Human Complement factor H-related protein 2(CFHR2) ELISA kit

Instructions
Code CSB-EL005275HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name complement factor H-related 2
Alternative Names CFHL2 ELISA Kit; CFHR2 ELISA Kit; Complement factor H related protein 2 ELISA Kit; Complement factor H-related 2 ELISA Kit; Complement factor H-related protein 2 ELISA Kit; DDESK59 ELISA Kit; Factor H-related gene 2 ELISA Kit; FHR 2 ELISA Kit; FHR-2 ELISA Kit; FHR2 ELISA Kit; FHR2_HUMAN ELISA Kit; H factor (complement)-like 3 ELISA Kit; H factor like 3 ELISA Kit; H factor like protein 2 ELISA Kit; H factor-like 3 ELISA Kit; H factor-like protein 2 ELISA Kit; HFL3 ELISA Kit
Abbreviation CFHR2
Uniprot No. P36980
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 ng/mL-400 ng/mL
Sensitivity 1.56 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CFHR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 91
Range % 87-95
1:200 Average % 96
Range % 90-102
1:400 Average % 104
Range % 98-109
1:800 Average % 92
Range % 86-98
Recovery
The recovery of human CFHR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 97-105
EDTA plasma (n=4) 94 87-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
400 2.520 2.637 2.579 2.485
200 2.124 2.205 2.165 2.071
100 1.446 1.557 1.502 1.408
50 0.937 0.923 0.930 0.836
25 0.458 0.461 0.460 0.366
12.5 0.271 0.283 0.277 0.183
6.25 0.156 0.163 0.160 0.066
0 0.093 0.095 0.094  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH. Can associate with lipoproteins and may play a role in lipid metabolism.
Gene References into Functions
  1. Studies indicate an association between CFHR2-rs2986127 and acute anterior uveitis (AAU) diagnosis. PMID: 27306586
  2. Next-generation sequencing of the CFH region identified putatively functional variants (missense, splice site and indel) on the four common haplotypes. We found no expression of any of the five CFH-related genes in the retina or RPE/Choroid/Sclera, in contrast to the liver, which is the main source of the circulating proteins. [CFHR2] PMID: 27196323
  3. Thus CFHR2 likely acts in concert with factor H, as CFHR2 inhibits convertases while simultaneously allowing factor H assisted degradation by factor I. PMID: 24260121
  4. A hybrid CFHR2-CFHR5 plasma protein, arising from a chromosomal deletion mutation stabilizes the C3 convertase and reduces factor H-mediated convertase decay. PMID: 24334459
Subcellular Location Secreted
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

HGNC: 4890

OMIM: 600889

KEGG: hsa:3080

STRING: 9606.ENSP00000356385

UniGene: Hs.632450

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