Name: Human Chitinase-3-like Protein 1(YKL-40/CHI3L1)ELISA Kit
Brand: CUSABIO
SKU: CSB-E13608h

Product Details

Target Name

chitinase 3-like 1 (cartilage glycoprotein-39)

Alternative Names

39 kDa synovial protein ELISA Kit; ASRT7 ELISA Kit; Cartilage glycoprotein 39 ELISA Kit; CGP-39 ELISA Kit; CGP39 ELISA Kit; CH3L1_HUMAN ELISA Kit; CHI3L1 ELISA Kit; chitinase 3 like 1 (cartilage glycoprotein 39) ELISA Kit; chitinase 3 like 1 ELISA Kit; Chitinase 3 like protein 1 precursor ELISA Kit; chitinase ELISA Kit; Chitinase-3-like protein 1 ELISA Kit; Chondrocyte protein YKL40 ELISA Kit; GP 39 ELISA Kit; GP-39 ELISA Kit; GP39 ELISA Kit; HC gp39 ELISA Kit; HCGP 3P ELISA Kit; hCGP-39 ELISA Kit; HCgp39 ELISA Kit; YKL 40 ELISA Kit; YKL-40 ELISA Kit; YKL40 ELISA Kit; YYL 40 ELISA Kit

Abbreviation

CHI3L1

Uniprot No.

P36222

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

46.875 pg/mL-3000 pg/mL

Sensitivity

78.065 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Chitinase-3-like Protein 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human CHI3L1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CHI3L1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

CUSABIO’s Human YKL-40/CHI3L1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CHI3L1 in serum, plasma, and tissue homogenates. This assay employs the quantitative sandwich enzyme immunoassay technique, in which CHI3L1 in the samples or standards are sandwiched between pre-coated CHI3L1 antibody and HRP-conjugated antibody specific for CHI3L1. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of CHI3L1 bound in the initial step. The color development is stopped and the intensity of the color is measured at 450 nm via a microplate reader. This kit displays many advantages, including sensitivity, specificity, precision, linearity, and recovery. The product instructions are access to more information.

CHI3L1 is a secreted glycoprotein and prototypic mammalian chitinase-like protein belonging to the glycoside hydrolase family 18. It is synthesized and secreted by numerous cells such as macrophages, neutrophils, and fibroblast-like cells. It is mainly involved in tissue damage, tissue repair, inflammation, and remodeling responses. CHI3L1 has been closely related to diseases including asthma, arthritis, sepsis, diabetes, liver fibrosis, and coronary artery disease. Several solid tumors including breast, colon, kidney, ovarian, prostate, and glioblastoma, have detected the presence of CHI3L1. CHI3L1 signaling plays a critical role in cancer cell growth, proliferation, invasion, metastasis, angiogenesis, activation of tumor-associated macrophages, and Th2 polarization of CD4+ T cells. Elevated CHI3L1 levels are correlated with poor prognosis and decreased survival rates of cancer patients.

Target Background

Function
(From Uniprot)

