Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit

Code CSB-E08087h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Collagen Type Ⅱ,Col Ⅱ
Alternative Names
Collagen Type 2,Col 2,Collagen Type II,Col II
Abbreviation
Col Ⅱ
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 86  
Range % 80-90  
1:200 Average % 93  
Range % 88-98  
1:400 Average % 94  
Range % 90-100  
1:800 Average % 96  
Range % 90-102  
Recovery
The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 88-98  
EDTA plasma (n=4) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.478 2.387 2.433 2.341  
1000 1.811 1.752 1.782 1.690  
500 1.237 1.142 1.190 1.098  
250 0.863 0.813 0.838 0.746  
125 0.398 0.374 0.386 0.294  
62.5 0.211 0.198 0.205 0.113  
31.25 0.145 0.141 0.143 0.051  
0 0.093 0.090 0.092    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human Col Ⅱ ELISA Kit was designed for the quantitative measurement of Human Col Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

Customer Reviews and Q&A

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 Q&A
Q:

I need to know if the Human Collagen Type Ⅱ, Col Ⅱ ELISA Kit (code CSB-E08087h) detects only native (undenatured) type II collagen, or also denatured type II collagen?

A:

The CSB-E08087h kit has no problem detecting natural Human Collagen Type Ⅱ protein.
For the denatured state, generally speaking, it has an impact on the protein structure, and in theory, it has an impact on the antibody recognition area.

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