Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

Instructions
Code CSB-E11224h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I
Alternative Names Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1,Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
Abbreviation CTX-I
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 83-95  
1:2 Average % 95  
Range % 91-98  
1:4 Average % 97  
Range % 90-103  
1:8 Average % 96  
Range % 91-101  
Recovery
The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 81-92  
EDTA plasma (n=4) 96 93-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.995 2.960 2.978 2.838  
20 2.557 2.570 2.564 2.424  
10 1.666 1.765 1.716 1.576  
5 0.839 0.886 0.863 0.723  
2.5 0.491 0.524 0.508 0.368  
1.25 0.263 0.251 0.257 0.117  
0.625 0.201 0.212 0.207 0.067  
0 0.138 0.142 0.140    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Please provide the following information regarding Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit CSB-E11224h
a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. B208. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
CSB-E11224h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is synthetic peptide.
Pls let me know if you have any further questions. Thank you.

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