Human Epigen(EPGN) ELISA kit

Code CSB-EL007719HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
epithelial mitogen homolog (mouse)
Alternative Names
ALGV3072 ELISA Kit; EPG ELISA Kit; EPGN ELISA Kit; EPGN_HUMAN ELISA Kit; Epigen ELISA Kit; Epigen precursor ELISA Kit; Epithelial mitogen ELISA Kit; PRO9904 ELISA Kit
Abbreviation
EPGN
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 103    
Range % 96-107    
1:2 Average % 102    
Range % 97-105    
1:4 Average % 92    
Range % 85-96    
1:8 Average % 97    
Range % 92-101    
Recovery
The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 92-103    
EDTA plasma (n=4) 105 98-109    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
100 2.687 2.568 2.628 2.528    
50 2.143 2.002 2.073 1.973    
25 1.298 1.277 1.288 1.188    
12.5 0.732 0.705 0.719 0.619    
6.25 0.414 0.393 0.404 0.304    
3.12 0.263 0.252 0.258 0.158    
1.56 0.172 0.168 0.170 0.070    
0 0.101 0.099 0.100      
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Customer Reviews and Q&A

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 Q&A
Q:

Are there any recombinant proteins in this kit?
If so, what are the host cells?
Are there any components containing azide, thimerosal or 2-ME?
What is the composition of the Stop solution?

A:
Thanks for your inquiry!
Pls check our information:
Standard: CHO cell-derived recombinant protein
There is no component containing azide, thimerosal or 2-ME with this kit.
Stop solution: 4N sulfuric acid

Target Background

Function
(From Uniprot)
Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
Gene References into Functions
  1. Study shows how the EGFR ligands epiregulin (EREG) and epigen (EPGN) stabilize different dimeric conformations of the EGFR extracellular region. Results reveal how responses to different EGFR ligands are defined by receptor dimerization strength and signaling dynamics. These findings have broad implications for understanding receptor tyrosine kinase (RTK) signaling specificity. PMID: 28988771
  2. cloning of a related gene in mouse PMID: 112783
  3. EPG is an EGF-like growth factor and a low affinity ligand PMID: 15611079
Subcellular Location
[Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Secreted.
Database Links

HGNC: 17470

KEGG: hsa:255324

UniGene: Hs.401237

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