Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP-39; CGP39; CH3L1_HUMAN; CHI3L1; chitinase 3 like 1 (cartilage glycoprotein 39) ; chitinase 3 like 1; Chitinase 3 like protein 1 precursor ; chitinase; Chitinase-3-like protein 1; Chondrocyte protein YKL40; GP 39; GP-39; GP39; HC gp39 ; HCGP 3P ; hCGP-39; HCgp39; YKL 40; YKL-40; YKL40; YYL 40
Species
Homo sapiens (Human)
Expression Region
22-383aa
Target Protein Sequence
YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 22-383 form the expressed segment for recombinant Human CHI3L1. This CHI3L1 protein is expected to have a theoretical molecular weight of 60.5 kDa. This CHI3L1 protein is produced using e.coli expression system. The N-terminal 10xHis-SUMO tag and C-terminal Myc tag was fused into the coding gene segment of CHI3L1, making it easier to detect and purify the CHI3L1 recombinant protein in the later stages of expression and purification.
Chitinase-3-like protein 1 (CHI3L1) is a multifaceted protein with a primary focus in the areas of immunology and inflammation. Its most crucial role is observed in the study of inflammatory processes, where CHI3L1 serves as a biomarker and mediator. CHI3L1 is associated with chronic inflammatory conditions and has become a key player in research related to autoimmune diseases and allergic reactions. In immunology, CHI3L1 is actively investigated for its involvement in modulating immune responses. It plays a role in regulating the activity of immune cells and contributes to the intricate network of signaling pathways that orchestrate inflammation. Furthermore, CHI3L1 is gaining attention in cancer research, particularly in exploring its potential as a diagnostic or prognostic marker. Studies have indicated elevated levels of CHI3L1 in certain cancers, and its association with tumor progression is an area of active investigation.