Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

Code CSB-EL004476HU
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cathelicidin antimicrobial peptide
Alternative Names CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37,
Abbreviation CAMP
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.45 ng/mL-30 ng/mL
Sensitivity 0.11 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human CAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 88  
Range % 80-92  
1:200 Average % 96  
Range % 91-105  
1:400 Average % 99  
Range % 92-110  
1:800 Average % 95  
Range % 86-98  
The recovery of human CAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 89-98  
EDTA plasma (n=4) 95 90-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
30 2.713 2.664 2.689 2.612  
15 1.687 1.567 1.627 1.550  
7.5 1.047 0.989 1.018 0.941  
3.75 0.563 0.543 0.553 0.476  
1.8 0.339 0.324 0.332 0.255  
0.9 0.206 0.196 0.201 0.124  
0.45 0.145 0.139 0.142 0.065  
0 0.080 0.074 0.077    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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we are interested in measurements of the Cathelicidin antimicrobial peptide (CAMP) in human fecal samples. According to your homepage, your assay is suitable for blood samples or tissue homogenates. But have you ever tested it in fecal samples? Would you expect it to work using fecal samples?

Thanks for your inquiry!
We tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test human fecal samplee ,we suggest you do a pretest . Pls find following sample preparation method.
Feces Dilute samples with appropriate Sample Diluent. This can be achieved by adding 0.1g sample to appropriate Sample Diluent. Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.
Pls let me know if you have any further questions. Thank you.

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