Human Coll2-1 ELISA kit

Code CSB-EQ027311HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Abbreviation
Coll2-1
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates
Detection Range
47 pg/mL-3000 pg/mL
Sensitivity
11.75 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Coll2-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 85  
Range % 80-90  
1:200 Average % 98  
Range % 90-105  
1:400 Average % 95  
Range % 90-100  
1:800 Average % 92  
Range % 87-97  
Recovery
The recovery of human Coll2-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 83-95  
EDTA plasma (n=4) 98 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.513 2.487 2.500 2.417  
1500 1.847 1.794 1.821 1.738  
750 1.198 1.112 1.155 1.072  
375 0.754 0.709 0.732 0.649  
187.5 0.351 0.337 0.344 0.261  
94 0.198 0.183 0.191 0.108  
47 0.134 0.125 0.130 0.047  
0 0.084 0.081 0.083    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human Coll2-1 ELISA Kit was designed for the quantitative measurement of Human Coll2-1 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.

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