Human Cholesteryl ester transfer protein,CETP ELISA Kit

Instructions
Code CSB-E08567h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name cholesteryl ester transfer protein, plasma
Alternative Names BPIFF ELISA Kit; CETP ELISA Kit; CETP_HUMAN ELISA Kit; Cholesteryl ester transfer protein ELISA Kit; Cholesteryl ester transfer protein plasma ELISA Kit; HDLCQ10 ELISA Kit; Lipid transfer protein I ELISA Kit
Abbreviation CETP
Uniprot No. P11597
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %89
Range %84-94
1:200Average %95
Range %90-99
1:400Average %104
Range %100-108
1:800Average %98
Range %94-102
Recovery
The recovery of human CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9594-96
EDTA plasma (n=4)10195-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.167 2.199 2.183 2.030
251.708 1.759 1.734 1.581
12.51.281 1.298 1.290 1.137
6.250.862 0.891 0.877 0.724
3.120.590 0.563 0.577 0.424
1.560.436 0.405 0.421 0.268
0.780.247 0.253 0.250 0.097
00.154 0.152 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL
Gene References into Functions
  1. Studied frequency of two genetic polymorphisms of cholesteryl ester transfer protein (CETP) in a Mongolian population with essential hypertension. PMID: 28425253
  2. The I405V polymorphism in the CETP gene is strongly associated with ischemic stroke in a Polish population. PMID: 30078763
  3. CETP may play an important role in the development of atherosclerosis mainly by decreasing HDL-C levels and increasing the accumulation of macrophage-derived foam cells PMID: 29317793
  4. Our results show an association of this CETP variant at position +82 on HDL cholesterol, levels and adiposity parameters in obese subjects with diabetes mellitus type 2 PMID: 29280647
  5. Results demonstrate that CETP expression reduced HDL and increased non-HDL fractions. Also, exercise training reduced CETP plasma levels. These results reinforce the positive metabolic effects of exercise, and resolved a controversy about CETP response to exercise PMID: 29058169
  6. APOE4 carriers and the CETP genotype were associated with a decreased response to simvastatin therapy PMID: 28851085
  7. Meta-analysis of 6 case-control studies were identified with 1494 cases and 1370 controls. An association of cholesteryl ester transfer protein (CETP) TaqIB polymorphism with ischemic stroke (IS) was found in the 4 genetic models. In the subgroup analysis by ethnicity, similar risks were also observed in Asian population. CETP TaqIB polymorphism is associated with IS risk, and the B2 allele is a protective factor. PMID: 28648960
  8. The aggregation analysis revealed a higher correlation between siblings than between parent-offspring pairs representing the role of genetic factors in metabolic syndrome (MetS). In addition, the conditional logistic model with covariates showed that the linkage results between HDL_C and three markers, FTO (rs1558902 and rs7202116) and CETP(rs1864163) were significant. PMID: 29548861
  9. Regression analysis revealed significant risk for memory loss that are dependent on age and genetic variants like CETP. PMID: 28777751
  10. The -629C allele was significantly associated with an increased risk of Coronary heart disease in Caucasians, and this association may be mediated by its phenotypic regulation on circulating CETP and HDL-C. PMID: 27791990
  11. inhibitors, especially Torcetrapib and Anacetrapib, increased the binding ratios of the binary complexes (e.g., HDL-CETP and LDL-CETP) and decreased the binding ratios of the HDL-CETP-LDL ternary complexes. PMID: 28911944
  12. Studied association of Cholesterol Ester Transfer Protein (CETP) genetic polymorphisms with acute coronary syndrome; found the frequency of certain genotypes was significantly lower in unstable angina patients, compared with the control group PMID: 28387842
  13. Inhibiting cholesteryl ester transfer protein activity inhibits vascular smooth muscle cell proliferation, and reduces neointimal hyperplasia in an scavenger receptor-B1, PDZ domain-containing protein 1- and phosphatidylinositol-3-kinase/Akt-dependent manner. PMID: 29025709
