Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

CHI3L1

Uniprot No.

P36222

Research Area

Signal Transduction

Alternative Names

39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP-39; CGP39; CH3L1_HUMAN; CHI3L1; chitinase 3 like 1 (cartilage glycoprotein 39) ; chitinase 3 like 1; Chitinase 3 like protein 1 precursor ; chitinase; Chitinase-3-like protein 1; Chondrocyte protein YKL40; GP 39; GP-39; GP39; HC gp39 ; HCGP 3P ; hCGP-39; HCgp39; YKL 40; YKL-40; YKL40; YYL 40

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

22-383aa

Target Protein Sequence

YKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

60.5kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Producing recombinant human chitinase-3-like protein 1 (CHI3L1) starts with isolating the target gene corresponding to the 22-383aa of human CHI3L1. This gene is co-cloned into an expression vector with an N-terminal 10xHis-SUMO-tag and C-terminal Myc-tag gene and introduced into E.coli cells via transformation. The E.coli cells express the protein, which is subsequently harvested from the cell lysate. Purification of the recombinant CHI3L1 protein is commonly achieved using affinity chromatography. The SDS-PAGE is used to assess the purity of this protein, exceeding 90%.

CHI3L1, also known as YKL-40, is a glycoprotein that belongs to the glycoside hydrolase family 18 [1]. CHI3L1 is expressed in various cell types, including colonic epithelial cells and macrophages in inflamed colons, indicating its role in inflammatory conditions [2]. It regulates inflammatory responses, gene transcriptional signaling activation, and tissue repair and remodeling responses [1][3][4]. CHI3L1 has been implicated in conditions like osteosarcoma, neurologic disorders, liver fibrosis, and cardiovascular diseases [5][6][7].

Furthermore, CHI3L1's association with inflammation and tissue damage makes it a potential biomarker in various diseases. Research indicates that fluctuations in serum CHI3L1 levels correspond with variations in liver fibrosis, highlighting its potential as a marker for tracking disease progression and therapeutic response. CHI3L1 deficiency has been linked to amelioration of liver fibrosis by promoting hepatic macrophage apoptosis [7].

References:
[1] T. Zhao, Z. Su, Y. Li, X. Zhang, & Y. Qin, Chitinase-3 like-protein-1 function and its role in diseases, Signal Transduction and Targeted Therapy, vol. 5, no. 1, 2020. https://doi.org/10.1038/s41392-020-00303-7
[2] T. Liu, J. Zhou, H. You, & J. Jia, Changes in serum chitinase 3‐like 1 levels correlate with changes in liver fibrosis measured by two established quantitative methods in chronic hepatitis b patients following antiviral therapy, Hepatology Research, vol. 48, no. 3, 2017. https://doi.org/10.1111/hepr.12982
[3] M. Kawada, Y. Hachiya, A. Arihiro, & E. Mizoguchi, Role of mammalian chitinases in inflammatory conditions, The Keio Journal of Medicine, vol. 56, no. 1, p. 21-27, 2007. https://doi.org/10.2302/kjm.56.21
[4] D. Hrabar, Ykl-40 as a biomarker in various inflammatory diseases, Biochemia Medica, vol. 34, no. 1, 2023. https://doi.org/10.11613/bm.2024.0105027
[5] M. Higashiyama, K. Tomita, N. Sugihara, H. Nakashima, H. Furuhashi, M. Nishikawaet al., Chitinase 3‐like 1 deficiency ameliorates liver fibrosis by promoting hepatic macrophage apoptosis, Hepatology Research, vol. 49, no. 11, p. 1316-1328, 2019. https://doi.org/10.1111/hepr.13396
[6] J. Yao, J. Xie, H. Wang, G. Vulugundam, H. Wang, & J. Xiao, Chitinase 3-like 1: a specifical regulator of myocardial infarction, Journal of Cardiovascular Translational Research, vol. 16, no. 3, p. 606-607, 2022. https://doi.org/10.1007/s12265-022-10344-8
[7] F. Li, A. Liu, M. Zhao, & L. Luo, Astrocytic chitinase‐3‐like protein 1 in neurological diseases: potential roles and future perspectives, Journal of Neurochemistry, vol. 165, no. 6, p. 772-790, 2023. https://doi.org/10.1111/jnc.15824

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Cell assay

Review: L6 cells were incubated with CHI3L1 (100 ng/mL) for the indicated times. Cell lysates were analyzed by Western blot using antibodies against phospho-AMPKα (Thr172) and phospho-ACC (Ser79).

By Anonymous

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■ Q&A

If I order Lyophilized form.
What should they use for solvent

Very nice to receive your inquiry. Reconstitution of lyophilized protein:
* The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on customers' experiment demand.)
* It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration.
If you have special requirements for concentration, then you could dilute the protein according to their experiment demand.
* We haven’t studied whether the optimal concentration range will vary among different hosts such as E.coli, yeast, mammalian or baculovirus, so we can’t provide useful advice
* If you will use immediately after dissolving one protein, then you don't need to add glycerol. If you won't use the protein in short term, we suggest to add some glycerol for storage.
We suggest you to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder.
You could remark "The lyophilized powder should be packed into multiple vials" when placing order. The amount of lyophilized powder in each vial will depend on your experiment demand.
* In principle, the final concentration of glycerol from 5% to 50% all will be OK. Our default final concentration of glycerol is 50%. You could use it as reference.

