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Human Fetuin-B(FETUB) ELISA kit

Human Fetuin-B(FETUB) ELISA kit

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Citations (2) Protocol MSDS

Code	CSB-EL008598HU
Size	96T,5×96T,10×96T
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Product Details
Citations (2)
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Target Background

Product Details

Alternative Names

16G2 ELISA Kit; 2310011O17Rik ELISA Kit; AI255764 ELISA Kit; D17980 ELISA Kit; Fet ELISA Kit; Fetub ELISA Kit; FETUB_HUMAN ELISA Kit; Fetuin beta ELISA Kit; Fetuin-B ELISA Kit; Fetuin-like protein ELISA Kit; Fetuin-like protein IRL685 ELISA Kit; Gugu ELISA Kit; IRL685 ELISA Kit; Pp63 ELISA Kit

Abbreviation

FETUB

Uniprot No.

Q9UGM5

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.137 ng/ml-100 ng/ml.

Sensitivity

0.137 ng/ml.

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	12.720	13.079	12.874	12.643	12.823	12.925
SD	0.023	0.027	0.025	0.036	0.033	0.031
CV(%)	3.622	4.160	3.900	5.696	5.164	4.821

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Fetuin B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:10	Average %	106
	Range %	102-108
1:20	Average %	88
	Range %	84-92
1:40	Average %	92
	Range %	85-96
1:80	Average %	87
	Range %	82-83

Recovery

The recovery of human Fetuin B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	104	98-108
EDTA plasma (n=4)	98	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
100	2.775	2.859	2.817	2.665
33.33	1.951	1.937	1.944	1.792
11.11	1.090	1.062	1.076	0.924
3.7	0.642	0.691	0.666	0.514
1.23	0.385	0.364	0.375	0.223
0.41	0.283	0.285	0.284	0.132
0.137	0.218	0.235	0.226	0.074
0	0.151	0.153	0.152

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The product CSB-EL008598HU is a sandwich ELISA kit developed to measure levels of human Fetuin-B (FETUB) in multiple samples, including serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of FETUB in samples. The color intensity is measured at 450 nm via a microplate reader.

FETUB is a highly selective and potent inhibitor of metallopeptidases (MPs) of the astacin family. It is a liver-derived plasma protein with serum concentrations of about 0.01 g/l in humans. FETUB plays an essential role in female fertility. By blocking ovastacin, fetuin-B counteracts premature zona pellucida (ZP) hardening before fertilization and therefore maintains female fertility by keeping the ZP penetrable to sperm. Studies have found that FETUB is elevated in type 2 diabetes patients and FETUB knockdown improves glucose metabolism in obese mice. FETUB is also an inhibitor of basic calcium phosphate, blocking unwanted calcification.

Citations

Fetuin B alleviates testosterone propionate-induced oxidative stress and mitochondrial dysfunction in KGN cells by upregulating the TGFR2/SMAD3 pathway Y Gao,ResearchSquare,2022
Proteomic Analysis of Serum from Patients with Major Depressive Disorder to Compare Their Depressive and Remission Statuses Jiyeong Lee et al, Psychiatry Investig,2015

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Target Background

Function
(From Uniprot)

Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening.

Gene References into Functions

High Serum fetuin-B expression is associated with insulin resistance. PMID: 29194974
fetuin B may serve as a potential biomarker for coronary artery disease PMID: 28822077
Increased serum fetuin-B level was significantly associated with higher risk of insulin resistance. Common genetic variants of FETUB SNPs (rs4686434, rs6785067, and rs3733159) were significantly associated with serum fetuin-B concentrations but were not associated with insulin resistance. PMID: 29390354
serum level of fetuin B in the control subjects was lower than it in nonalcoholic fatty liver disease patients. fetuin B correlated with diastolic blood pressure, serum alanine transaminase and triglycerides, among the controls. It suggested a potential association between serum fetuin B and the presence of NAFLD. PMID: 27628583
Further studies need to elucidate factors contributing to fetuin B regulation in humans, as well as the pathophysiological significance of fetuin B upregulation in gestational diabetes PMID: 28183456
Data indicate that fetuin-B was one of the highly expressed serum proteins in acute myocardial infarction. PMID: 25671698
Reduced expression of fetuin-b led to a significant increase in the expression of lipogenic genes, thereby resulting in higher lipid levels. PMID: 22739111
serum fetuin has a role in processing and transport of matrix gamma-carboxyglutamic acid protein and bone morphogenetic protein-2 in cultured human vascular smooth muscle cells PMID: 15280384
results support the hypothesis that fetuin-A serum levels are associated with bone cell activity PMID: 16797392

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Subcellular Location

Secreted.

Protein Families

Fetuin family

Tissue Specificity

Liver and testis.

Database Links

HGNC: 3658

OMIM: 605954

KEGG: hsa:26998

STRING: 9606.ENSP00000265029

UniGene: Hs.81073

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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