Code | CSB-E08087h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 86 | ||||
Range % | 80-90 | |||||
1:200 | Average % | 93 | ||||
Range % | 88-98 | |||||
1:400 | Average % | 94 | ||||
Range % | 90-100 | |||||
1:800 | Average % | 96 | ||||
Range % | 90-102 |
The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 88-98 | ||||
EDTA plasma (n=4) | 95 | 90-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Human Col Ⅱ ELISA Kit was designed for the quantitative measurement of Human Col Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
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I need to know if the Human Collagen Type Ⅱ, Col Ⅱ ELISA Kit (code CSB-E08087h) detects only native (undenatured) type II collagen, or also denatured type II collagen?
The CSB-E08087h kit has no problem detecting natural Human Collagen Type Ⅱ protein.
For the denatured state, generally speaking, it has an impact on the protein structure, and in theory, it has an impact on the antibody recognition area.