Human Complement 7,C7 ELISA Kit

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Target Background

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Human Complement 7,C7 ELISA Kit

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Protocol

Code	CSB-E11167h-IS
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
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Customer Reviews and Q&A
Target Background

Target Name

complement component 7

Associated names

C7 ELISA Kit; CO7_HUMAN ELISA Kit; complement component 7 ELISA Kit; Complement component C7 ELISA Kit

Abbreviation

Uniprot No.

P10643

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

4.69ng/mL-300ng/mL

Sensitivity

2.345ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:200	Average %	86
	Range %	81-92
1:400	Average %	90
	Range %	85-95
1:800	Average %	93
	Range %	89-98
1:1600	Average %	100
	Range %	96-106

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
300	2.684	2.534	2.609	2.478
150	1.702	1.807	1.754	1.623
75	1.303	1.235	1.269	1.138
37.5	0.840	0.829	0.834	0.703
18.75	0.530	0.522	0.526	0.395
9.38	0.386	0.393	0.389	0.258
4.69	0.262	0.274	0.268	0.137
0	0.135	0.128	0.131

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days

Description

This ELISA kit provides quantitative measurement of Complement 7 (C7) in human samples, supporting research into the membrane attack complex (MAC) and terminal complement pathway activation. Because C7 circulates at microgram-per-milliliter concentrations in healthy serum but shifts meaningfully during complement consumption or deficiency states, this kit is designed to capture both baseline and depleted levels with appropriate sample dilution.

Detection Range: The 4.69–300 ng/mL range requires dilution of serum and plasma samples, where normal C7 levels typically fall between 50–100 µg/mL. This built-in dilution step effectively minimizes matrix interference while still capturing the reduced C7 concentrations seen in complement consumption during bacteremia or immune complex disease.

Sensitivity: At 2.345 ng/mL, the kit detects C7 in diluted serum from individuals with hereditary C7 deficiency or in tissue homogenates where local complement production yields substantially lower concentrations than systemic circulation.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables researchers to correlate systemic C7 levels with local hepatic or extrahepatic C7 expression, critical for studying tissue-specific complement biosynthesis.

Assay Efficiency: The 1–5 hour protocol and 50–100 µL sample volume accommodate longitudinal clinical cohort studies where serial blood draws and high-throughput plate processing are essential.

This kit is particularly suited for investigating meningococcal susceptibility in C7-deficient individuals, MAC-driven tissue injury, and complement biomarker profiling in systemic lupus erythematosus.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor.

Gene References into Functions

this study shows that GG genotype of C7 provides protection against fibrosis severity while showing a higher risk for hepatocellular carcinoma in patients with hepatitis C PMID: 29966690
Two case reports of C7 deficiency leading to recurrent meningitis and other bacterial infections are described. PMID: 28078901
The complement C7 rs6876739 CC genotypes and mannan-binding lectin (MBL2) gene polymorphisms of liver donors were significantly associated with bacterial infection in recipients. PMID: 27063552
Complement proteins C7 and CFH control the stemness of liver cancer cells via LSF-1 pathway. PMID: 26723877
Borrelial CspA binds the human terminal complement components C7 and C9 and blocks assembly and membrane insertion of the terminal complement complex (TCC). PMID: 23943762
Studies indicate that the deletion defect may be a more commonly distributed cause of C7 deficiency in Ireland. PMID: 22206826
C7 isoelectric focusing variants can determine meningococcal killing in the early stage of infection when antibody-independent killing prevails. PMID: 19931914
To determine transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. PMID: 12595902
The interaction between the factor I domain of C7 and the C345C domain at the C terminus of the C5 alpha-chain plays an essential role in complement membrane attack complex formation and complement lytic activity. PMID: 15879120
recurrence of fulminant meningococcal disease in a complement component C7-deficient patient PMID: 15889368
membrane associated C7 acts as a trap for the late complement components to control excessive inflammation induced by SC5b-9 PMID: 19179470
C7 is associated with multiple sclerosis pathogenesis. PMID: 19221116
Data show that the two C7-FIMs pack closely together with an approximate 2-fold rotational symmetry that is rarely seen in module pairs and has not been observed in FD-containing proteins. PMID: 19419965

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Involvement in disease

Complement component 7 deficiency (C7D)

Subcellular Location

Secreted.

Protein Families

Complement C6/C7/C8/C9 family

Database Links

HGNC: 1346

OMIM: 217070

KEGG: hsa:730

STRING: 9606.ENSP00000322061

UniGene: Hs.669878

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Email: [email protected]
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Related pathways

Complement and coagulation cascades

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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