Human Cholesteryl ester transfer protein,CETP ELISA Kit

Code CSB-E08567h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P11597
Lead Time 3-5 working days
Protein Biological Process 2 Lipogenesis and lipometabolism
Abbreviation CETP
Protein Biological Process 1 Biosynthesis/Metabolism
Target Name cholesteryl ester transfer protein, plasma
Alias HDLCQ10, lipid transfer protein
Species Homo sapiens (Human)
Protein Biological Process 3 Cholesterol metabolism
Sample Types serum, plasma, tissue homogenates
Detection Range 0.78 ng/mL-50 ng/mL
Sensitivity 0.195 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Cholesteryl ester transfer protein (CETP) transfers cholesteryl esters between lipoproteins. CETP may effect susceptibility to atherosclerosis.
HGNC 1869
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %89
Range %84-94
1:200Average %95
Range %90-99
1:400Average %104
Range %100-108
1:800Average %98
Range %94-102
Recovery
The recovery of human CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9594-96
EDTA plasma (n=4)10195-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.167 2.199 2.183 2.030
251.708 1.759 1.734 1.581
12.51.281 1.298 1.290 1.137
6.250.862 0.891 0.877 0.724
3.120.590 0.563 0.577 0.424
1.560.436 0.405 0.421 0.268
0.780.247 0.253 0.250 0.097
00.154 0.152 0.153  
References
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Function Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL
Involvement in disease Hyperalphalipoproteinemia 1 (HALP1)
Subcellular Location Secreted, extracellular space
Protein Families BPI/LBP/Plunc superfamily, BPI/LBP family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

HGNC: 1869

OMIM: 118470

KEGG: hsa:1071

STRING: 9606.ENSP00000200676

UniGene: Hs.89538

Pathway Cholesterol metabolism

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