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

Gene References into Functions

YKL-40 elevation significantly improved risk prediction for all-cause and cardiovascular mortality in hemodialysis patients. PMID: 28941940
Polymorphisms of the CHI3L1 gene (rs4950928 C>G and rs10399931 C>T) are associated with the risk and prognosis of AD and can affect the expression of CHI3L1 in plasma. PMID: 30223258
High YKL-40 expression is associated with endothelial cell angiogenesis in breast cancer. PMID: 29274508
Pretreatment serum levels of YKL-40 may be a novel prognostic factor of overall and disease-free survival in patients with nonmetastatic colorectal cancer PMID: 29642772
Plasma YKL-40 distinguishes between patients with features of asthma-chronic obstructive pulmonary disorder (COPD) overlap (ACO) and COPD patients. PMID: 29580282
CHI3L1 expression is a novel biomarker for the prognosis of gastric cancer and these findings have thus identified CHI3L1/CD44 axis as a vital pathway and potential therapeutic target in gastric cancer PMID: 30165890
YKL-40 was highly elevated in patients with ESRD on HD, and dialysis reduced serum YKL-40 concentrations approximately one-sixth. YKL-40 measured after dialysis was independently associated with mortality in HD patients. PMID: 29357700
Review/Meta-analysis: YKL-40 may be implicated in bronchial inflammation and remodeling in COPD and may be considered as a useful biomarker for COPD diagnosis and monitoring. PMID: 29430175
YKL-40 is a glycoprotein - a potential marker for active inflammatory process - with proven predictive value in a number of diseases that evolve with inflammation, remodeling of the extracellular matrix, or development of fibrosis. PMID: 29400369
TMEM219 plays a critical role in Chi3l1-induced IL-13Ralpha2 mediated signalling and tissue responses. PMID: 27629921
Our present data reveal the crucial role of CHI3L1 in the formation of vasculogenic mimicry, which may contribute to tumour aggressiveness of cervical cancer PMID: 29452694
there was a negative correlation between YKL-40 and TLR4 expression in chronic sinusitis patients with nasal polyps. YKL-40 and TLR4 interacted with each other to activate NF-kappaB and promote disease progression. PMID: 29921378
CHI3L1 may be a previously unrecognized biomarker for diagnosing Gaucher Diseases (GD) and for evaluating the therapeutic effects of new GD drug. PMID: 29396296
Study established a connection between the pretreated CHI3L1 and patients with esophageal squamous cell carcinoma (ESCC), and the serum CHI3L1 was primarily secreted by ESCC-surrounded macrophages. PMID: 29209110
YKL-40 is expressed in cutaneous Squamous Cell Skin Cancer PMID: 30061245
The G allele of CHI3L1 rs4950928 might be a protective factor against the development of asthma. PMID: 29233108
This study suggests that CHI3L1 and CHI3L2 are associated with the progression of neurodegeneration in motor cortex and spinal cord of Amyotrophic Lateral Sclerosis patients. PMID: 28989002
in neurodegenerative dementias, YKL-40 is a disease-specific marker of neuroinflammation showing its highest levels in prion diseases PMID: 29126445
Serum IL-6, serum YKL-40, and plasma VEGF were significantly correlated with disease activity at baseline in early rheumatoid arthritis. PMID: 29336711
high serum YKL-40 levels correlated with the severity of obstructive sleep apnea syndrome and might serve as a nonspecific biomarker for prediction and progression of the disease. PMID: 29028081
YKL-40 could be considered as a useful biomarker of inflammation in psoriasis and is more sensitive than CRP or WBC. Increased YKL-40 may indicate psoriatic patients with a higher level of systemic inflammation, which may determine disease management. PMID: 28932021
YKL-40 protein expression associated with severe lung function impairment and severe exacerbations in Asthma patients. PMID: 29025889
Results suggest a potential role of chitinase 3 like 1 protein (CHI3L1) in angiogenesis in invasive ductal breast carcinoma (IDC) and may suggest its involvement in cancer progression. PMID: 29848684
YKL-40 is a promising biomarker for evaluating polymyositis/dermatomyositis-associated interstitial lung disease activity/severity and predicting disease prognosis. PMID: 28711881
Early-onset atopic dermatitis was associated with rs2303067 in SPINK5, which is involved in skin barrier functioning, and late-onset was associated with rs4950928 in CHI3L1, which is involved in the immune response. Future studies should examine the early- versus late-onset subgrouping more closely. PMID: 28681574
YKL-40 may represent a novel biomarker for predicting hypertension risk in prehypertension population PMID: 28797750
A high diagnostic sensitivity of YKL-40 in the early stages of the disease suggests the possibility of using this biomarker at an early diagnostic phase of patients with endometrial cancer. The patients with increased levels of YKL-40 before treatment are also at the higher risk of relapse. PMID: 28624692
