Human Complement fragment 4b,C4b ELISA Kit

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Human Complement fragment 4b,C4b ELISA Kit

Name: Human Complement fragment 4b,C4b ELISA Kit
Brand: CUSABIO
SKU: CSB-E09302h

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Code	CSB-E09302h
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Target Name

Complement fragment 4b,C4b

Alternative Names

Basic complement C4 ELISA Kit; C2B5 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4b ELISA Kit; C4B1 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; CO4B_HUMAN ELISA Kit; Complement C4 gamma chain ELISA Kit; Complement component 4B ELISA Kit; CPAMD3 ELISA Kit

Abbreviation

C4b

Uniprot No.

P0C0L5

Species

Homo sapiens (Human)

Sample Types

serum, plasma

Detection Range

15.6 ng/mL-1000 ng/mL

Sensitivity

3.9 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human C4b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	84
	Range %	80-94
1:400	Average %	97
	Range %	91-102
1:800	Average %	98
	Range %	92-105
1:1600	Average %	92
	Range %	86-99

Recovery

The recovery of human C4b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-98
EDTA plasma (n=4)	98	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.566	2.366	2.466	2.373
500	2.031	1.931	1.981	1.888
250	1.409	1.319	1.364	1.271
125	0.867	0.854	0.861	0.768
62.5	0.524	0.514	0.519	0.426
31.2	0.345	0.325	0.335	0.242
15.6	0.213	0.203	0.208	0.115
0	0.094	0.091	0.093

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Traumatic brain injury increases plasma astrocyte‐derived exosome levels of neurotoxic complement proteins Goetzl EJ,FASEB,2020
High complement levels in astrocyte-derived exosomes of Alzheimer's disease Goetzl EJ.et al,Ann Neurol,2018
Serum complement C4b, fibronectin, and prolidase are associated with the pathological changes of pulmonary tuberculosis Wang C et al,BMC Infect Dis,2014

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Function	Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.; FUNCTION
Involvement in disease	Systemic lupus erythematosus (SLE); Complement component 4B deficiency (C4BD)
Subcellular Location	Secreted, Cell junction, synapse, Cell projection, axon, Cell projection, dendrite
Tissue Specificity	Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland,and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra
Database Links	HGNC: 1324 OMIM: 120820 KEGG: hsa:100293534 STRING: 9606.ENSP00000415941 UniGene: Hs.534847

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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