Target Background

Function

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

Gene References into Functions

YKL-40 elevation significantly improved risk prediction for all-cause and cardiovascular mortality in hemodialysis patients. PMID: 28941940
Polymorphisms of the CHI3L1 gene (rs4950928 C>G and rs10399931 C>T) are associated with the risk and prognosis of AD and can affect the expression of CHI3L1 in plasma. PMID: 30223258
High YKL-40 expression is associated with endothelial cell angiogenesis in breast cancer. PMID: 29274508
Pretreatment serum levels of YKL-40 may be a novel prognostic factor of overall and disease-free survival in patients with nonmetastatic colorectal cancer PMID: 29642772
Plasma YKL-40 distinguishes between patients with features of asthma-chronic obstructive pulmonary disorder (COPD) overlap (ACO) and COPD patients. PMID: 29580282
CHI3L1 expression is a novel biomarker for the prognosis of gastric cancer and these findings have thus identified CHI3L1/CD44 axis as a vital pathway and potential therapeutic target in gastric cancer PMID: 30165890
YKL-40 was highly elevated in patients with ESRD on HD, and dialysis reduced serum YKL-40 concentrations approximately one-sixth. YKL-40 measured after dialysis was independently associated with mortality in HD patients. PMID: 29357700
Review/Meta-analysis: YKL-40 may be implicated in bronchial inflammation and remodeling in COPD and may be considered as a useful biomarker for COPD diagnosis and monitoring. PMID: 29430175
YKL-40 is a glycoprotein - a potential marker for active inflammatory process - with proven predictive value in a number of diseases that evolve with inflammation, remodeling of the extracellular matrix, or development of fibrosis. PMID: 29400369
TMEM219 plays a critical role in Chi3l1-induced IL-13Ralpha2 mediated signalling and tissue responses. PMID: 27629921
Our present data reveal the crucial role of CHI3L1 in the formation of vasculogenic mimicry, which may contribute to tumour aggressiveness of cervical cancer PMID: 29452694
there was a negative correlation between YKL-40 and TLR4 expression in chronic sinusitis patients with nasal polyps. YKL-40 and TLR4 interacted with each other to activate NF-kappaB and promote disease progression. PMID: 29921378
CHI3L1 may be a previously unrecognized biomarker for diagnosing Gaucher Diseases (GD) and for evaluating the therapeutic effects of new GD drug. PMID: 29396296
Study established a connection between the pretreated CHI3L1 and patients with esophageal squamous cell carcinoma (ESCC), and the serum CHI3L1 was primarily secreted by ESCC-surrounded macrophages. PMID: 29209110
YKL-40 is expressed in cutaneous Squamous Cell Skin Cancer PMID: 30061245
The G allele of CHI3L1 rs4950928 might be a protective factor against the development of asthma. PMID: 29233108
This study suggests that CHI3L1 and CHI3L2 are associated with the progression of neurodegeneration in motor cortex and spinal cord of Amyotrophic Lateral Sclerosis patients. PMID: 28989002
in neurodegenerative dementias, YKL-40 is a disease-specific marker of neuroinflammation showing its highest levels in prion diseases PMID: 29126445
Serum IL-6, serum YKL-40, and plasma VEGF were significantly correlated with disease activity at baseline in early rheumatoid arthritis. PMID: 29336711
high serum YKL-40 levels correlated with the severity of obstructive sleep apnea syndrome and might serve as a nonspecific biomarker for prediction and progression of the disease. PMID: 29028081
YKL-40 could be considered as a useful biomarker of inflammation in psoriasis and is more sensitive than CRP or WBC. Increased YKL-40 may indicate psoriatic patients with a higher level of systemic inflammation, which may determine disease management. PMID: 28932021
YKL-40 protein expression associated with severe lung function impairment and severe exacerbations in Asthma patients. PMID: 29025889
Results suggest a potential role of chitinase 3 like 1 protein (CHI3L1) in angiogenesis in invasive ductal breast carcinoma (IDC) and may suggest its involvement in cancer progression. PMID: 29848684
YKL-40 is a promising biomarker for evaluating polymyositis/dermatomyositis-associated interstitial lung disease activity/severity and predicting disease prognosis. PMID: 28711881
Early-onset atopic dermatitis was associated with rs2303067 in SPINK5, which is involved in skin barrier functioning, and late-onset was associated with rs4950928 in CHI3L1, which is involved in the immune response. Future studies should examine the early- versus late-onset subgrouping more closely. PMID: 28681574
YKL-40 may represent a novel biomarker for predicting hypertension risk in prehypertension population PMID: 28797750
A high diagnostic sensitivity of YKL-40 in the early stages of the disease suggests the possibility of using this biomarker at an early diagnostic phase of patients with endometrial cancer. The patients with increased levels of YKL-40 before treatment are also at the higher risk of relapse. PMID: 28624692
Serum YKL-40 was elevated only in atrial fibrllation and not in other supraventricular arrhythmias. PMID: 28639986
