Human Extracellular sulfatase Sulf-1(SULF1) ELISA kit

Instructions
Code CSB-EL022930HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name sulfatase 1
Alternative Names DSulf ELISA Kit; Extracellular sulfatase Sulf 1 ELISA Kit; Extracellular sulfatase Sulf-1 ELISA Kit; Extracellular sulfatase Sulf1 ELISA Kit; HSULF 1 ELISA Kit; HSulf-1 ELISA Kit; Neu5 ELISA Kit; Sulf1 ELISA Kit; SULF1_HUMAN ELISA Kit; Sulfatase FP ELISA Kit; Sulfated ELISA Kit
Abbreviation SULF1
Uniprot No. Q8IWU6
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SULF1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 87-99
1:2 Average % 103
Range % 91-108
1:4 Average % 97
Range % 92-100
1:8 Average % 100
Range % 90-104
Recovery
The recovery of human SULF1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 99 88-104
EDTA plasma (n=4) 97 89-101
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
500 2.496 2.411 2.454 2.301
250 1.915 1.968 1.942 1.789
125 1.284 1.237 1.261 1.108
62.5 0.866 0.850 0.858 0.705
31.2 0.494 0.512 0.503 0.350
15.6 0.349 0.365 0.357 0.204
7.8 0.254 0.261 0.258 0.105
0 0.151 0.155 0.153  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. Diminishes HSPG (heparan sulfate proteoglycans) sulfation, inhibits signaling by heparin-dependent growth factors, diminishes proliferation, and facilitates apoptosis in response to exogenous stimulation.
Gene References into Functions
  1. High SULF1 expression is associated with glioma. PMID: 29360432
  2. These findings suggested that SULF1 could be an indicator of the clinicopathological features and prognosis of pancreatic cancer. PMID: 29843217
  3. Defective autophagy is associated with loss of HSulf-1 in ovarian cancer. PMID: 28169314
  4. our study found that increased SULF1 expression is significantly predictive of more advanced tumor stage and poorer metastasis-free survival and disease-specific survival in patients with both UTUC and UBUC. PMID: 28525382
  5. Data show that sulfatase 1 (hSulf-1) overexpression in melanoma cells can inhibit cell proliferation and induce cell cycle arrest and apoptosis by decreasing the protein kinase B (AKT) phosphorylation and limiting cyclin dependent kinase 4 (CDK4) nuclear import. PMID: 27806323
  6. Sulf-1 is responsive to TNF-alpha stimulation and may function as an autocrine regulator of fibroblast expansion in the course of an inflammatory response PMID: 27693418
  7. The short variants of Sulf1 promoted FGF2-induced MDA-MB231 and MCF7 in vitro growth while full-length Sulf1 inhibited growth supporting in vivo mammary tumour cell signalling patterns of growth. PMID: 27294358
  8. The SULF1/SULF2 activation thus does not only promote regulated foetal growth and injury-induced liver regeneration but also dysregulated tumour growth. PMID: 27013228
  9. Results show that SULF1 or SULF2 overexpression contributes to colorectal cancer cell proliferation, migration, and invasion. PMID: 25477293
  10. Data suggest that Sulfatase 1 (hSulf-1) may be a suitable target for cancer therapy. PMID: 24970807
  11. findings show an upregulation of SULF1 in degenerative discs for the first time, and suggest that there is a link between SULF1 and disc degeneration PMID: 25469740
  12. rs6990375 polymorphism of SULF1 gene could be one of the factors related to recurrent miscarriage in Iranian women. PMID: 24322345
  13. identification of markers including SULF1 may improve detection of this disease at its earliest stages improving patient treatment and prognosis PMID: 24911625
  14. SULF1/SULF2 splice variants regulate pancreatic tumor progression. PMID: 24726914
  15. Knockdown of SULF2 in human corneal epithelial cell line slowed migration, which was restored by overexpression of either mouse SULF2 or human SULF1. PMID: 23950901
  16. Strong interaction depends on the presence of Sulf1-substrate groups. PMID: 23891937
  17. Ectopic expression of SULF1 or SULF2 in HeLa cells, which decreases cell surface heparan sulfate proteoglycan sulfation, diminished Chlamydia muridarum binding and decreased vacuole formation. PMID: 23480519
  18. these observations provide evidence that HPEI nanogels delivering HSulf-1 combined with DDP may have a promising application in the therapy of human ovarian cancer. PMID: 22825572
  19. miR-21-mediated suppression of both hSulf-1 and PTEN led to activation of AKT/ERK pathways and epithelial-mesenchymal transition in hepatocellular carcinoma, promoting tumor growth. PMID: 23684551
  20. HSulf-1 and HSulf-2 share the same desulfation processs but with a different rate PMID: 23457216
  21. inhibits proliferation and invasion of esophageal squamous cell carcinoma by decreasing heparin-binding growth factor signaling PMID: 23053899
