Human Dehydroepiandrosterone sulfate,DHEA-S ELISA Kit

Code CSB-E05105h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Dehydroepiandrosterone sulfate,DHEA-S
Abbreviation
DHEA-S
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
20 ng/mL-4000 ng/mL
Sensitivity
10 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DHEA-S in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 90    
Range % 85-103    
1:2 Average % 96    
Range % 89-105    
1:4 Average % 94    
Range % 90-100    
1:8 Average % 90    
Range % 83-110    
Recovery
The recovery of human DHEA-S spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 86-103    
EDTA plasma (n=4) 89 84-93    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average      
0 1.953 1.917 1.935      
20 1.321 1.339 1.330      
80 0.843 0.855 0.849      
320 0.511 0.530 0.521      
1000 0.329 0.324 0.327      
4000 0.189 0.184 0.187      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human DHEA-S ELISA Kit was designed for the quantitative measurement of Human DHEA-S protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 20 ng/mL-4000 ng/mL and the sensitivity is 10 ng/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X