Target Name |
Collagen Type Ⅲ,Col Ⅲ |
Alternative Names |
Collagen Type 3,Col 3,Collagen Type III,Col III |
Abbreviation |
Col Ⅲ |
Species |
Homo sapiens (Human) |
Sample Types |
serum, plasma, tissue homogenates |
Detection Range |
12.5 ng/mL-800 ng/mL |
Sensitivity |
3.12 ng/mL |
Assay Time |
1-5h |
Sample Volume |
50-100ul |
Detection Wavelength |
450 nm |
Research Area |
Signal Transduction
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Assay Principle |
quantitative |
Measurement |
Sandwich |
Precision |
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity |
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
Sample |
Serum(n=4) |
|
1:1 |
Average % |
92 |
|
Range % |
86-96 |
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1:2 |
Average % |
102 |
|
Range % |
97-105 |
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1:4 |
Average % |
92 |
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Range % |
85-98 |
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1:8 |
Average % |
97 |
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Range % |
91-102 |
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Recovery |
The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
|
Serum (n=5) |
86 |
80-94 |
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EDTA plasma (n=4) |
97 |
92-103 |
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Typical Data |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
800 |
2.850 |
2.963 |
2.907 |
2.778 |
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400 |
2.255 |
2.203 |
2.229 |
2.100 |
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200 |
1.279 |
1.256 |
1.268 |
1.139 |
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100 |
0.659 |
0.655 |
0.657 |
0.528 |
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50 |
0.438 |
0.475 |
0.457 |
0.328 |
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25 |
0.256 |
0.242 |
0.249 |
0.120 |
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12.5 |
0.184 |
0.172 |
0.178 |
0.049 |
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0 |
0.126 |
0.132 |
0.129 |
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Troubleshooting
and FAQs |
ELISA kit FAQs |
Storage |
Store at 2-8°C. Please refer to protocol. |
Lead Time |
3-5 working days |