Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

Code CSB-E11720h
Size 96T,5×96T,10×96T
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Product Details

Target Name
lactate dehydrogenase D
Alternative Names
D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit
Abbreviation
LDHD
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 mIU/mL-2000 mIU/mL
Sensitivity
7.8 mIU/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %87-103
1:2Average %91
Range %86-103
1:4Average %94
Range %86-105
1:8Average %91
Range %84-100
Recovery
The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9791-106
EDTA plasma (n=4)9686-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mIU/mlOD1OD2AverageCorrected
20002.418 2.385 2.402 2.265
10001.773 1.728 1.751 1.614
5000.936 1.002 0.969 0.832
2500.624 0.651 0.638 0.501
1250.407 0.422 0.415 0.278
62.50.282 0.287 0.285 0.148
31.250.253 0.264 0.259 0.122
00.135 0.139 0.137
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human LDHD ELISA Kit was designed for the quantitative measurement of Human LDHD protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 mIU/mL-2000 mIU/mL and the sensitivity is 7.8 mIU/mL.

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Target Background

Function
(From Uniprot)
Involved in D-lactate, but not L-lactate catabolic process.
Gene References into Functions
  1. The activity and expression of the mitochondrial D-lactate dehydrogenase, is higher in prostate cancer versus normal prostate cells. PMID: 23333299
  2. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
  3. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
Subcellular Location
Mitochondrion.
Protein Families
FAD-binding oxidoreductase/transferase type 4 family
Tissue Specificity
Expressed moderately in heart and liver and at lower levels in skeletal muscle and kidney.
Database Links

HGNC: 19708

OMIM: 607490

KEGG: hsa:197257

STRING: 9606.ENSP00000300051

UniGene: Hs.380929

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