Human Dipeptidase 1(DPEP1) ELISA kit

Code CSB-EL007123HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
dipeptidase 1 (renal)
Alternative Names
Dehydropeptidase-I ELISA Kit; Dipeptidase 1 (renal) ELISA Kit; Dipeptidase 1 ELISA Kit; DPEP1 ELISA Kit; DPEP1_HUMAN ELISA Kit; hRDP ELISA Kit; MBD1 ELISA Kit; MDP ELISA Kit; Microsomal dipeptidase ELISA Kit; RDP ELISA Kit; Renal dipeptidase ELISA Kit
Abbreviation
DPEP1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cancer
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DPEP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %88
Range %80-93
1:2Average %97
Range %91-106
1:4Average %102
Range %92-111
1:8Average %94
Range %86-99
Recovery
The recovery of human DPEP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9890-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
15001.885 1.752 1.819 1.739
7501.303 1.223 1.263 1.183
3750.812 0.778 0.795 0.715
187.50.465 0.453 0.459 0.379
940.331 0.319 0.325 0.245
470.194 0.191 0.193 0.113
23.50.129 0.126 0.128 0.048
00.080 0.079 0.080  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human DPEP1 ELISA Kit was designed for the quantitative measurement of Human DPEP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

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Target Background

Function
(From Uniprot)
Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4. Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation. Possesses also beta lactamase activity and hydrolytically inactivates beta-lactam antibiotics.; Independently of its dipeptidase activity acts as an adhesion receptor for neutrophils recruitement from bloodstream into inflammed lungs and liver.
Gene References into Functions
  1. The results suggest that DPEP1 promotes cancer metastasis by regulating E-cadherin plasticity and that it might be a potential therapeutic target for preventing the progression of colon cancer. PMID: 26824987
  2. In this study, we present an analysis of Neanderthal introgression at the dipeptidase 1 gene, DPEP1. PMID: 26392408
  3. Dipeptidase 1 has been identified as an excellent marker of high-grade IEN and CRC, and may thus be applied for screening of early neoplastic lesions and for prognostic stratification. PMID: 23839495
  4. DPEP1 plays a role in pancreatic cancer aggressiveness. PMID: 22363658
  5. DPEP1 is expressed in the early stages of colon carcinogenesis and affects cancer cell invasiveness. PMID: 20824289
  6. we identified a novel immunohistochemical marker, dipeptidase 1, to distinguish primary mucinous ovarian cancers from ovarian metastasis of colorectal cancers. PMID: 21076463
  7. Crystal structure of human renal dipeptidase involved in beta-lactam hydrolysis PMID: 12144777
  8. DPEP1 has a role in colorectal carcinoma PMID: 15145522

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Subcellular Location
Apical cell membrane; Lipid-anchor, GPI-anchor. Cell projection, microvillus membrane; Lipid-anchor, GPI-anchor.
Protein Families
Metallo-dependent hydrolases superfamily, Peptidase M19 family
Tissue Specificity
Expressed in lung and kidneys.
Database Links

HGNC: 3002

OMIM: 179780

KEGG: hsa:1800

STRING: 9606.ENSP00000261615

UniGene: Hs.109

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