  14. The rs1800775, rs3764261, rs12149545, rs711752, and rs708272 polymorphisms of CETP were associated with metabolic syndrome and its components among the Uyghur ethnic group. Complete linkage disequilibrium was found between two pairs of SNPs (rs3764261 and rs12149545, rs711752, and rs708272). PMID: 28629169
  15. CETP-inhibition, although effective in improving the lipid profile, is not associated with vascular protective effects as assessed by endothelial function. PMID: 28152406
  16. The study identified two polymorphisms with a higher risk of age-related macular degeneration development (AMD) and also a protective polymorphism for AMD. PMID: 28918250
  17. CETP TaqIB polymorphism protects against composite ischemic CVD risk and is associated with a higher HDL-C concentration in both Asians and Caucasians (Meta-Analysis) PMID: 27608031
  18. Combined exposure to variants in the genes that encode the targets of CETP inhibitors and statins was associated with discordant reductions in LDL-C and apoB levels and a corresponding risk of cardiovascular events that was proportional to the attenuated reduction in apoB but significantly less than expected per unit change in LDL-C. PMID: 28846118
  19. Treatment of mildly hypercholesterolemic subjects with CETP inhibitor anacetrapib reduces Lp(a) levels by decreasing its production. PMID: 28729361
  20. Carriers of protein-truncating variants of CETP displayed higher high-density lipoprotein cholesterol and lower risk for coronary heart disease. PMID: 28506971
  21. Based on results from the study with a relatively large sample size, CETP may be the only detectable locus with common genetic variants that influence HDL-C response to statins substantially in individuals of European descent. Although CETP is known to be associated with HDL-C, the evidence is provided that this pharmacogenetic effect is independent of its association with baseline HDL-C levels. PMID: 27587472
  22. The rs1800775 [1.31 (1.22-1.42); p = 3.41E-12] in the CETP gene and rs359027 [1.26 (1.16-1.36); p = 2.55E-08] in the LMCD1 gene were significantly associated with LHDLC levels PMID: 26879886
  23. CETP modulates the distribution of spingosine-1-phosphate among lipoproteins, which affects the bioactivities of S1P. PMID: 28126827
  24. Anacetrapib treatment increases HDL apoA-I and CETP levels by decreasing the fractional clearance rate of each protein. PMID: 26966279
  25. Since expression of CETP has been shown to be positively correlated with the risk of CAD, higher frequency of "A" allele (patients: 22.69% vs.controls: 13%) reveals that c.*84G>A is a risk factor for CAD in South Indians PMID: 27768712
  26. CETP V405V genotype was associated with significantly enhanced HDL levels (P = 0.03), mostly owing to the female sex (P = 0.46 for males, P = 0.02 for females), whereas LDL and triglyceride levels were unchanged. PMID: 27439317
  27. The single nucleotide polymorphisms in lipoprotein lipase, ApoA5, and CETP were associated with serum triglycerides and HDL-cholesterol levels, but not with coronary artery disease in Pakistani population under study. PMID: 28143480
  28. Elevated CETP blood concentration was risk factor for coronary artery disease and correlates with pre beta1-high-density lipoprotein. PMID: 28073362
  29. Results show that the bound cholesteryl esters intraconvert between bent and linear conformations in the CETP core tunnel as a consequence of the high degree of conformational flexibility of the protein. PMID: 27445332
  30. Daily consumption of policosanol for 8 weeks resulted in lowered blood pressure, reduced serum TG level and CETP activity, and elevated HDL-C contents. PMID: 28259941
  31. serum CETP and PLTP activity in patients diagnosed with hypothyroidism, was examined. PMID: 27899788
  32. Elevated CETP Lipid Transfer Activity is Associated with the Risk of Venous Thromboembolism. PMID: 27169917
  33. it can be concluded that the effects of the CETP variation on LDL subfraction could change in cardiometabolic events such as coronary heart disease and statin therapy. PMID: 27900488
  34. Genetic variants in CETP associated with increased plasma high-density lipoprotein cholesterol raise the risk of intracerebral hemorrhage PMID: 27717122
