Human Endostatin,ES ELISA Kit

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Human Endostatin,ES ELISA Kit

Name: Human Endostatin,ES ELISA Kit
Brand: CUSABIO
SKU: CSB-E07973h

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Citations (3) Protocol MSDS

Code	CSB-E07973h
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (3)
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Target Name

Endostatin,ES

Alternative Names

FLJ27325, FLJ34914, KNO, KNO1, MGC74745, alpha 1 type XVIII collagen|antiangiogenic agent|endostatin|multi-functional protein MFP

Abbreviation

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

15.6 pg/ml -1000 pg/ml

Sensitivity

3.9 pg/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:200	Average %	87
	Range %	80-94
1:400	Average %	93
	Range %	88-100
1:800	Average %	92
	Range %	86-100
1:1600	Average %	90
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

1000

1.976

2.034

2.005

1.827

500

1.120

1.167

1.143

0.966

250

0.751

0.728

0.740

0.562

125

0.494

0.519

0.506

0.329

62.5

0.337

0.321

0.329

0.151

31.2

0.242

0.253

0.247

0.070

15.6

0.221

0.214

0.218

0.040

0.180

0.175

0.178

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The product CSB-E07973h is a sandwich ELISA kit developed to measure levels of human endostatin (ES) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of endostatin bound in the initial step.

Endostatin, a 20-kDa C-terminal cleavage product of type XVIII collagen, is an endogenous inhibitor known for its ability to inhibit the proliferation and migration of endothelial cells, angiogenesis, and tumor growth. Studies have proved that circulating endostatin increases significantly in patients with kidney and heart failure and may also contribute to disease progression. Endostatin inhibits angiogenesis by directly binding to both VEGFR1 and VEGFR2 and blocking VEGF interaction with Flt-1 and Flk-1 to prevent VEGF-induced tyrosine phosphorylation of VEGFR1 and VEGFR2 and all downstream signaling events.

Intelligent drugs based on notch protein remodeling: a defensive targeting strategy for tumor therapy Y Sun, Y Lu, X Li, Y He, TK Yong, CS Keng,cell death & disease,2024
Effects of upper- and lower-extremity resistance training on serum vascular endothelial growth factor, myostatin, endostatin and follistatin levels in sedentary male students P Farzanegi,Science & Sports,2020
Correlation of Plasma Vascular Endothelial Growth Factor and Endostatin Levels with Symptomatic Intra-and Extracranial Atherosclerotic Stenosis in a Chinese Han Yu F.et al,J Stroke Cerebrovasc Dis. ,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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