Human Endothelial lipase,EL ELISA Kit

Instructions
Code CSB-E08217h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name lipase, endothelial
Alternative Names EDL ELISA Kit; EL ELISA Kit; Endothelial cell-derived lipase ELISA Kit; Endothelial lipase ELISA Kit; Endothelial lipase precursor ELISA Kit; Lipase endothelial ELISA Kit; LIPE_HUMAN ELISA Kit; LIPG ELISA Kit; Lipoprotein lipase H ELISA Kit; PRO719 ELISA Kit
Abbreviation LIPG
Uniprot No. Q9Y5X9
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 83-102  
1:2 Average % 90  
Range % 85-102  
1:4 Average % 93  
Range % 89-99  
1:8 Average % 94  
Range % 87-108  
Recovery
The recovery of human EL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 86-104  
EDTA plasma (n=4) 95 89-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.415 2.386 2.401 2.271  
20 1.702 1.734 1.718 1.588  
10 1.204 1.195 1.200 1.070  
5 0.730 0.711 0.721 0.591  
2.5 0.417 0.429 0.423 0.293  
1.25 0.296 0.285 0.291 0.161  
0.625 0.234 0.250 0.242 0.112  
0 0.127 0.132 0.130    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
Gene References into Functions
  1. the LIPG polymorphism (584 C/T) - CAD correlation study in Turkish people living in Turkey. Results of this study suggest that the CC genotype may be a genetic risk factor for CAD and carrying T allele may be protective against CAD. PMID: 30150432
  2. We identified a missense Asn396Ser mutation (rs77960347) in the endothelial lipase (LIPG) gene, occurring with an allele frequency of 1% in the general population, which was significantly associated with depressive symptoms (P-value=5.2 x 10-08, beta=7.2). PMID: 27431295
  3. the EL 2237 A allele might be associated with an increased Apo A1 level in Coronary Artery Disease subjects. PMID: 27612170
  4. Increased hepatic expression of endothelial lipase attenuates cholesterol diet-induced hypercholesterolemia and protects against atherosclerosis. PMID: 28546217
  5. Endothelial lipase is upregulated in human umbilical vein endothelial cells by interleukin-6 partly via the p38 MAPK and p65 NF-kappaB signaling pathways. PMID: 27430252
  6. Endothelial lipase (EL) 2037T/C and 2237 G/A polymorphisms might not affect the lipid-owing effects of rosuvastatin in Chinese coronary artery disease patients PMID: 27600285
  7. Endothelial lipase protein expression was increased in skeletal muscle of middle-aged men with high oxygen consumption. PMID: 26447519
  8. LIPG Polymorphisms are associated with Hyperlipidemia. PMID: 27590083
  9. FoxA1, FoxA2, and LIPG control the uptake of extracellular lipids for breast cancer growth. PMID: 27045898
  10. results suggested that the SNP -384A/C in the LIPG gene may be associated with risk for coronary artery disease(CAD)and the LIPG gene may play a role in CAD in the Han Chinese PMID: 26124511
  11. Studied if a specific variant of the endothelial lipase gene was more specifically linked to the severity of diabetic retinopathy. PMID: 24852509
  12. Several lipid-related gene polymorphisms interact with overweight/obesity to modulate blood pressure levels. PMID: 23109900
  13. EL-384A/C polymorphism was significantly associated with the ACS and lipids profile in an elderly Uygur population in Xinjiang. PMID: 25291260
  14. Results showed that the protein levels of endothelial lipase (EL), NF-kappaB p65, MAPK p38 protein levels, in addition to the proliferation of umbilical vein endothelial cells (HUVECs), were increased by angiotensin II (AngII). PMID: 25250890
  15. Genetic studies of patients with early onset of coronary artery disease (CAD) in the Chinese Han population show that the EL 584C/T variant is not always involved in the pathogenesis of early-onset CAD. PMID: 24634127
  16. The results of the present meta-analysis suggest that the carriers of EL 584 T allele have a higher HDL-C level in Caucasian populations. Whereas, it might not be a protective factor for CHD. PMID: 24886585
  17. Results indicate the spatial consensus catalytic triad "Ser-Asp-His", a characteristic motif in lipoprotein lipase, hepatic lipase, and endothelial lipase. PMID: 23991054
  18. EL activity was higher in metabolic syndrome and in obesity. It was negatively associated with high-density lipoprotein-cholesterol and apolipoprotein A-I in control and metabolic syndrome, respectively. PMID: 24458708
  19. T allele of LIPG -584C/T polymorphism might play a potential role in the susceptibility to atherogenesis in the Turkish population. PMID: 23673478
  20. Interleukin-6 stimulates the translocation of HDL through the endothelium, the first step in reverse cholesterol transport pathway, by enhancing EL expression. PMID: 24115033
  21. Complete loss-of-function mutation of LIPG had higher plasma high-density cholesterol levels compared with partial loss-of-function mutation carriers. PMID: 23243195
  22. the EL -384A/C gene polymorphism might be associated with ACS in Chinese Han population, suggesting that the variant might be involved in the pathogenesis of ACS. PMID: 22723003
