Human Epoxide hydrolase 2(EPHX2) ELISA kit

Code CSB-EL007735HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
epoxide hydrolase 2, cytoplasmic
Alternative Names
Bifunctional epoxide hydrolase 2 ELISA Kit; CEH ELISA Kit; Cytosolic epoxide hydrolase ELISA Kit; EPHX2 ELISA Kit; Epoxide hydratase ELISA Kit; Epoxide hydrolase 2 ELISA Kit; Epoxide hydrolase 2 cytoplasmic ELISA Kit; epoxide hydrolase 2, cytosolic ELISA Kit; Epoxide hydrolase soluble ELISA Kit; HYES_HUMAN ELISA Kit; SEH ELISA Kit; Soluble epoxide hydrolase ELISA Kit
Abbreviation
EPHX2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.44 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human EPHX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 84-93
1:2 Average % 97
Range % 92-101
1:4 Average % 91
Range % 85-95
1:8 Average % 93
Range % 89-97
Recovery
The recovery of human EPHX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 103 99-108
EDTA plasma (n=4) 93 89-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.209 2.326 2.268 2.113
750 1.720 1.664 1.692 1.537
375 1.194 1.213 1.204 1.049
187.5 0.871 0.857 0.864 0.709
93.75 0.546 0.532 0.539 0.384
46.88 0.383 0.401 0.392 0.237
23.44 0.257 0.249 0.253 0.098
0 0.152 0.158 0.155  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human EPHX2 ELISA Kit was designed for the quantitative measurement of Human EPHX2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides. Also determines steady-state levels of physiological mediators.; Bifunctional enzyme. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid. Has phosphatase activity toward lyso-glycerophospholipids with also some lower activity toward lysolipids of sphingolipid and isoprenoid phosphates.
Gene References into Functions
  1. the A allele of exonic polymorphism in EPHX2 rs751141 is negatively associated with the incidence of diabetic nephropathy in the Chinese type 2 diabetes population, which could be modulated by homocysteine level status. PMID: 29629376
  2. Single nucleotide polymorphism of EPHX2 rs751141 is associated with Early Neurological Deterioration. PMID: 28835580
  3. The activity of Ephx2 promoter-coding luciferase was significantly attenuated in HEK293T cells treated with 17beta-E2. PMID: 29295935
  4. hypomethylation of the promoter region of EPHX2 and K55R polymorphism were associated with significant increased risk of pre-eclampsia. PMID: 29058492
  5. Study demonstrated an isolated gene change, sEH R287Q, in a core family of familial hypercholesterolemia (FH). The results showed that sEH R287Q decreased the expression level and hydrolase activity of sEH, decreased the binding of LDL to LDLR and LDL internalization in cultured hepatocyte cell line BEL-7402. PMID: 29665449
  6. Soluble epoxide hydrolase plays role in the choroidal neovascularization PMID: 29193961
  7. Interestingly, rs2279590 locus has a widespread enhancer effect on two nearby genes, protein tyrosine kinase 2 beta (PTK2B) and epoxide hydrolase-2 (EPHX2); both of which have been previously associated with AD as risk factors. PMID: 28973302
  8. Results showed that among type 2 diabetic patients, the combined effect of MTHFR TT and EPHX2 GG or GA + AA genotype has a higher risk of ischemic stroke compared with the control group. PMID: 28409162
  9. we describe the bacterial expression of the recombinant N-terminal domain of sEH-P and the development of a high-throughput screening protocol using a sensitive and commercially available substrate fluorescein diphosphate. The usability of the assay system was demonstrated and novel inhibitors of sEH-P were identified. PMID: 27009944
  10. The WT sEH formed a very tight dimer, with a KD/M in the low picomolar range. Only R287Q resulted in a large change of the KD/M However, human tissue concentrations of sEH suggest that it is always in its dimer form independently of the SNP. PMID: 24771868
  11. The EPHX2 Lys55Arg polymorphism is associated with AKI following cardiac surgery in patients without preexisting CKD PMID: 28552948
  12. EPHX2 polymorphism , might be important determinant of hydrochlorothiazide treatment in patients with hypertension. PMID: 27381900
  13. This study meta-analysis results did not show significant associations between the polymorphisms of EPHX2. PMID: 27266621
  14. EPHX2 Variants are associated with Ischemic Stroke. PMID: 27087514
  15. Because Epoxide Hydrolase 2 (EPHX2) was identified as a novel AN susceptibility gene, and because its protein product, soluble epoxide hydrolase (sEH), converts bioactive epoxides of polyunsaturated fatty acid (PUFA) to the corresponding diols, lipidomic and metabolomic targets of EPHX2 were assessed to evaluate the biological functions of EPHX2 and their role in AN. PMID: 25824304
  16. Increased levels of soluble epoxide hydrolase in the brain of depressed patients PMID: 26976569
  17. carriers of rs1042032 GG genotype had lower estimated glomerular filtration rate and higher serum creatinine values 1 year after grafting compared to patients carrying wildtype A-allele; this GG genotype also associated to increased risk of acute rejection; results suggest genetic variability in EPHX2 may have an impact on outcome of deceased-donor renal transplantation PMID: 26230946
  18. Suggest role for Ephx2 in vascular smooth muscle phenotypic modulation and migration in the development of atherosclerosis. PMID: 26453326
  19. Essential hypertension is associated with the rs751141 G/A allele gene polymorphism in EPHX2 in Han subjects but not in Kazakh and Uygur subjects. PMID: 25966114
