Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

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Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Name: Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT
Brand: CUSABIO
SKU: CSB-E04799h

^Instructions

Code	CSB-E04799h
Size	96T,5×96T,10×96T
Trial Size
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Target Name

Collagen Type Ⅲ,Col Ⅲ

Alternative Names

Collagen Type 3,Col 3,Collagen Type III,Col III

Abbreviation

Col Ⅲ

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

12.5 ng/mL-800 ng/mL

Sensitivity

3.12 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	102
	Range %	97-105
1:4	Average %	92
	Range %	85-98
1:8	Average %	97
	Range %	91-102

Recovery

The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-94
EDTA plasma (n=4)	97	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
800	2.850	2.963	2.907	2.778
400	2.255	2.203	2.229	2.100
200	1.279	1.256	1.268	1.139
100	0.659	0.655	0.657	0.528
50	0.438	0.475	0.457	0.328
25	0.256	0.242	0.249	0.120
12.5	0.184	0.172	0.178	0.049
0	0.126	0.132	0.129

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.