Human Defensin-6(DEFA6) ELISA kit

Code CSB-EL006658HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name defensin, alpha 6, Paneth cell-specific
Alternative Names alpha 6 ELISA Kit; DEF6 ELISA Kit; DEF6_HUMAN ELISA Kit; DEFA 6 ELISA Kit; DEFA6 ELISA Kit; Defensin 6 ELISA Kit; Defensin alpha 6 ELISA Kit; Defensin alpha 6 Paneth cell specific ELISA Kit; Defensin ELISA Kit; Defensin-6 ELISA Kit; HD 6 ELISA Kit; HD6 ELISA Kit
Abbreviation DEFA6
Uniprot No. Q01524
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 91-99
1:2 Average % 103
Range % 98-106
1:4 Average % 110
Range % 106-114
1:8 Average % 92
Range % 89-97
The recovery of human DEFA6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 84 81-89
EDTA plasma (n=4) 97 93-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.217 2.104 2.161 2.047
5 1.762 1.752 1.757 1.643
2.5 1.253 1.198 1.226 1.112
1.25 0.849 0.856 0.853 0.739
0.625 0.528 0.534 0.531 0.417
0.312 0.354 0.368 0.361 0.247
0.156 0.244 0.238 0.241 0.127
0 0.115 0.112 0.114  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. May protect cells against infection with HIV-1.
Gene References into Functions
  1. HD6 assembled into nanonets can prevent key steps in C. albicans pathogenesis PMID: 28198610
  2. alpha-Defensin 6 Self-Assembly Prevents Adhesion and Suppresses Virulence Traits of Candida albicans PMID: 28026958
  3. Downregulation of HD6 in noninflamed mucosa may contribute to mucosal barrier dysfunction of patients with Crohn's Disease. PMID: 26891258
  4. Paneth cell alpha-defensin 6 is an antimicrobial peptide. PMID: 25354318
  5. Native HD-6 readily self-assembles into elongated fibrils observable by transmission electron microscopy, agglutinates both Gram-negative and -positive bacteria, and prevents the Listeria monocytogenes from invading cultured cells. PMID: 25158166
  6. In the meta-analysis, DEFA6 rs13275170 and DEFB1 rs2738169 had both a 1.3-fold increased odds ratio for gastric cancer. PMID: 23028900
  7. study reports that HD6 affords protection against invasion by enteric bacterial pathogens in vitro and in vivo; after stochastic binding to bacterial surface proteins, HD6 undergoes ordered self-assembly to form fibrils and nanonets that surround and entangle bacteria PMID: 22722251
  8. Regions 2, 3 & 9, 10 were more hydrophobic than other regions. It was difficult for the HD molecule to have many conformations because of its 3 S-S bridges; however, it responds to various external factors by restricting conformation. PMID: 21873175
  9. Defensin alpha 6 (DEFA 6) may have a role in adenoma but not in progression to colon carcinoma PMID: 20979654
  10. defensin alpha6 is highly expressed in colon cancer cell lines PMID: 15613481
  11. Crystal structure of HD6. PMID: 17088326
  12. Human enteric defensin (HD)-5 and HD-6 were detected in Paneth cells, which are observed in the gastric metaplastic mucosa as well as small intestinal epithelia. PMID: 19250512

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Subcellular Location Secreted
Protein Families Alpha-defensin family
Tissue Specificity Paneth cells of the small intestine.
Database Links

HGNC: 2765

OMIM: 600471

KEGG: hsa:1671

STRING: 9606.ENSP00000297436

UniGene: Hs.711


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