Human Coenzyme Q10 (CoQ10) ELISA Kit

Instructions
Code CSB-E14081h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Coenzyme Q10 (CoQ10)
Alternative Names N/A
Abbreviation CoQ10
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-50 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 96  
Range % 91-101  
1:40 Average % 99  
Range % 93-104  
1:80 Average % 92  
Range % 87-96  
1:160 Average % 90  
Range % 85-94  
Recovery
The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 80-88  
EDTA plasma (n=4) 93 88-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
50 0.112 0.103 0.108    
25 0.208 0.224 0.216    
12.5 0.377 0.397 0.387    
6.25 0.783 0.718 0.751    
3.12 1.121 1.086 1.104    
0 1.951 1.829 1.890    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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 Q&A
Q:

Please provide the following information for Human Coenzyme Q10, CoQ10 ELISA Kit (cat:CSB-E14081h):
1.What is the standard?
2.What is the antibody in this kit?
3. Is the antibody against lipid Coq10 or to its binding protein?
4. will binding protein be labeled to Co1q10 or directly coq10?

A:
Thanks for your inquiry.
The standard is purified CoQ10.
This kit detects total CoQ10, and the antibody in the kit is mouse monoclonal antibody.
The antibody is specific to CoQ10.Pls let me know if you have any further questions. Thank you.

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