Human Complement C1q tumor necrosis factor-related protein 6(C1QTNF6) ELISA kit

Instructions
Code CSB-EL003651HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name C1q and tumor necrosis factor related protein 6
Alternative Names C1q and tumor necrosis factor related protein 6 ELISA Kit; C1QT6_HUMAN ELISA Kit; C1qtnf6 ELISA Kit; Complement C1q tumor necrosis factor related protein 6 ELISA Kit; Complement C1q tumor necrosis factor-related protein 6 ELISA Kit; CTRP 6 ELISA Kit; CTRP6 ELISA Kit; OTTHUMP00000197772 ELISA Kit; OTTHUMP00000197774 ELISA Kit; PRO1151 ELISA Kit; UNQ581 ELISA Kit; ZACRP6 ELISA Kit
Abbreviation C1QTNF6
Uniprot No. Q9BXI9
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C1QTNF6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %92-101
1:2Average %105
Range %99-108
1:4Average %90
Range %84-94
1:8Average %87
Range %82-91
Recovery
The recovery of human C1QTNF6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9285-96
EDTA plasma (n=4)9082-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.778 2.662 2.720 2.600
101.926 1.905 1.916 1.796
51.315 1.342 1.329 1.209
2.50.703 0.734 0.719 0.599
1.250.390 0.381 0.386 0.266
0.6250.295 0.288 0.292 0.172
0.3120.203 0.209 0.206 0.086
00.119 0.121 0.120
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Gene References into Functions
  1. The results establish CTRP6 as a novel metabolic/immune regulator linking obesity to adipose tissue inflammation and insulin resistance. PMID: 28726640
  2. We found both the rs229527 allele within C1QTNF6 (odds ratio [OR] = 1.23, confidence interval [95% CI]: 1.12-1.33, pAllelic = 4.60 . 10-6) and the rs2284038 allele within RAC2 (OR = 1.10, 95% CI: 1.01-0.19, pAllelic = 3.00 . 10-2) showed significant associations with Graves' Disease susceptibility. PMID: 28665696
  3. CTRP6 expression was decreased in scar tissues and TGF-beta1-treated dermal fibroblasts. CTRP6 inhibits TGF-beta1-induced the proliferation of dermal fibroblasts. PMID: 27155158
  4. CTRP6 inhibits the proliferation and migration of ovarian cancer cells via blocking IL-8/VEGF pathway. PMID: 26648301
  5. CTRP6 is expressed in human synoviocytes, and CTRP6 levels are increased in RA patients. PMID: 26404464
  6. C1qTNF6 is overexpressed and possibly contributes to tumor angiogenesis by activating the Akt pathway in many hepatocellular carcinomas. PMID: 21508531

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Subcellular Location Secreted
Database Links

HGNC: 14343

OMIM: 614910

KEGG: hsa:114904

STRING: 9606.ENSP00000338812

UniGene: Hs.22011

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