Human Complement 7,C7 ELISA Kit

Instructions
Code CSB-E11167h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name complement component 7
Alternative Names C7 ELISA Kit; CO7_HUMAN ELISA Kit; complement component 7 ELISA Kit; Complement component C7 ELISA Kit
Abbreviation C7
Uniprot No. P10643
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %91
Range %87-95
1:200Average %95
Range %90-100
1:400Average %97
Range %94-101
1:800Average %94
Range %91-98
Recovery
The recovery of human C7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-94
EDTA plasma (n=4)9993-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.934 2.880 2.907 2.806
5002.102 2.195 2.149 2.048
2501.421 1.504 1.463 1.362
1250.807 0.772 0.790 0.689
62.50.443 0.459 0.451 0.350
31.20.269 0.259 0.264 0.163
15.60.171 0.167 0.169 0.068
00.099 0.103 0.101
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.
Gene References into Functions
  1. this study shows that GG genotype of C7 provides protection against fibrosis severity while showing a higher risk for hepatocellular carcinoma in patients with hepatitis C PMID: 29966690
  2. Two case reports of C7 deficiency leading to recurrent meningitis and other bacterial infections are described. PMID: 28078901
  3. The complement C7 rs6876739 CC genotypes and mannan-binding lectin (MBL2) gene polymorphisms of liver donors were significantly associated with bacterial infection in recipients. PMID: 27063552
  4. Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1 pathway. PMID: 26723877
  5. Borrelial CspA binds the human terminal complement components C7 and C9 and blocks assembly and membrane insertion of the terminal complement complex (TCC). PMID: 23943762
  6. Studies indicate that the deletion defect may be a more commonly distributed cause of C7 deficiency in Ireland. PMID: 22206826
  7. C7 isoelectric focusing variants can determine meningococcal killing in the early stage of infection when antibody-independent killing prevails. PMID: 19931914
  8. To determine transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. PMID: 12595902
  9. The interaction between the factor I domain of C7 and the C345C domain at the C terminus of the C5 alpha-chain plays an essential role in complement membrane attack complex formation and complement lytic activity. PMID: 15879120
  10. recurrence of fulminant meningococcal disease in a complement component C7-deficient patient PMID: 15889368
  11. membrane associated C7 acts as a trap for the late complement components to control excessive inflammation induced by SC5b-9 PMID: 19179470
  12. C7 is associated with multiple sclerosis pathogenesis. PMID: 19221116
  13. Data show that the two C7-FIMs pack closely together with an approximate 2-fold rotational symmetry that is rarely seen in module pairs and has not been observed in FD-containing proteins. PMID: 19419965

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Involvement in disease Complement component 7 deficiency (C7D)
Subcellular Location Secreted
Protein Families Complement C6/C7/C8/C9 family
Database Links

HGNC: 1346

OMIM: 217070

KEGG: hsa:730

STRING: 9606.ENSP00000322061

UniGene: Hs.669878

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