Human Epigen(EPGN) ELISA kit

Code CSB-EL007719HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q6UW88
Lead Time 7-14 working days
Abbreviation EPGN
Target Name epithelial mitogen homolog (mouse)
Alias UNQ3072, ALGV3072, EPG, FLJ75542, PRO9904, epigen, epithelial mitogen
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
HGNC 17470
RGD 1560084
MGI 1919170
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 103    
Range % 96-107    
1:2 Average % 102    
Range % 97-105    
1:4 Average % 92    
Range % 85-96    
1:8 Average % 97    
Range % 92-101    
Recovery
The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 92-103    
EDTA plasma (n=4) 105 98-109    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
100 2.687 2.568 2.628 2.528    
50 2.143 2.002 2.073 1.973    
25 1.298 1.277 1.288 1.188    
12.5 0.732 0.705 0.719 0.619    
6.25 0.414 0.393 0.404 0.304    
3.12 0.263 0.252 0.258 0.158    
1.56 0.172 0.168 0.170 0.070    
0 0.101 0.099 0.100      
References
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Function Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
Subcellular Location Isoform 1: Membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 3: Secreted, SUBCELLULAR LOCATION: Isoform 4: Secreted, SUBCELLULAR LOCATION: Isoform 5: Secreted, SUBCELLULAR LOCATION: Isoform 6: Secreted
Database Links

HGNC: 17470

KEGG: hsa:255324

UniGene: Hs.401237

Q&A and Customer Reviews

Q&A
Q:

Are there any recombinant proteins in this kit?
If so, what are the host cells?
Are there any components containing azide, thimerosal or 2-ME?
What is the composition of the Stop solution?

A:
Thanks for your inquiry!
Pls check our information:
Standard: CHO cell-derived recombinant protein
There is no component containing azide, thimerosal or 2-ME with this kit.
Stop solution: 4N sulfuric acid
Customer Reviews

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