Human Coenzyme Q10 (CoQ10) ELISA Kit

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Human Coenzyme Q10 (CoQ10) ELISA Kit

Name: Human Coenzyme Q10 (CoQ10) ELISA Kit
Brand: CUSABIO
SKU: CSB-E14081h

^Instructions

Code	CSB-E14081h
Size	96T,5×96T,10×96T
Trial Size
Have Questions?	Leave a Message or Start an on-line Chat

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Target Name

Coenzyme Q10 (CoQ10)

Alternative Names

N/A

Abbreviation

CoQ10

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12 ng/mL-50 ng/mL

Sensitivity

1.56 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	96
	Range %	91-101
1:40	Average %	99
	Range %	93-104
1:80	Average %	92
	Range %	87-96
1:160	Average %	90
	Range %	85-94

Recovery

The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	93	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
50	0.112	0.103	0.108
25	0.208	0.224	0.216
12.5	0.377	0.397	0.387
6.25	0.783	0.718	0.751
3.12	1.121	1.086	1.104
0	1.951	1.829	1.890

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Ubiquinone Levels as a Marker of Antioxidant System in Children with Attention Deficit Hyperactivity Disorder YS Karagöz,Journal of molecular neuroscience,2020
An Open Pilot Study of the Effect and Tolerability of Add-On Multivitamin Therapy in Patients with Intractable Focal Epilepsy HH Chang,Nutrients,2020
Serum Coenzyme Q10 Levels are Decreased in Critically-Ill Septic Patients: Results From a Preliminary Study AG Vassiliou,Biol Res Nurs,2020
Clinical phenotype, in silico and biomedical analyses, and intervention for an East Asian population-specific c.370G>A (p.G124S) COQ4 mutation in a Chinese family with CoQ10 deficiency-associated Leigh syndrome Lu M, et al,Journal of Human Genetics,2019
The effect of Ubiquinone administration on oxidative DNA damage and repair in plasma levels in non-proliferative diabetic retinopathy Sonia Sifuentes-Franco1.et al,Diabetes Management,2017
The Effect of Coenzyme Q10 Administration on DNA Damage in Diabetic Polyneuropathy Sandra Carrillo-Ibarra.et al,Journal of Global Diabetes & Clinical Metabolism,2017

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■ Q&A

Please provide the following information for Human Coenzyme Q10, CoQ10 ELISA Kit (cat:CSB-E14081h):
1.What is the standard?
2.What is the antibody in this kit?
3. Is the antibody against lipid Coq10 or to its binding protein?
4. will binding protein be labeled to Co1q10 or directly coq10?

Thanks for your inquiry.
The standard is purified CoQ10.
This kit detects total CoQ10, and the antibody in the kit is mouse monoclonal antibody.
The antibody is specific to CoQ10.Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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