Serum YKL-40 was elevated only in atrial fibrllation and not in other supraventricular arrhythmias. PMID: 28639986
YKL-40 is elevated in serum and bronchoalveolar lavage fluid of pulmonary alveolar proteinosis (PAP) patients, and may be of clinical utility to predict outcome in PAP PMID: 28569052
strongly expressed in placenta percreta and correlated with extravillous trophoblast invasion PMID: 27977409
YKL-40 was expressed by macrophages in liver tissue of NAFLD patients. PMID: 27739482
The results of this study indicate that YKL-40 may contribute to decreased stability and increased permeability of BBB in AD patients PMID: 28697565
YKL-40 is expressed by a subset of astrocytes in Alzheimer's disease and other tauopathies. PMID: 28599675
This study has shown that YKL-40 serum level is increased in patients with atopic dermatitis and reflects the severity of symptoms. PMID: 28660216
YKL-40 levels were found to be significantly higher in post-infectious bronchiolitis obliterans (PIBO) patients with exacerbation compared with that in bronchiolitis patients and showed a positive correlation with the severity of disease before diagnosis of PIBO. PMID: 28567534
miR-24 serves as a molecular regulator in S. aureus-induced macrophage polarization through targeting of CHI3L1 and regulation of the MAPK pathway PMID: 28303416
High YKL-40 expression is independently and markedly associated with worse overall survival in glioblastoma patients. PMID: 27090900
Datashow that patients with YKL-40 overexpression had significantly shorter survival time than those with low YKL-40 expression. PMID: 28099248
The level of serum YKL-40 is a significant and independent biomarker to predict the clinical outcome in large-artery atherosclerotic stroke. PMID: 27650381
TrkB-containing exosomes play a key role in the control of glioblastoma progression and aggressiveness, as seen in a model of YKL-40-inactivated glioblastoma cells PMID: 27385098
findings suggest that these pulmonary markers could be useful to assess CAP severity and, especially YKL-40 and CCL18 by helping predict CAP caused by atypical pathogens PMID: 29324810
The present meta-analysis indicate that elevated YKL-40 expression is associated with a poor prognosis in breast cancer patients. YKL-40 may serve as a promising predictive biomarker of prognosis of breast cancer PMID: 28036271
YKL-40 induces IL-18 production in osteoblasts and thereby stimulates angiogenesis of endothelial progenitor cells through the suppression of miR-590-3p via the focal adhesion kinase (FAK)/PI3K/Akt signaling pathway. PMID: 28448439
YKL-40 levels showed a positive correlation with blood neutrophil counts but no consistent relationships with clinical characteristics of relevance to continuous wheeze in preschool children PMID: 27732738
Study suggests that YKL-40 is associated with hypertension incidence only among men. PMID: 27815265
In HCT donors with YKL-40 plasma concentrations, a trend toward increased grade II-IV acute GvHD in recipients was observed with no significant associations with overall survival, treatment-related mortality or relapse PMID: 27427920
Review of evidence for the role of YKL-40 in the diagnosis, prognosis and cause of cardiovascular and alcoholic liver disease. PMID: 27187575
the level of CHI3L1 protein in the sera of patients with gastric or breast cancer was significantly elevated compared with those of healthy donors. M2 macrophage-secreted CHI3L1 promoted the metastasis of gastric and breast cancer cells in vitro and in vivo PMID: 28143526
Besides being associated with tau pathology, CSF YKL-40 adds to the growing array of biomarkers reflecting distinct molecular brain mechanisms potentially useful for stratifying individuals for biomarker-guided, targeted anti-inflammatory therapies emerging from precision medicine. PMID: 28281838
SNPs in CHI3L1 and cord blood YKL-40 were not associated with lung function measurements at 5 weeks and 6 years, respiratory symptoms in the first year, and allergic sensitisation at 6 years. Genetic variation in CHI3L1 might be related to the development of milder forms of asthma. PMID: 27193312

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Involvement in disease

Asthma-related traits 7 (ASRT7); Schizophrenia (SCZD)

Subcellular Location

Secreted, extracellular space. Cytoplasm. Cytoplasm, perinuclear region. Endoplasmic reticulum.

Protein Families

Glycosyl hydrolase 18 family

Tissue Specificity

Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

Database Links

HGNC: 1932

OMIM: 181500

KEGG: hsa:1116

STRING: 9606.ENSP00000255409

UniGene: Hs.382202

Code	CSB-E13608h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Human Chitinase-3-like Protein 1(YKL-40/CHI3L1)ELISA Kit

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