Product Details

Target Name

defensin, alpha 6, Paneth cell-specific

Alternative Names

alpha 6 ELISA Kit; DEF6 ELISA Kit; DEF6_HUMAN ELISA Kit; DEFA 6 ELISA Kit; DEFA6 ELISA Kit; Defensin 6 ELISA Kit; Defensin alpha 6 ELISA Kit; Defensin alpha 6 Paneth cell specific ELISA Kit; Defensin ELISA Kit; Defensin-6 ELISA Kit; HD 6 ELISA Kit; HD6 ELISA Kit

Abbreviation

DEFA6

Uniprot No.

Q01524

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	103
	Range %	98-106
1:4	Average %	110
	Range %	106-114
1:8	Average %	92
	Range %	89-97

Recovery

The recovery of human DEFA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	97	93-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.217	2.104	2.161	2.047
5	1.762	1.752	1.757	1.643
2.5	1.253	1.198	1.226	1.112
1.25	0.849	0.856	0.853	0.739
0.625	0.528	0.534	0.531	0.417
0.312	0.354	0.368	0.361	0.247
0.156	0.244	0.238	0.241	0.127
0	0.115	0.112	0.114

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human DEFA6 ELISA Kit was designed for the quantitative measurement of Human DEFA6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Related Products

Target Background

Function
(From Uniprot)

Has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. May protect cells against infection with HIV-1.

Gene References into Functions

HD6 assembled into nanonets can prevent key steps in C. albicans pathogenesis PMID: 28198610
alpha-Defensin 6 Self-Assembly Prevents Adhesion and Suppresses Virulence Traits of Candida albicans PMID: 28026958
Downregulation of HD6 in noninflamed mucosa may contribute to mucosal barrier dysfunction of patients with Crohn's Disease. PMID: 26891258
Paneth cell alpha-defensin 6 is an antimicrobial peptide. PMID: 25354318
Native HD-6 readily self-assembles into elongated fibrils observable by transmission electron microscopy, agglutinates both Gram-negative and -positive bacteria, and prevents the Listeria monocytogenes from invading cultured cells. PMID: 25158166
In the meta-analysis, DEFA6 rs13275170 and DEFB1 rs2738169 had both a 1.3-fold increased odds ratio for gastric cancer. PMID: 23028900
study reports that HD6 affords protection against invasion by enteric bacterial pathogens in vitro and in vivo; after stochastic binding to bacterial surface proteins, HD6 undergoes ordered self-assembly to form fibrils and nanonets that surround and entangle bacteria PMID: 22722251
Regions 2, 3 & 9, 10 were more hydrophobic than other regions. It was difficult for the HD molecule to have many conformations because of its 3 S-S bridges; however, it responds to various external factors by restricting conformation. PMID: 21873175
Defensin alpha 6 (DEFA 6) may have a role in adenoma but not in progression to colon carcinoma PMID: 20979654
defensin alpha6 is highly expressed in colon cancer cell lines PMID: 15613481
Crystal structure of HD6. PMID: 17088326
Human enteric defensin (HD)-5 and HD-6 were detected in Paneth cells, which are observed in the gastric metaplastic mucosa as well as small intestinal epithelia. PMID: 19250512

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Subcellular Location

Secreted.

Protein Families

Alpha-defensin family

Tissue Specificity

Paneth cells of the small intestine.

Database Links

HGNC: 2765

OMIM: 600471

KEGG: hsa:1671

STRING: 9606.ENSP00000297436

UniGene: Hs.711

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Code	CSB-EL006658HU
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Human Defensin-6(DEFA6) ELISA kit

Product Details

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Target Background