Human Complement fragment 4b,C4b ELISA Kit

Instructions
Code CSB-E09302h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name Complement fragment 4b,C4b
Alternative Names Basic complement C4 ELISA Kit; C2B5 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4b ELISA Kit; C4B1 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; CO4B_HUMAN ELISA Kit; Complement C4 gamma chain ELISA Kit; Complement component 4B ELISA Kit; CPAMD3 ELISA Kit
Abbreviation C4b
Uniprot No. P0C0L5
Species Homo sapiens (Human)
Sample Types serum, plasma
Detection Range 15.6 ng/mL-1000 ng/mL
Sensitivity 3.9 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human C4b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:200Average %84
Range %80-94
1:400Average %97
Range %91-102
1:800Average %98
Range %92-105
1:1600Average %92
Range %86-99
Recovery
The recovery of human C4b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-98
EDTA plasma (n=4)9890-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
10002.566 2.366 2.466 2.373
5002.031 1.931 1.981 1.888
2501.409 1.319 1.364 1.271
1250.867 0.854 0.861 0.768
62.50.524 0.514 0.519 0.426
31.20.345 0.325 0.335 0.242
15.60.213 0.203 0.208 0.115
00.094 0.091 0.093  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.; FUNCTION
Involvement in disease Systemic lupus erythematosus (SLE); Complement component 4B deficiency (C4BD)
Subcellular Location Secreted, Cell junction, synapse, Cell projection, axon, Cell projection, dendrite
Tissue Specificity Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland,and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra
Database Links

HGNC: 1324

OMIM: 120820

KEGG: hsa:100293534

STRING: 9606.ENSP00000415941

UniGene: Hs.534847

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1