  35. CETP Taq1B Polymorphisms are associated with Hyperlipidemia. PMID: 27590083
  36. The study has identified a novel ethnic-specific gene-gene interaction and suggested that the combination of cholesteryl ester transfer protein B1 allele and endothelial nitric oxide synthase 4a allele significantly increases the risk of coronary artery disease in Malays and Indians. PMID: 25667236
  37. Both PCSK9 levels and CETP activity were higher in patients with an increasing number of metabolic syndrome components PMID: 27488210
  38. Our results suggest that there is no significant association between CETP I405V polymorphism and the risk of coronary artery disease presence and severity PMID: 26773179
  39. Data show that polymorphisms of rs662799 and rs2266788 in APOA5 gene, rs320 in LPL gene and rs708272 in CETP gene had significant association with the effect of the lipid-lowering therapy via atorvastatin on ischemic stroke patients. PMID: 27415775
  40. Study provides no evidence for CETP gene rs708272 polymorphism association with metabolic syndrome despite its influence on its blood protein concentration. PMID: 27496123
  41. results showed that a hydrophobic tunnel inside CETP is sufficient to allow a CE molecule to completely transfer through the entire CETP within a predicted transfer time and at a rate comparable with those obtained through physiological measurements PMID: 27143480
  42. SNPs at the CETP, HNF4A and KLF14 locus are associated with HDL-C levels and type 2 diabetes (in female participants). PMID: 26670163
  43. Cholesteryl ester transfer between HDL and LDL lipoproteins does not require a ternary tunnel complex with CETP. PMID: 26876146
  44. No evidence for clinically relevant associations between several measures of body fat and serum CETP concentration. This finding implies that adipose tissue does not contribute to the CETP pool in serum. PMID: 26820801
  45. This is the first report on the association of these polymorphisms with Coronary artery disease (CAD) in Saudi Arabia. The rs5882 polymorphism (CETP) showed a significant association and therefore could be a promising marker for CAD risk PMID: 26936456
  46. A single-nucleotide polymorphism in the cholesteryl ester transfer protein (CETP) gene (isoleucine to valine; V405) is associated with slower memory decline and a lower risk of Alzheimer's disease. PMID: 27033407
  47. CETP per se has no impact on the glucose tolerance and tissue uptake, global insulin sensitivity and beta cell insulin secretion rates. PMID: 26758205
  48. Study suggests that the II genotype of the CETP I405V polymorphism is associated with significant abnormalities in gray matter microstructure compared to those with a V405 allele PMID: 26253899
  49. The cholesteryl ester transfer protein (CETP) rs3764261 variant was significantly associated with an increased risk of AMD PMID: 26503844
  50. The polymorphism of CETP genes rs708272, rs3764261, rs1800775, rs711752, rs12149545 was closely related to the dyslipidemia in the Xinjiang Uyghur and Kazakh ethnic groups; and the rs708272 T, rs3764261 T, rs711752 A, and rs12149545 A alleles could reduce risk of dyslipidemia in the Uyghur and Kazakh populations, however, the rs1800775 C allele showed risk factors PMID: 26694435
  51. Presence of CETP SNP rs3764261 is not associated with insulin resistance and incident T2DM in patients with clinically manifest vascular disease PMID: 26318399
  52. Our data suggested that the allele A in rs3764261 in CETP gene may be associated with a decreased risk of advanced AMD in Chinese population. PMID: 24498989
  53. polymorphisms in CETP protect HIV-infected patients from developing atherogenic dyslipidemia in a dose-dependent manner PMID: 26101956
  54. Cholesteryl ester transfer protein facilitates the exchange of triglycerides from TG-rich VLDL-1 particles for cholesteryl esters from high-density lipoprotein (HDL) particles. PMID: 26244602
  55. our results show the involvement of the variants of ESR1, LPL and CETP genes in metabolic events related to MetS or some of its features. PMID: 26370976