  23. Urinary endothelial lipase is a highly accurate gastric cancer biomarker that is potentially applicable to the general screening with high sensitivity and specificity. PMID: 23510199
  24. Endothelial lipase supplies cells with free fatty acids and HDL-derived lysophosphatidylcholine and lysophosphatidylethanolamine species resulting in increased cellular triglycerides and phosphatidylcholine (PC) content and decreased PC synthesis. PMID: 23075452
  25. EL expression modulates vascular remodeling as well as plasma HDL-C levels PMID: 22972429
  26. Starvation regulates endothelial lipase expression via SREBP-2. PMID: 23102786
  27. analysis of LIPG, CETP, and GALNT2 mutations in Caucasian families with extremely high HDL cholesterol PMID: 22952570
  28. study tested the hypothesis that placental EL expression is affected by obesity in pregnancy and gestational diabetes; metabolic inflammation with high leptin and increased TNF-alpha concentration at fetal-placental interface regulates placental EL expression PMID: 21852675
  29. Endothelial lipase plays a role in thyroid and adipocyte biology in addition to its well-known role in endothelial function and HDL metabolism. PMID: 22740344
  30. Common and rare genetic variants in CETP and LIPC, but not LIPG, were more commonly found in the Thai HALP group, which could potentially contribute to high high-density lipoprotein cholesterol phenotypes in this population. PMID: 22464213
  31. a reduction in nascent HDL formation may be partly responsible for reduced HDL-C during inflammation when both EL and SAA are known to be upregulated PMID: 21957202
  32. we found that a common nonfunctional coding variant associated with HDL-C (rs2000813) is in linkage disequilibrium with a 5' UTR variant (rs34474737) that decreases LIPG promoter activity PMID: 22174694
  33. Treatment of patients with coronary artery disease with lipid-modulation and/or antiplatelet drug may significantly decrease the expression of endothelial lipase. PMID: 21122200
  34. results suggest that the subjects with TT endothelial lipase genotype benefit more from alcohol consumption than the subjects with CT and CC genotypes in increasing serum high-density lipoprotein cholesterol and apolipoprotein (Apo) AI levels PMID: 21816559
  35. The c.584C>T EL polymorphism is associated with a higher risk of diabetic retinopathy that could be linked to modifications in HDL-cholesterol metabolism and blood pressure levels PMID: 21145773
  36. Endothelial lipase and EL-generated lysophosphatidylcholines promote endothelial IL-8 synthesis. PMID: 21130993
  37. Sulforaphane inhibits endothelial lipase expression via inhibition of NF-kappaB which may have a beneficial effect on HDL cholesterol levels PMID: 20688330
  38. The LIPG 584T allele is associated with increased serum HDL-C, TC and ApoB levels. PMID: 20923576
  39. common genetic variation in endothelial lipase (LIPG) does not appear to have a role in risk for coronary artery disease and deep venous thrombosis PMID: 20466371
  40. Statins can reduce EL expression in vitro and in vivo via inhibition of RhoA activity. The inhibition of EL expression in the vessel wall may contribute to the anti-atherogenic effects of statins. PMID: 20045866
  41. LPL and LIPG are reported in the human testis and in germ cell neoplasms. PMID: 19780863
  42. It seems that plasma endothelial lipase levels in individuals with atherosclerosis might be higher than that measured in healthy individuals. [review] PMID: 20621031
  43. Thus, this N-terminal variant results in reduced secretion of endothelial lipase, plausibly leading to increased HDL-C levels. PMID: 19411705
  44. EDL mediates both HDL binding and uptake, and the selective uptake of HDL-CE, independently of lipolysis and CLA-1. PMID: 12164779
  45. Endothelial lipase has a role in HDL metabolism [review] PMID: 12569156
  46. EL is a major determinant of HDL concentration in humans PMID: 12601178
  47. Expression of human endothelial lipase in mice results in dose-dependent increase in postheparin plasma phospholipase activity, catabolic rate of HDL-apolipoprotein, and uptake of apoA-I in both kidney and liver PMID: 14517167
  48. Hepatic expression of human EL in mice resulted in markedly decreased levels of VLDL/LDL cholesterol, phospholipid, and apoB accompanied by significantly increased LDL apolipoprotein and phospholipid catabolism. PMID: 15117821
  49. N-linked glycosylation has a role in the secretion and activity of endothelial lipase PMID: 15342690
  50. results suggest that endothelial lipase (EL) on the endothelial cell surface can promote monocyte adhesion to the vascular endothelium through the interaction with heparan sulfate proteoglycans PMID: 15485805

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Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Tissue Specificity High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary. Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not
Database Links

HGNC: 6623

OMIM: 603684

KEGG: hsa:9388

STRING: 9606.ENSP00000261292

UniGene: Hs.465102

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