YKL-40 is elevated in serum and bronchoalveolar lavage fluid of pulmonary alveolar proteinosis (PAP) patients, and may be of clinical utility to predict outcome in PAP PMID: 28569052
strongly expressed in placenta percreta and correlated with extravillous trophoblast invasion PMID: 27977409
YKL-40 was expressed by macrophages in liver tissue of NAFLD patients. PMID: 27739482
The results of this study indicate that YKL-40 may contribute to decreased stability and increased permeability of BBB in AD patients PMID: 28697565
YKL-40 is expressed by a subset of astrocytes in Alzheimer's disease and other tauopathies. PMID: 28599675
This study has shown that YKL-40 serum level is increased in patients with atopic dermatitis and reflects the severity of symptoms. PMID: 28660216
YKL-40 levels were found to be significantly higher in post-infectious bronchiolitis obliterans (PIBO) patients with exacerbation compared with that in bronchiolitis patients and showed a positive correlation with the severity of disease before diagnosis of PIBO. PMID: 28567534
miR-24 serves as a molecular regulator in S. aureus-induced macrophage polarization through targeting of CHI3L1 and regulation of the MAPK pathway PMID: 28303416
High YKL-40 expression is independently and markedly associated with worse overall survival in glioblastoma patients. PMID: 27090900
Datashow that patients with YKL-40 overexpression had significantly shorter survival time than those with low YKL-40 expression. PMID: 28099248
The level of serum YKL-40 is a significant and independent biomarker to predict the clinical outcome in large-artery atherosclerotic stroke. PMID: 27650381
TrkB-containing exosomes play a key role in the control of glioblastoma progression and aggressiveness, as seen in a model of YKL-40-inactivated glioblastoma cells PMID: 27385098
findings suggest that these pulmonary markers could be useful to assess CAP severity and, especially YKL-40 and CCL18 by helping predict CAP caused by atypical pathogens PMID: 29324810
The present meta-analysis indicate that elevated YKL-40 expression is associated with a poor prognosis in breast cancer patients. YKL-40 may serve as a promising predictive biomarker of prognosis of breast cancer PMID: 28036271
YKL-40 induces IL-18 production in osteoblasts and thereby stimulates angiogenesis of endothelial progenitor cells through the suppression of miR-590-3p via the focal adhesion kinase (FAK)/PI3K/Akt signaling pathway. PMID: 28448439
YKL-40 levels showed a positive correlation with blood neutrophil counts but no consistent relationships with clinical characteristics of relevance to continuous wheeze in preschool children PMID: 27732738
Study suggests that YKL-40 is associated with hypertension incidence only among men. PMID: 27815265
In HCT donors with YKL-40 plasma concentrations, a trend toward increased grade II-IV acute GvHD in recipients was observed with no significant associations with overall survival, treatment-related mortality or relapse PMID: 27427920
Review of evidence for the role of YKL-40 in the diagnosis, prognosis and cause of cardiovascular and alcoholic liver disease. PMID: 27187575
the level of CHI3L1 protein in the sera of patients with gastric or breast cancer was significantly elevated compared with those of healthy donors. M2 macrophage-secreted CHI3L1 promoted the metastasis of gastric and breast cancer cells in vitro and in vivo PMID: 28143526
Besides being associated with tau pathology, CSF YKL-40 adds to the growing array of biomarkers reflecting distinct molecular brain mechanisms potentially useful for stratifying individuals for biomarker-guided, targeted anti-inflammatory therapies emerging from precision medicine. PMID: 28281838
SNPs in CHI3L1 and cord blood YKL-40 were not associated with lung function measurements at 5 weeks and 6 years, respiratory symptoms in the first year, and allergic sensitisation at 6 years. Genetic variation in CHI3L1 might be related to the development of milder forms of asthma. PMID: 27193312

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Involvement in disease

Asthma-related traits 7 (ASRT7); Schizophrenia (SCZD)

Subcellular Location

Secreted, extracellular space. Cytoplasm. Cytoplasm, perinuclear region. Endoplasmic reticulum.

Protein Families

Glycosyl hydrolase 18 family

Tissue Specificity

Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Very low or undetectable expression in non-inflammatory colon. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

Database Links

HGNC: 1932

OMIM: 181500

KEGG: hsa:1116

STRING: 9606.ENSP00000255409

UniGene: Hs.382202

Code	CSB-EP005346HU
Abbreviation	Recombinant Human CHI3L1 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP005346HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CHI3L1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP005346HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CHI3L1.
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Recombinant Human Chitinase-3-like protein 1 (CHI3L1)

Product Details

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Target Background

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