Human Cross Linked C-Telopeptide Of Type II Collagen ,CTX-Ⅱ ELISA Kit

Instructions
Code CSB-E14328h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Cross Linked C-Telopeptide Of Type II Collagen ,CTX-
Alternative Names Cross Linked C-Telopeptide Of Type 2 Collagen ,CTX-2,Cross Linked C-Telopeptide Of Type II Collagen ,CTX-Ⅱ
Abbreviation CTX-
Species Homo sapiens (Human)
Sample Types serum, plasma, urine
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-98  
1:2 Average % 100  
Range % 96-104  
1:4 Average % 99  
Range % 92-105  
1:8 Average % 92  
Range % 87-98  
Recovery
The recovery of human CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 90-100  
EDTA plasma (n=4) 100 95-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.462 2.671 2.567 2.423  
10 1.959 1.964 1.962 1.818  
5 1.398 1.345 1.372 1.228  
2.5 0.822 0.837 0.830 0.686  
1.25 0.546 0.558 0.552 0.408  
0.625 0.382 0.396 0.389 0.245  
0.312 0.281 0.297 0.289 0.145  
0 0.142 0.146 0.144    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

I want an citation/publication about Human Cross Linked C-Telopeptide Of Type II Collagen ,CTX-2 ELISA Kit CSB-E14328h in urine samples. Do you have any papers?

A:
Thanks for your inquiry.
This kit can be used to test urine samples.
We tested human normal urine samples before and the test value is 0.3--1.25ng/ml. Some customers said the detection rate is very low. I will send you the manual and published reference by e-mail to you soon.Pls let me know if you have any further questions. Thank you.
 Customer Reviews

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