Human Coenzyme Q10 (CoQ10) ELISA Kit

Code CSB-E14081h
Size 96T,5×96T,10×96T
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Product Details

Target Name
Coenzyme Q10 (CoQ10)
Alternative Names
N/A
Abbreviation
CoQ10
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/mL-50 ng/mL
Sensitivity
1.56 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 96  
Range % 91-101  
1:40 Average % 99  
Range % 93-104  
1:80 Average % 92  
Range % 87-96  
1:160 Average % 90  
Range % 85-94  
Recovery
The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 80-88  
EDTA plasma (n=4) 93 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
50 0.112 0.103 0.108    
25 0.208 0.224 0.216    
12.5 0.377 0.397 0.387    
6.25 0.783 0.718 0.751    
3.12 1.121 1.086 1.104    
0 1.951 1.829 1.890    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Coenzyme Q10 is a lipophilic quinone that plays an important role in cellular energy production within the mitochondrial electron transport chain. As a key component of oxidative phosphorylation, CoQ10 supports ATP synthesis and serves as a potent antioxidant, protecting cellular membranes from oxidative damage. This ubiquinone derivative is naturally synthesized in human tissues. Concentrations decline with age and in various pathological conditions, making it an important biomarker for metabolic health and mitochondrial function research.

The Human Coenzyme Q10 ELISA Kit uses a competitive quantitative assay principle to measure CoQ10 levels in serum, plasma, and tissue homogenates. The kit offers a detection range of 3.12 ng/mL to 50 ng/mL with a sensitivity of 1.56 ng/mL, requiring 50-100 μL sample volumes for analysis. Detection occurs at 450 nm wavelength. Assay completion takes 1-5 hours, providing researchers with a reliable tool for CoQ10 quantification in human samples.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in research focused on mitochondrial function and vitamin supplementation studies. The kit allows quantification of CoQ10 levels in cellular and serum samples to evaluate mitochondrial health and monitor nutritional status.

Mitochondrial function research: Measurement of CoQ10 concentrations in cell culture systems to evaluate mitochondrial health and cellular bioenergetics
Nutritional supplementation studies: Monitoring of serum CoQ10 levels in clinical trials examining the effects of multivitamin supplementation over extended treatment periods
Cellular metabolism: Analysis of CoQ10 content in homogenized cell samples following various experimental treatments to understand metabolic responses

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Please provide the following information for Human Coenzyme Q10, CoQ10 ELISA Kit (cat:CSB-E14081h):
1.What is the standard?
2.What is the antibody in this kit?
3. Is the antibody against lipid Coq10 or to its binding protein?
4. will binding protein be labeled to Co1q10 or directly coq10?

A:
Thanks for your inquiry.
The standard is purified CoQ10.
This kit detects total CoQ10, and the antibody in the kit is mouse monoclonal antibody.
The antibody is specific to CoQ10.Pls let me know if you have any further questions. Thank you.
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