  22. HSULF-1 is expressed at lower levels in H292 lung cancer cells than in normal human alveolar cells and that its over-expression reduced cell viability in H292 cells by inducing apoptotic pathways, at least in part by inhibiting ERK/Akt signaling PMID: 22873647
  23. SULF1 may serve as a promising biomarker for patients with gastric carcinoma. PMID: 22653794
  24. Sulf-1 protein expression is down-regulated in gastric cancer. PMID: 22524839
  25. extracellular HSulf-1 may function as a negative regulator of proliferation and invasion in gastric cancer by suppressing Wnt/beta-catenin signaling at the cell surface. PMID: 21722266
  26. SULF1 and SULF2 are overexpressed in various human cancer types and can be associated to progression and prognosis. PMID: 21599997
  27. Data show that HSulf-1 depletion in breast cancer cells resulted in an increased and sustained bFGF2 (basic fibroblast growth factor) signaling and promoted cell migration and invasion under hypoxic conditions. PMID: 21266348
  28. Phage microarrays containing colorectal cancer cDNA libraries were prepared to identify phage-expressed peptides recognized by tumor-specific autoantibodies from patient sera. PMID: 21228115
  29. Genetic variations in SULF1 may play a role in ovarian cancer onset and prognosis. PMID: 21214932
  30. Haploinsufficiency of SULF1 combined with haploinsufficiency of SLCO5A1 (or the altered expression of a neighboring gene through position effect) could be necessary in the pathogenesis of MSS. PMID: 20602915
  31. The expression levels of four of the up-regulated genes, CXCL1, SPARC, SPP1 and SULF, were significantly higher in the cancerous tissue compared with the normal tissue (fold change 3.4-8.9). PMID: 19780053
  32. Sulfs are extracellular endosulfatases with strong potential for modulating the interactions of heparan sulfate proteoglycans in the extracellular microenvironment PMID: 12368295
  33. modulates signaling by heparin-binding growth factors, and HSulf-1 down-regulation represents a novel mechanism by which cancer cells can enhance growth factor signaling PMID: 12686563
  34. Down-regulation of hSulf1 contributes to hepatocarcinogenesis by enhancing heparin-binding growth factor signaling and resistance to apoptosis. PMID: 14699503
  35. High expression of Hsulf-1 occurs in the stromal elements as well as in the tumor cells in pancreatic cancer and interferes with heparin-binding growth factor signaling PMID: 15817123
  36. HSulf-1 and HSulf-2 have roles in inhibiting myeloma tumor growth PMID: 16192265
  37. Loss of HSulf-1 expression is associated with breast cancer PMID: 17363371
  38. Sulf1 is a TGF-beta1-responsive gene both in vitro and in vivo and may function as a negative regulator of TGF-beta1-induced fibrogenesis PMID: 18503048
  39. Increased sulf-1 expression may change the sulfation patterns of heparan sulfate proteoglycans and growth factor activities and thus contribute to abnormal chondrocyte activation and cartilage degradation in osteoarthritis. PMID: 18507859
  40. Sulf1 and Sulf2 are two heparan sulfate 6-O-endosulfatases that regulate the activity of multiple growth factors, such as fibroblast growth factor and Wnt, and are essential for mammalian development and survival PMID: 18687675
  41. detection of the HSulf-1 promoter methylation in serum samples may have clinical implications in early detection and diagnosis of human breast and gastric cancers. PMID: 19006069
  42. Strategies targeting sulfatase 1(SULF1) or the interaction between SULF1 and the related sulfatase 2 will potentially be important in developing novel cancer therapies. PMID: 19373441
  43. vHNF1 acts as a repressor of HSulf-1 expression and might be a molecular target for ovarian cancer therapy. PMID: 19487294
  44. analysis of the subdomain organization of sulf-1 and sulf-2 PMID: 19520866
  45. Data show that specific regions of the Sulf1 hydrophilic domain (HD) influence different aspects of heparan sulfate binding, cellular localization, and enzyme function. PMID: 19666466
  46. HSulf-1 (SULF1) and HSulf-2 (SULF2) are potent inhibitors of myeloma tumor growth in vivo. PMID: 16192265

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Subcellular Location Endoplasmic reticulum, Golgi apparatus, Golgi stack, Cell surface
Protein Families Sulfatase family
Tissue Specificity Expressed at highest levels in testis, stomach, skeletal muscle, lung, kidney, pancreas, small intestine and colon. It is also detected in normal ovarian surface epithelial cells. Down-regulation seen in ovarian carcinoma cell lines, ovarian cancers, brea
Database Links

HGNC: 20391

OMIM: 610012

KEGG: hsa:23213

STRING: 9606.ENSP00000260128

UniGene: Hs.409602

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