  56. decreased triglyceride content of CETP-deficient cells arises from the reduced conversion of DG to TG in the ER and/or on the lipid droplet surface, and enhanced TG degradation in the ER due to its ineffective transport from this organelle. PMID: 26203075
  57. CETP can protect against the obesity-induced impairment in exercise capacity and may be a target to improve exercise capacity in the context of obesity PMID: 26313355
  58. SNPs in SORT1, CETP and GCKR were individually associated with lipid level variations in Algerian population. PMID: 26261636
  59. results point out that in addition to the common CETP variants, rare genetic variants in the CETP gene also contribute to the phenotypic variation of HDL-C in the general population PMID: 26683795
  60. No significant difference was found in the CETP gene I405V and D442G genotypes between the cerebral hemorrhage patients and controls. PMID: 26829743
  61. Although opposing effects suggest that regulation of CETP expression could vary between tissues, the minor allele of rs247616 and single nucleotide polymorphisms in high linkage with it were found to be associated with reduced expression. PMID: 26061659
  62. Data indicate that the high-density lipoproteins (HDL) surface lipid curvature generates a hydrophobic environment, leading to cholesteryl ester transfer protein (CETP) hydrophobic distal end interaction. PMID: 25737239
  63. Gene-environment interactions studies found significance association between selenium and rs2290201 in FABP4 for total and LDL cholesterol levels and rs1800774 in CETP for elevated LDL cholesterol. PMID: 25606668
  64. CETP TaqIB and ApoE polymorphisms may not be considered as genetic risk factors for MetS in a Southern Thai population. epsilon4 allele, associated with one metabolic component, low HDL-C levels, might predispose the subjects to develop metabolic disturbances. PMID: 26014924
  65. circulating CETP and Lp-PLA2 might partly play a role in the atherogenic disturbances in patients with iron deficiency anemia through increased susceptibility to lipid peroxidation PMID: 26642708
  66. findings demonstrate that the long-term genetically reduced circulating CETP might be causally associated with the low risk of CHD. PMID: 25561046
  67. Identify novel associations between elevated CETP activity and quantitative/qualitative lipoprotein abnormalities in the atherogenic dyslipidemia in South Asians, a major contributor of increased atherosclerotic cardiovascular diseases in South Asians. PMID: 24659026
  68. B2 allele is associated with higher concentrations of HDL-C and lower concentrations of CETP, which confer a protective effect on coronary artery disease. PMID: 25725138
  69. These data show that overexpression of full-length CETP disrupts lipid homeostasis resulting in the formation of smaller, more metabolically active lipid droplets. PMID: 25593327
  70. study provides replicable evidence that individuals with the CETP rs3764261 CC genotype might derive greater effects on raising HDL cholesterol and lowering triglycerides by choosing a low-carbohydrate/high-fat weight-loss diet instead of a low-fat diet. PMID: 25548261
  71. genetic polymorphism is associated with coronary artery disease in Egyptians PMID: 25110219
  72. Its single nucleotide polymorphism implicated in brain diseases including Alzheimer's disease. PMID: 24997672
  73. Data from Brazilian family with familial apoA-I deficiency with nonsense mutation at codon -2 of APOA1 (apolipoprotein A-I) suggest plasma CETP activity is significantly increased in both homozygotes and heterozygotes versus control subjects. PMID: 25341944
  74. The aim of this study was to investigate the influence of genetic polymorphism of CETP gene polymorphism -629C/A on the therapeutic effect of atorvastatin and clinical outcome in Han Chinese patients with coronary heart disease. PMID: 25547030
  75. CETP I405V (rs5882) is associated with cognitive function and late-onset Alzheimer's disease risk. PMID: 25260850
  76. novel atomistic details of the CETP-HDL interaction mechanism PMID: 25424006
