www.cusabio.com/ELISA-Kit/Human-Catalase-CAT-ELISA-kit-69103.html

A:
Thanks for your inquiry.Pls check the details as below:
Capture:mouse monoclonal antibody;Detection:mouse monoclonal antibody
If further questions,pls let me know.

Target Background

Function
(From Uniprot)
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Gene References into Functions
  1. 'T' allele of C262T showed a protective effect against the risk of ovarian cancer PMID: 29421935
  2. There was no significant association between the CAT gene SNP rs1001179 and Type 1 Diabetes or Diabetic Peripheral Neuropathy. PMID: 29924645
  3. Low CAT expression is associated with childhood obesity. PMID: 29953407
  4. CAT rs769218 GG was associated with poor overall survival compared to GA/AA genotypes in patients without platinum and fluorouracil-based adjuvant chemotherapy. PMID: 30114685
  5. homocysteine reacts with native catalase and/or redox-active transition metal ions to generate thiyl radicals that mediate compound II formation, a temporarily inactive state of the enzyme. PMID: 27848965
  6. Data suggest catalase (CAT) rs769214 (-844 G > A) polymorphism may be associated with N,N-dimethylacetamide (DMAc)-induced abnormal liver function in Chinese population. PMID: 28749186
  7. Maternal catalase activity was found to be lower in neural tube defect-affected pregnancies as compared to healthy controls. PMID: 28397206
  8. Catalase SNPs (C-262T and A-21T) show positive correlation in the model of SNP-SNP interaction in systemic lupus erythematosus. PMID: 28711952
  9. Study identifies an association of the polymorphism of CAT -262C/T in the Algerian population with type 1 diabetes without complications. PMID: 29859283
  10. Findings suggest that gene-environment interactions between the catalase polymorphism rs769214 and mold odor may play an important role in childhood allergic rhinitis development. PMID: 29028686
  11. catalase gene polymorphisms (rs1001179 and rs794316) have roles in cancer susceptibility [meta-analysis] PMID: 27449288
  12. the association between the different Catalase -262C/T genotypes and Catalase levels in seminal plasma and and male infertility PMID: 28436704
  13. This study showed that ADMA, carbonyl groups, CAT and NKA can be useful markers of chronic stress in both males and females with depression, PTSD, and depression concurrent with PTSD. PMID: 28554099
  14. The investigated MnSOD and Catalase polymorphisms do not predispose to the development of alcoholic Chronic Pancreatitis. PMID: 28655148
  15. the CAT C262T polymorphism may be a candidate markerfor cancer risk with type-specific and population-specific effects but not a fine prognostic factor for cancer survival. [meta-analysis] PMID: 27225983
  16. The CAT1 haplotype could be implicated in the level of metabolic and cardiovascular improvement after bariatric surgery. PMID: 29496557
  17. This study showed that CAT functional SNP rs769217 was significantly associated with primary open angle glaucoma (POAG), implying that the CAT gene variants may play a role in the pathogenesis of POAG in the Chinese population. PMID: 28829657
  18. By dividing patients into two groups, increased (P-I/R-I) and decreased TBARS (P-II/R-II) at T12 compared to T0, we found an increased CAT activity in conditioned endothelial cells at T12 in both groups (p=0.05 and p=0.001, respectively). PMID: 28652427
  19. research demonstrated that erysipelas infection predisposition and its clinical characteristics are affected by age, sex and SNPs found in SOD1, SOD2, and catalase genes; presence of SOD1 G7958, SOD2 T2734, and CAT C262 alleles was linked to erysipelas' predisposition PMID: 28512644
  20. A melanoma cell model with low levels of H2O2 induced by catalase overexpression to study differentiation/dedifferentiation processes. PMID: 27206672
  21. Gene expression microarrays in A375 melanoma cells with different ROS levels after overexpressing catalase were performed. Gene expression signature could be relevant in clinical prognostic to classify between non aggressive and metastatic melanomas. PMID: 27206673
  22. It has been shown that the AP-1 family member JunB and retinoic acid receptor alpha (RARa) mediate catalase transcriptional activation and repression, respectively, by controlling chromatin remodeling through a histone deacetylases-dependent mechanism. PMID: 27591797
  23. these findings lend credit to the idea that inefficient catalase import, when coupled with the role of PEX5 as a redox-regulated import receptor, constitutes a cellular defense mechanism to combat oxidative insults of extra-peroxisomal origin. PMID: 28760655
  24. our data suggest that gene polymorphisms of GPX1 Pro198Leu and CAT C262T may have a protective role in the development of primary open-angle glaucoma in a Polish population. PMID: 28547970
  25. Mutated obese carries of SOD1 -251, SOD2 47 and CAT -262 are associated with a higher distribution of fat in comparison with obese wild-type carriers. PMID: 27751366
  26. The present study determined that ARE, CLP, CAT, and MPO levels are different between the pediatric patients with sepsis and healthy controls. ARE level can be a potent biomarker for sepsis in critical patients in intensive care units. PMID: 28167245
