Human Coll2-1 ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Citations (4) Protocol MSDS

Code	CSB-EQ027311HU
Size	96T,5×96T,10×96T
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Product Details
Citations (4)
Customer Reviews and Q&A

Abbreviation

Coll2-1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, cell culture supernates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Others

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	85
	Range %	80-90
1:200	Average %	98
	Range %	90-105
1:400	Average %	95
	Range %	90-100
1:800	Average %	92
	Range %	87-97

Recovery

The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	83-95
EDTA plasma (n=4)	98	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.513	2.487	2.500	2.417
1500	1.847	1.794	1.821	1.738
750	1.198	1.112	1.155	1.072
375	0.754	0.709	0.732	0.649
187.5	0.351	0.337	0.344	0.261
94	0.198	0.183	0.191	0.108
47	0.134	0.125	0.130	0.047
0	0.084	0.081	0.083

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human Coll2-1 ELISA Kit was designed for the quantitative measurement of Human Coll2-1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.

Cartilage Regeneration Potential of Platelet-Rich Plasma versus Plasma Rich in Growth Factors in Knee Osteoarthritis Based on Serum Biomarker of Cartilage Degradation, Coll2-1: A Triple-Blind Randomized Study A Saraf, H Habib, S Bishnoi,Journal of Orthopaedic Case Reports,2025
Osteochondral Tissue-On-a-Chip: A Novel Model for Osteoarthritis Research I González-Guede, D Garriguez-Perez,International Journal of Molecular Sciences,2024
Serial intraarticular injections of growth factor concentrate in knee osteoarthritis: A placebo controlled randomized study A Saraf,Journal of Orthopaedics,2023
Cartilage oligomeric protein, matrix metalloproteinase-3, and Coll2-1 as serum biomarkers in knee osteoarthritis: a cross-sectional study Georgiev T.et al,Rheumatol Int,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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