  20. Within the EPHX2 promoter -56/+32, five Sp1-binding sites were identified, and the mutation of each of these sites showed that the first one (SP1_1) was important in both suppression by Sp1 and activation by AP2alpha. PMID: 26341485
  21. A genetic variant that results in decreased soluble epoxide hydrolase activity is associated with increased insulin sensitivity. PMID: 25173047
  22. Potent natural soluble epoxide hydrolase inhibitors from Pentadiplandra brazzeana baillon: synthesis, quantification, and measurement of biological activities in vitro and in vivo. PMID: 25659109
  23. Genetic polymorphisms of soluble EH are associated with neurological and vital outcomes after aneurysmal subarachnoid hemorrhage. PMID: 25216066
  24. Findings suggest a novel association of gene variants within EPHX2 to susceptibility to anorexia nervosa and provide a foundation for future study of this important yet poorly understood condition PMID: 23999524
  25. Presence of single nucleotide polymorphism rs751141 of EPHX2 is associated with significantly increased risk of atrial fibrillation recurrence following catheter ablation. PMID: 23711456
  26. Evidence suggests that modulation of plasma levels of epoxyeicosatrienoic acid (EET) (for example, down-regulation of EET in patients with renovascular disease-associated hypertension) is due, in part, to up-regulation of EPHX2. [REVIEW] PMID: 23011468
  27. sEH is a physiological modulator of ER stress and a potential target for mitigating complications associated with obesity PMID: 23576437
  28. the importance of sEH in MCP-1-regulated monocyte chemotaxis PMID: 23160182
  29. Soluble epoxide hydrolase dimerization is required for hydrolase activity PMID: 23362272
  30. The essential hypertension in Kazaks in Xinjiang is not associated with rs751141G/A gene polymorphism, but is associated with rs751141G/A allele gene polymorphism of EPHX2. PMID: 22336502
  31. Upregulation of sEH in proximal tubular cells in chronic proteinuric kidney diseases may mediate proteinuria-induced renal damage. PMID: 23152298
  32. investigation of role of cytoplasmic Ephx2 in diabetic nephropathy by gene disruption techniques: Ephx2 gene inhibition (or added epoxyeicosatrienoic acids) protects renal proximal tubular cell line from tumor necrosis factor-alpha-induced apoptosis. PMID: 22739108
  33. sEH is one of the etiological factors of cardiovascular diseases, and plays an important role in the progression of myocardium ischemia. PMID: 21704394
  34. ATF6 activation and DNA demethylation may coordinately contribute to Hcy-induced sEH expression and endothelial activation. PMID: 22354938
  35. These findings also underline possible cellular mechanisms by which both activities of sEH (EH and phosphatase) may have complementary or opposite roles. PMID: 22387545
  36. This case-control study as well as meta-analysis suggested no association between CYP2J2 G-50T and EPHX2 R287Q and the risk of developing coronary artery disease . PMID: 21642892
  37. In patients with IgA nephropathy, some single nucleotide polymorphisms of EPHX2 were associated with more rapid disease progression. PMID: 21429967
  38. The PLA2G7, HPGD, EPHX2, and CYP4F8 genes are highly expressed in prostate cancer. PMID: 21281786
  39. SP-1 is involved in the decrease in the transcription of sEH as a result of DNA methylation in HepG2 cells, which might contribute to epigenetic mechanism-induced carcinogenesis in hepatocytes. PMID: 20888937
  40. Genetic variation in epoxide hydrolase 2 is associated with forearm vasodilator responses in a bradykinin receptor- and endothelium-independent manner, suggesting an important role for soluble epoxide hydrolase in regulating vascular function in humans. PMID: 21098312
  41. The functional K55R polymorphism of the EPHX2 gene confers a higher risk of hypertension prevalence and increases the risk of incident ischemic stroke in male homozygotes. PMID: 20065888
  42. Results show that no relationship exists between EPHX2 and risk of ischemic stroke, myocardial infarction, and ischemic heart disease. PMID: 19940276
  43. Common polymorphisms within EPHX2 do not appear to be important risk factors for Parkinson's disease. PMID: 11692079
  44. Close association of soluble epoxide hydrolase gene G860A (Arg287Gln) polymorphism with insulin resistance in type 2 diabetic patients. PMID: 15845398
  45. Multiple variants exist within or near the EPHX2 gene, with greatly contrasting relationships to ischemic stroke incidence; some associated with a higher incidence and others with a lower incidence. PMID: 16115816
  46. Soluble epoxide epoxygenase-related SNP is not associated with increased risk of hypertension in the African American population. PMID: 16202848
  47. Consistent with the finding of isoprenoid phosphates as substrates for sEH, we identified isoprenoid-derived N-terminal inhibitors with IC50 values ranging from 0.84 (+/-0.9) to 55.1 (+/-30.7) microM. PMID: 16414022
  48. EPHX2 has an emerging role as a risk factor for atherosclerosis, whose effects are influenced by smoking PMID: 16545818
  49. EPHX2 as a potential cardiovascular disease-susceptibility gene. PMID: 16595607
  50. human EPHX2 mutations may in part explain the genetic variability in sensitivity to ischemic brain injury and stroke outcome PMID: 17460077

Show More

Hide All

Subcellular Location
Cytoplasm. Peroxisome.
Protein Families
AB hydrolase superfamily, Epoxide hydrolase family
Database Links

HGNC: 3402

OMIM: 132811

KEGG: hsa:2053

STRING: 9606.ENSP00000430269

UniGene: Hs.212088

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X