  77. increased CETP activity during acute phase of STEMI is independently associated with endothelial dysfunction and adverse clinical outcome PMID: 25463120
  78. The presence of the B1B1 polymorphism of the Taq1B CETP genotype contributes to the development of atrial fibrillation in the Han and Kazak populations in western China PMID: 24615107
  79. TaqIB (rs708272) polymorphism modifies association between alcohol and coronary heart disease PMID: 25288221
  80. rs1532624 Single Nucleotide Polymorphism of CETP gene is associated with reduced response to statin therapy. PMID: 24160573
  81. We demonstrate a significant association of LPL and CETP polymorphisms with serum triglycerides and HDL-cholesterol. PMID: 25579610
  82. There are no differences in the enzymatic activity of CETP among the carriers of different genotypes. PMID: 25696989
  83. The CETP gene TaqIB polymorphism B2B2 genotype was associated with higher levels of serum HDL cholesterol and a more favorable lipid profile. PMID: 25073458
  84. CETP polymorphisms may influence plasma lipid levels in Greek men with coronary heart having BMI variations PMID: 24402318
  85. The overall comparison of the B2 allele with the B1 allele of cholesteryl ester transfer protein yielded a significant risk reduction of coronary artery disease with substantial between-study heterogeneity. PMID: 25366166
  86. cholesterol may competitively inhibit cholesteryl ester binding to the CETP molecule, with triglyceride binding being largely undisturbed. PMID: 25201589
  87. The phospholipid matrix in discoidal HDL may underlie CETP activity through the self-association, diffusivity and location of cholesteryl ester in the bilayer PMID: 25449063
  88. Results suggest the B2B2 genotype of the cholesteryl ester transfer protein TaqIB polymorphism is a protective factor against the development of myocardial infarction. PMID: 25474428
  89. CETP 405V is a risk allele against longevity in Chinese. PMID: 23977315
  90. The ability of apoC1 to inhibit CETP activity is impaired in patients with diabetes. Glycation of apoC1 leads to a change in its electrostatic properties that might account, at least in part, for a loss of constitutive CETP inhibition and an increase in plasma CETP activity in patients with diabetes. PMID: 24574346
  91. Gene-gene interaction between CETP and APOE polymorphisms confers higher risk for hypertriglyceridemia in oldest-old Chinese women. PMID: 24746514
  92. CETP rs708272 (C>T) and rs1800775 (C>A) polymorphisms may contribute to myocardial infarction susceptibility [meta-analysis] PMID: 24533069
  93. Data (including data from studies in transgenic mice) suggest expression of CETP in liver can be regulated by dietary factors; here, supplementation with fish oil up-regulates expression of CETP in liver and has hypolipidemic affects. PMID: 24746832
  94. Results conclude that the R451Q polymorphisms in the CETP gene had no effects on blood lipid levels and are not a risk factor for CHD in Han and Uyghur Chinese. PMID: 24634116
  95. A novel mechanism of lipid transfer carried out by CETP. PMID: 24530617
  96. A meta-analysis found that individuals with the CETP rs5882 A>G polymorphism were found to have a higher risk of developing Alzheimer's disease. PMID: 25105518
  97. Polymorphisms of the cholesteryl ester transfer protein gene is associated with abdominal obesity. PMID: 25115339
  98. There is an association between its polymorphism and the risk of Alzheimer's disease in the Asian populations with APOE+. PMID: 24468472
  99. the CETP TaqIB polymorphism is associated with the lipid-lowering effect of atorvastatin in patients with CHD. PMID: 24737438
  100. Variants with CETP conferred susceptibility to CAD in overweight individuals. PMID: 25134189

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Involvement in disease Hyperalphalipoproteinemia 1 (HALP1)
Subcellular Location Secreted, extracellular space
Protein Families BPI/LBP/Plunc superfamily, BPI/LBP family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

HGNC: 1869

OMIM: 118470

KEGG: hsa:1071

STRING: 9606.ENSP00000200676

UniGene: Hs.89538

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