Human Cocaine-and amphetamine-regulated transcript protein (Cartpt) ELISA kit

Instructions
Code CSB-EL004532HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Cocaine-and amphetamine-regulated transcript protein (Cartpt)
Alternative Names CART ELISA Kit; CART prepropeptide ELISA Kit; CART(42-89) ELISA Kit; CART_HUMAN ELISA Kit; Cartpt ELISA Kit; Cocaine and amphetamine regulated transcript ELISA Kit
Abbreviation Cartpt
Uniprot No. Q16568
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Cartpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 91
Range % 84-99
1:2 Average % 88
Range % 81-97
1:4 Average % 95
Range % 92-100
1:8 Average % 94
Range % 86-98
Recovery
The recovery of human Cartpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 94 90-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.598 2.627 2.613 2.508
500 2.248 2.296 2.272 2.167
250 1.811 1.795 1.803 1.698
125 1.186 1.201 1.194 1.089
62.5 0.643 0.639 0.641 0.536
31.2 0.368 0.359 0.364 0.259
15.6 0.194 0.202 0.198 0.093
0 0.103 0.106 0.105  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro.
Gene References into Functions
  1. Data suggest that the stress of intermittent hypoxia up-regulates expression of mRNA and protein for POMC and CART in neuronal cell lines; GATA2 and GATA3 appear to be involved in these stress mechanisms. (POMC = proopiomelanocortin; CART = cocaine- and amphetamine-regulated transcript protein; GATA2 = endothelial transcription factor GATA-2; GATA3 = T-cell-specific transcription factor GATA-3) PMID: 29275211
  2. Adenocarcinoma growth into the colon wall caused an increase in the relative frequency of CART-like immunoreactive (CART-LI) neurons in enteric plexuses located in the vicinity of the infiltrating neoplasm. PMID: 29385033
  3. In vitro neurochemical studies provide new insights into the interactions among CART peptide, dopamine and cocaine in the nucleus accumbens by showing a direct inhibitory effect of CART on dopamine both in normal conditions and after cocaine induction, as well as an effect of CART on the dopamine metabolites DOPAC, HVA and DOPET. PMID: 28802898
  4. Study conclude that CART is a regulator of glucose homeostasis and could play an important role in the pathophysiology of type 2 diabetes. Based on the ability of CART to increase insulin secretion and reduce glucagon secretion, CART-based agents could be a therapeutic modality in type 2 diabetes. PMID: 27338624
  5. polymorphisms in the gene may be useful in identifying women at risk for osteoporosis. PMID: 25871004
  6. In samples from patients undergoing IVF, there is a significant positive correlation between patient BMI, CART mRNA expression in granulosa cells, and CART peptide levels in follicular fluid. PMID: 26730935
  7. Colocalization experiments provided evidence for the presence of cocaine- and amphetamine-regulated transcript (CART) in KP-immunoreactive (IR) and perikarya and in KP-IR and NKB-IR axon varicosities. PMID: 25084101
  8. In schizophrenia patients on clozapine monotherapy, CART levels did not correlate with age, weight, BMI, abdominal, waist and hip circumferences, WHR, blood pressure, clozapine dose or treatment duration, body composition, or markers of insulin resistance PMID: 25219614
  9. Lower CART levels pose a risk for developing polycystic ovary syndrome. PMID: 24995854
  10. CART SNPs (rs10515115, rs3763153, rs3857384, rs11575893, rs16871471) were not associated with antipsychotic-induced weight gain in schizophrenia patients. PMID: 24564533
  11. CART is expressed in nerve fibers and co-localizes with other neuronal factors in the circular muscle layer of human descending colon PMID: 23277430
  12. Family-based association study failed to observe a statistically significant association for any of the genotyped SNPs in the CARTPT gene and attention-deficit hyperactivity disorder. PMID: 22436349
  13. Ischemia insult triggered by oxygen-glucose deprivation (OGD) enhances NRSF mRNA levels and then NRSF antagonizes the CREB signaling on CART activation, leading to augmented cell death. PMID: 23086924
  14. Serum CART can be detected in both diabetic and non-diabetic human pregnancy and may play an important role in body mass regulation in pregnancy. PMID: 22332848
  15. a detailed description of the CART distribution pattern within the human alimentary tract. PMID: 23034261
