Human Cementum Protein 1 (CEMP-1) ELISA Kit

Code CSB-E13012h
Size 96T,5×96T,10×96T
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Product Details

Target Name
cementum protein 1
Alternative Names
Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit
Abbreviation
CEMP1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %85-95
1:2Average %101
Range %97-105
1:4Average %83
Range %80-86
1:8Average %107
Range %103-110
Recovery
The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9287-96
EDTA plasma (n=4)9184-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.658 2.697 2.678 2.528
52.312 2.331 2.322 2.172
2.51.871 1.892 1.882 1.732
1.251.312 1.365 1.339 1.189
0.6250.788 0.797 0.793 0.643
0.3120.442 0.472 0.457 0.307
0.1560.281 0.272 0.277 0.127
00.151 0.149 0.150
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CEMP1 ELISA Kit was designed for the quantitative measurement of Human CEMP1 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
May play a role in development of the periodontium which surrounds and supports the teeth by promoting the differentiation of multi-potent cells from the periodontal ligament into cementoblasts to form the cementum. Binds hydroxyapatite and may promote the biomineralization of the cementum. Also promotes cell proliferation.
Gene References into Functions
  1. The synthesis of hydroxyapatite with different crystallinities by controlling the concentration of recombinant CEMP1 for biological application. PMID: 26652387
  2. CEMP1 exerts modulation of a number of cellular genes. PMID: 26011628
  3. when HIF-1 was activated by gene introduction or chemically, CEMP1 expression and mineralization increased. PMID: 24117017
  4. human primary cementoblasts subjected to compression and IL-1beta stimulation impeded BSP and CEMP-1 expression, proteins that are associated with cementogenesis. PMID: 22349547
  5. These data demonstrate for the first time that the CEMP1 is not only a marker protein for cementoblast-related cells, but it also regulates cementoblast commitment in periodontal ligament cells PMID: 21465469
  6. CEMP1 might participate in differentiation and mineralization of nonosteogenic cells, and it might have a potential function in cementum and bone formation PMID: 17509525
  7. CEMP1 plays a role during the biomineralization process by promoting octacalcium phosphate crystal nucleation. PMID: 19393626

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Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Expressed by cementoblasts, a subpopulation of periodontal ligament cells and cells located around vessels in periodontium (at protein level).
Database Links

HGNC: 32553

OMIM: 611113

KEGG: hsa:752014

STRING: 9606.ENSP00000371787

UniGene: Hs.433499

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