Human Collagen alpha-1(II) chain (COL2A1) ELISA kit

Code CSB-EL005739HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P02458
Lead Time 7-14 working days
Abbreviation COL2A1
Target Name collagen, type II, alpha 1
Alias ANFH, AOM, COL11A3, MGC131516, SEDC, cartilage collagen|chondrocalcin|collagen II, alpha-1 polypeptide,Collagen alpha-1(2) chain (COL2A1),Collagen alpha-1(II) chain (COL2A1)
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
HGNC 2200
RGD 2375
MGI 88452
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human COL2A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 104    
Range % 100-107    
1:2 Average % 85    
Range % 81-89    
1:4 Average % 96    
Range % 93-99    
1:8 Average % 88    
Range % 84-93    
Recovery
The recovery of human COL2A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 100 95-105    
EDTA plasma (n=4) 102 98-106    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
20 2.305 2.205 2.255 2.092    
10 1.963 1.924 1.944 1.781    
5 1.614 1.631 1.623 1.460    
2.5 1.204 1.304 1.254 1.091    
1.25 0.782 0.798 0.790 0.627    
0.625 0.463 0.476 0.470 0.307    
0.312 0.306 0.312 0.309 0.146    
0 0.162 0.164 0.163      
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Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Involvement in disease Spondyloepiphyseal dysplasia congenital type (SEDC); Spondyloepiphyseal dysplasia, Stanescu type (SEDSTN); Spondyloepimetaphyseal dysplasia, Strudwick type (SEMDSTWK); Achondrogenesis 2 (ACG2); Legg-Calve-Perthes disease (LCPD); Kniest dysplasia (KD); Avascular necrosis of femoral head, primary, 1 (ANFH1); Osteoarthritis with mild chondrodysplasia (OSCDP); Platyspondylic lethal skeletal dysplasia Torrance type (PLSD-T); Multiple epiphyseal dysplasia with myopia and conductive deafness (EDMMD); Spondyloperipheral dysplasia (SPD); Stickler syndrome 1 (STL1); Stickler syndrome 1 non-syndromic ocular (STL1O); Rhegmatogenous retinal detachment autosomal dominant (DRRD); Czech dysplasia (CZECHD)
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Fibrillar collagen family
Tissue Specificity Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
Database Links

HGNC: 2200

OMIM: 108300

KEGG: hsa:1280

STRING: 9606.ENSP00000369889

UniGene: Hs.408182

Pathway PI3K-Akt signaling pathway
Focal adhesion

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