  16. The first report on the presence of CART-IR structures in the normal human pancreas. PMID: 22257587
  17. Expression of CART in small bowel carcinoid tumors is associated with worse survival. PMID: 22553341
  18. It was shown that cocaine- and amphetamine-regulated transcript protein (CART) increases the transcriptional activity of estrogen receptor alpha and that CART stimulates an autocrine/paracrine loop within tumor cells to amplify the CART signal. PMID: 22139072
  19. This study demonistrated thatsex difference in CART dynamic expressions in the midbrain of drug-free depressed suicide victims. PMID: 21803054
  20. No significant correlations are found between cerebrospinal fluid levels of orexin A and CART, fatigue, and hypersomnolence in multiple sclerosis patients. PMID: 21605873
  21. CART is an antioxidant peptide PMID: 22235287
  22. The CART c.517A-->G polymorphism was significantly associated with obesity susceptibility. PMID: 21921635
  23. CART is produced in the majority of neuroendocrine tumors, regardless of tumor origin. PMID: 21832817
  24. genetic variants in the CART gene may not play a major role in the vulnerability of schizophrenia subjects to concurrent alcohol use disorders and nicotine dependence. PMID: 20362026
  25. Our results supported the hypothesis that inherited variations of the CART gene could influence the development of obesity also in Italian children. PMID: 20151954
  26. genetic variants involved in weight gain, development of obesity PMID: 11791158
  27. Review. The genetics of CART and its role in reward, stress, and feeding behavior are reviewed. PMID: 12147208
  28. AvaII polymorphism (rs2239670) in intron 1 of the CART gene is associated with alcoholism in the Korean male population. PMID: 15234472
  29. Mutating codon 34 of human proCART alters the levels of CART peptides in transfected AtT20 cells. PMID: 15326462
  30. CART SNP -3608T>C may possibly contribute to the genetic risk for obesity in the Caucasian population PMID: 15823203
  31. CART has a role in the control of appetite [review] PMID: 16102267
  32. Cocaine- and amphetamine-regulated transcript peptides (55 to 102 & 62 to 102) are neurotransmitters with roles in a number of processes, including drug abuse by virtue of the fact that they are modulators of mesolimbic function [Review] PMID: 16146347
  33. Obesity observed in humans bearing the Leu34Phe mutation could be due to a putative deficiency in hypothalamic bioactive CART. PMID: 16210370
  34. analysis of CART binding sites on HepG2 cells and mouse hypothalamic dissociated cells PMID: 16309793
  35. CART signaling is the main molecular pathway accounting for the decrease in bone resorption leading to high bone mass in mice and humans deficient in Mc4r PMID: 16614075
  36. The anatomy of CART-containing neurons in the human infundibular nucleus raises the question as to whether this is asssociated with neuropeptide Y and agouti related protein. PMID: 17525122
  37. reviews the localization of CART mRNA expression in the human brain and its possible relevance to human cocaine abuse [review] PMID: 17631364
  38. This study do not support an association of the CARTPT gene with cocaine dependence. PMID: 18572320
  39. Data show that CSF CART levels are significantly reduced by 30% in Dementia with Lewy bodies patients compared to controls as well as to Alzheimer's disease patients using radioimmunoassay. PMID: 19356602
  40. Medium-sized CART-positive neurons are common in the medial division of the shell of nucleus accumbens in monkeys. CART may be involved in neuronal and behavioural changes that underlie addiction to psychomotor stimulants and feeding. PMID: 10235641
  41. The solution structure of the biologically active part of CART (residues 48-89) consists of turns and loops spanned by a compact framework composed of a few small stretches of antiparallel beta-sheet common to cystine knots. PMID: 11478874

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Subcellular Location Secreted
Protein Families CART family
Tissue Specificity Hypothalamus. Found in neurons of the ventrolateral part of the arcuate nucleus, in the external zone of the median eminence, and also found in terminals in the periventricular part of the paraventricular nucleus.
Database Links

HGNC: 24323

OMIM: 602606

KEGG: hsa:9607

STRING: 9606.ENSP00000296777

UniGene: Hs.1707

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