Human Cementum Protein 1 (CEMP-1) ELISA Kit

Instructions
Code CSB-E13012h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cementum protein 1
Alternative Names Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit
Abbreviation CEMP1
Uniprot No. Q6PRD7
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-95
1:2Average %101
Range %97-105
1:4Average %83
Range %80-86
1:8Average %107
Range %103-110
Recovery
The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)9184-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.658 2.697 2.678 2.528
52.312 2.331 2.322 2.172
2.51.871 1.892 1.882 1.732
1.251.312 1.365 1.339 1.189
0.6250.788 0.797 0.793 0.643
0.3120.442 0.472 0.457 0.307
0.1560.281 0.272 0.277 0.127
00.151 0.149 0.150
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function May play a role in development of the periodontium which surrounds and supports the teeth by promoting the differentiation of multi-potent cells from the periodontal ligament into cementoblasts to form the cementum
Gene References into Functions
  1. The synthesis of hydroxyapatite with different crystallinities by controlling the concentration of recombinant CEMP1 for biological application. PMID: 26652387
  2. CEMP1 exerts modulation of a number of cellular genes. PMID: 26011628
  3. when HIF-1 was activated by gene introduction or chemically, CEMP1 expression and mineralization increased. PMID: 24117017
  4. human primary cementoblasts subjected to compression and IL-1beta stimulation impeded BSP and CEMP-1 expression, proteins that are associated with cementogenesis. PMID: 22349547
  5. These data demonstrate for the first time that the CEMP1 is not only a marker protein for cementoblast-related cells, but it also regulates cementoblast commitment in periodontal ligament cells PMID: 21465469
  6. CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and it might have a potential function in cementum and bone formation PMID: 17509525
  7. CEMP1 plays a role during the biomineralization process by promoting octacalcium phosphate crystal nucleation. PMID: 19393626

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Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Expressed by cementoblasts, a subpopulation of periodontal ligament cells and cells located around vessels in periodontium (at protein level).
Database Links

HGNC: 32553

OMIM: 611113

KEGG: hsa:752014

STRING: 9606.ENSP00000371787

UniGene: Hs.433499

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