  27. Conducted our audit should be extended to more group of patients in order to assess whether or not to confirm the observed during analysis, the protective effect of CAT-262 C / T in ulcerative colitis and other trends observed for other polymorphic variants tested genes. PMID: 28141554
  28. Superoxide dismutase and catalase activities were lower in type 1 myotonic dystrophy patients compared to healthy controls PMID: 26817806
  29. results suggest that, in dyslipidemic patients, the catalase rs1001179 TT genotype is associated with higher HbA1c and plasma triglyceride levels, and that these associations may be modulated by BMI and/or age PMID: 27704307
  30. Study demonstrates that SOD2 rs4880, GPX1 rs1050450 and CAT rs1001179 are not associated with an increased susceptibility to epilepsy after neonatal hypoxic-ischemic encephalopathyor its drug resistance PMID: 28222320
  31. The antioxidative effects of the Mediterranean diet against breast cancer risk may be enhanced by the wild-type alleles of the MnSOD or CAT SNPs among Greek-Cypriot women. PMID: 26130326
  32. The TT genotype distribution for the A/T polymorphism in catalase gene at position -21 was statistically significant differences in eosinophilic Chronic rhinosinusitis with nasal polyps and control groups. PMID: 27515707
  33. Genetic polymorphisms in eNOS, catalase and myeloperoxidase and their significance in a cohort of Turkish prostate cancer patients. PMID: 27706591
  34. Data show that selective and gradual inhibition of membrane-associated catalase of tumor cells or siRNA-mediated knockdown of the total catalase in tumor cells leads to an optimum curve of apoptosis induction. PMID: 28179303
  35. There were significant differences between catalase polymorphisms. The studied polymorphisms showed linkage disequilibrium. The mRNA levels of CAT in the AC/TT, TC/TC, TC/TT, and TC/TC diplotypes significantly were higher than the mRNA levels in AC/AC diplotype. PMID: 27225276
  36. CAT -844G>A (rs769214) polymorphism is associated with the development of Essential Hypertension in smokers. PMID: 27735913
  37. Study showed that endothelial cells from human umbilical vein have a reduced activity and gene expression of the "classic" antioxidant enzymes (Cu,Zn-superoxide dismutase, catalase, and Se-containing glutathione peroxidase). At the same time, a high expression level of peroxiredoxin genes was identified in the same endothelial cells PMID: 28058676
  38. The effects of oleuropein (OL) on hydrogen peroxide-induced oxidative stress in L02 cells showed that SOD1, GPx1, and catalase levels were all increased, and suggested that OL is a potent antioxidant and may be therapeutically useful in liver disease prevention. PMID: 27914828
  39. There were significant differences in the TT genotype distribution of the -21A>T polymorphism in the CAT gene between the tympanosclerosis and control groups, and between the chronic otitis media and control groups. PMID: 27534583
  40. The purpose of the study was to evaluate the plasma level of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) and the association between polymorphic variants in genes encoding for GPx1, SOD, CAT and the risk of distal symmetric polyneuropathy in type 2 diabetes mellitus patients. PMID: 26674569
  41. Among acute kidney injury patients in the Turkish population, hospital morbidity and mortality were found to be more frequent in patients bearing a T allele of the rs769217 polymorphism of the CAT gene. PMID: 26787049
  42. This study indicates that catalase C-262T polymorphism is associated with the endometriosis. PMID: 25154023
  43. Our results suggest that 389C>T may not contribute to vitiligo susceptibility. PMID: 25139332
  44. CAT rs1001179 polymorphism could be used to identify children that have a higher susceptibility to cerebral palsy after perinatal Hypoxic-ischaemic perinatal brain injury PMID: 27302388
  45. Increasing the endogenous NO level causes catalase inactivation and reactivation of intercellular apoptosis signaling specifically in tumor cells. PMID: 26342455
  46. In patients with chronic hepatitis C, the GPX1 Pro198Leu polymorphism, alone or combined with the CAT C-262T, was associated with high risk of fibrosis severity and hepatocellular carcinoma (HCC). In addition, GPX1 polymorphism was also associated with advanced stages of HCC. PMID: 26990426
  47. Deciphering the molecular mechanisms that regulate catalase expression could, therefore, be of crucial importance for the future development of pro-oxidant cancer chemotherapy. PMID: 26117330
  48. Study shows that the SOD2 16C/T and CAT -21A/T alleles may serve as useful genetic susceptibility markers for migraine PMID: 25818327
  49. the ratios SOD2/catalase and SOD2/Gpx1 could be considered as potential markers during progression from tumor growth to metastasis PMID: 25866291
  50. This study identified CAT is a Noise-induced hearing loss susceptibility gene when noise exposure levels are taken into account. PMID: 26045794

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Involvement in disease Acatalasemia (ACATLAS)
Subcellular Location Peroxisome
Protein Families Catalase family
Database Links

HGNC: 1516

OMIM: 115500

KEGG: hsa:847

STRING: 9606.ENSP00000241052

UniGene: Hs.502302

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